Photosynthetica 1997, 33(11):57-65 | DOI: 10.1023/A:1006811817403

Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis

Guang Ce Wang1, B.C. Zhou1, C.K. Tseng1
1 Experimental Marine Biology Laboratory, Institute of Oceanology, Chinese Academy of Sciences, Qingdao

C-phycocyanin (CPC) and allophycocyanin (APC) were purified from Spirulina platensis, then the CPC was attached covalently to the APC by reacting their ∈-amino groups. The excitation energy could be transferred from the CPC to the APC in the CPC-APC conjugate. Intact phycobilisomes (PBS), consisting of CPC, APC, colourless linker polypeptides, and APC B or Lcm, were isolated from S. platensis. Spectroscopic properties of the isolated PBSs kept at 20 °C for various times showed that the connection between the APC and the APC B or Lcm was looser than that between the CPC and the APC in the isolated PBSs. The CPC-APC conjugate was more stable than the isolated PBSs, and the linker polypeptides had a minor influence on the excitation energy transfer characteristic between different phycobiliproteins in the PBS.

Additional key words: absorption and fluorescence emission and excitation spectra; covalent bond; linker polypeptides

Prepublished online: January 1, 1998; Published: January 1, 1997  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Wang, G.C., Zhou, B.C., & Tseng, C.K. (1997). Spectroscopic properties of the C-phycocyanin-allophycocyanin conjugate and the isolated phycobilisomes from Spirulina platensis. Photosynthetica, 34(1), 57-65. doi: 10.1023/A:1006811817403
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Bhalerao, R.P., Gillbro, T., Gustafsson, P.: Structure and energy transfer of the phycobilisome in a linker protein replacement mutant of cyanobacterium Synechococcus 7942.-Biochim. biophys. Acta 1060: 59-66, 1991. Go to original source...
    2. Glazer, A.N.: Photosynthetic accessory proteins with bilin prosthetic groups.-In: Hatch, M.D., Boardman, N.K. (ed.): The Biochemistry of Plants. A Comprehensive Treatise. Vol. 8. Pp. 51-96. Academic Press, New York-London-Toronto-Sydney-San Francisco 1981. Go to original source...
    3. Glazer, A.N.: Phycobilisome. A macromolecular complex optimized for light energy transfer.-Biochim. biophys. Acta 768: 29-51, 1984. Go to original source...
    4. Glazer, A.N.: Light harvesting by phycobilisomes.-Annu. Rev. Biophys. biophys. Chem. 14: 47-77, 1985. Go to original source...
    5. Gantt, E., Lipschultz, C.A.: Phycobilisomes of Porphyridium cruentum: Pigment analysis.-Biochemistry 13: 2960-2966, 1974. Go to original source...
    6. Holzwarth, A.R.: Structure-function relationships and energy transfer in phycobiliprotein antennae.-Physiol. Plant. 83: 518-528, 1991. Go to original source...
    7. Hurn, B.A.L., Chantler, S.M.: Production of reagent antibodies.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 70. Pp. 104-142. Academic Press, New York-London-Toronto-Sydney-San Francisco 1980.
    8. Maxson, P., Sauer, K., Zhou, J., Bryant, D.A., Glazer, A.N.: Spectroscopic studies of cyanobacterial phycobilisomes lacking core polypeptides.-Biochim. biophys. Acta 977: 40-51, 1989. Go to original source...
    9. Menon, V.K.N., Kumar, A., Menon, A.L., Varma, A.K.: A rapid procedure for the isolation of phycobilisomes from cyanobacteria.-Anal. Biochem. 172: 377-381, 1988. Go to original source...
    10. Reichlin, M.: Use of glutaraldehyde as a coupling agent for proteins and peptides.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 70. Pp. 159-165. Academic Press, New York-London-Toronto-Sydney-San Francisco 1980. Go to original source...
    11. Wang, G., Zhou, B.C., Tseng, C.K.: The excitation energy transfer in an artificial R-phycoerythrin-allophycocyanin conjugate.-Photosynthetica 32: 609-612, 1996.

    Return to the content