Photosynthetica 1997, 33(13):357-366 | DOI: 10.1023/A:1006855816437

Physiological significance of proline and glycinebetaine: Maintaining photosynthesis during NaCl stress in wheat

L.R. Rajasekaran1, P.E. Kriedemann2, D. Aspinall2, L.G. Paleg2
1 Faculty of Forestry, University of Toronto, Toronto, Ontario, Canada
2 CSIRO Division of Forestry Research, Canberra, Australia

Experiments on the physiological significance of accumulation of proline and glycinebetaine (GB) in sustaining photosynthesis during salt stress in wheat in vivo showed that pre-treatment with GB, but not proline, alleviated NaCl-induced stomatal and non-stomatal inhibition of photosynthesis completely. A permeating and non-dissociating osmoticum, 3-orthomethyl-glucopyranose, also alleviated NaCl-induced perturbations of photosynthesis, suggesting that GB may work by maintaining chloroplast volume and not by specific effects on photosynthetic processes.

Additional key words: assimilation rate; CO2; intercellular CO2 partial pressure; 3-orthomethyl-glucopyranose; stomatal conductance

Prepublished online: January 1, 1998; Published: September 1, 1997  Show citation

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Rajasekaran, L.R., Kriedemann, P.E., Aspinall, D., & Paleg, L.G. (1997). Physiological significance of proline and glycinebetaine: Maintaining photosynthesis during NaCl stress in wheat. Photosynthetica, 34(3), 357-366. doi: 10.1023/A:1006855816437
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    References

    1. Badger, M.R., Sharkey, T.D., Caemmerer, S. von: The relationship between steady-state gas exchange of bean leaves and the levels of carbon-reducing-cycle intermediates.-Planta 160: 305-313, 1984. Go to original source...
    2. Ball, M.C., Farquhar, G.D.: Photosynthetic and stomatal responses of two mangrove species, Aegiceras corniculatum and Avicennia maritima, to long term salinity and humidity conditions.-Plant Physiol. 74: 1-6, 1984. Go to original source...
    3. Berry, J.A., Downton, W.J.S.: Environmental regulation of photosynthesis.-In: Govinjee (ed.): Photosynthesis: Development, Carbon Metabolism and Plant Productivity. Vol. II. Pp. 263-343. Academic Press, New York-London-Paris-San Diego-San Francisco-São Paulo-Sydney-Tokyo-Toronto 1982.
    4. Caemmerer, S. von, Farquhar, G.D.: Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves.-Planta 153: 376-378, 1981. Go to original source...
    5. Coughlan, J.S., Wyn Jones, R.G.: Some responses of Spinacia oleraceae to salt stress.-J. exp. Bot. 31: 883-893, 1980. Go to original source...
    6. Critchley, C.: Stimulation of photosynthetic electron transport in a salt tolerant plant by high chloride concentrations.-Nature 298: 483-485, 1982. Go to original source...
    7. Davis, W.J., Metcalfe, J., Lodge, T.A., Da Costa, A.R.: Plant growth substances and the regulation of growth under drought.-Aust. J. Plant Physiol. 13: 105-126, 1986. Go to original source...
    8. Downton, W.J.S.: Photosynthesis in salt stressed grapevines.-Aust. J. Plant Physiol. 4: 183-192, 1977. Go to original source...
    9. Downton, W.J.S., Millhousc, J.: Turgor maintenance during salt stress prevents loss of variable fluorescence in grapevine leaves.-Plant Sci. Lett. 31: 1-7, 1983. Go to original source...
    10. Farquhar, G.D., Caemmerer, S. von: Modcling of photosynthetic response to environmental conditions.-In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Physiological Plant Ecology II. Water Relations and Carbon Assimilation. Pp. 549-587. Springer-Verlag, Berlin-Heidelberg-New York 1982. Go to original source...
    11. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and photosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    12. Fedina, I.S., Popova, A.V.: Photosynthesis, photorespiration and proline accumulation in water-stressed pea leaves.-Photosynthetica 32: 213-220, 1996.
    13. Flowers, S.T.J., Troke, P.F., Yeo, A.R.: The mechanism of salt tolerance in halophytes.-Annu. Rev. Plant Physiol. 28: 89-121, 1977. Go to original source...
    14. Gale, J., Kohl, H.C., Hagan, R.M.: Changes in the water balance and photosynthesis of onion, bean and cotton plants under saline conditions.-Physiol. Plant. 20: 408-420, 1967. Go to original source...
    15. Greenway, H., Munns, R., Gibbs, J.: Effects of accumulation of 3-O-methyl glucose levels of endogenous osmotic solutes in Chlorella emersonii.-Plant Cell Environ. 5: 405-412, 1982. Go to original source...
    16. Grumet, R., Hanson, A.D.: Genetic evidence for an osmo regulatory function of glycinebetaine accumulation in barley.-Aust. J. Plaut Physiol. 13: 353-364, 1986. Go to original source...
    17. Hanson, A.D., May, A.M., Grumet, R., Bode, J., Jamieson, G.C., Rhodes, D.: Betaine synthesis in chenopods: localization in chloroplasts.-Proc. nat. Acad. Sci. USA 82: 3678-3682, 1985. Go to original source...
    18. Incharoensakdi, A., Takabe, T., Akazawa, T.: Effect of betain on enzyme activity and subunit interaction of ribulose-1,5-biphosphate carboxylase/oxygenase from Aphanothece halophytica.-Plant Physiol. 81: 1044-1049, 1986. Go to original source...
    19. Itai, C., Vaadia, Y.: Kinetin like activity in root exudate of water stressed sunflower plants.-Physiol. Plant. 18: 941-944, 1965. Go to original source...
    20. Jagels, R.: Further evidence for osmoregulation in epidermal leaf cells of seagrasses.-Amer. J. Bot. 70: 327-333, 1983. Go to original source...
    21. Jolivet, Y., Haelin, J., Larher, F.: Osmoregulation in halophytic higher plant: The protective effects of glycinebetaine and other related sources against oxalate destabilization of membranes in beet root cells.-Z. Pflanzenphysiol. 109: 171-230, 1983. Go to original source...
    22. Jones, R.J., Mansfield, T.A.: Effects of abscisic acid and its esters on stomatal aperture and the transpiration ratio.-Physiol. Plant. 26: 321-327, 1972. Go to original source...
    23. Nash, D., Paleg, L.G., Wiskich, J.T.: The effect of proline, betain and some other solutes on the heat stability of mitochondrial enzyme.-Aust. J. Plant. Physiol. 9: 47-57, 1982. Go to original source...
    24. Noguchi, M., Kowai, A., Tanaki, E.: Studies on nitrogen metabolism in tobacco plants. VIII. Δ' pyrroline-5-carboxylate reductase from tobacco leaves.-Agr. biol. Chem. 30: 492-496, 1966. Go to original source...
    25. Oaks, A., Michael, D.J., Barnard, R.A., Johnson, E.J.: The regulation of proline biosynthesis in maize roots.-Can. J. Bot. 48: 2249-2258, 1970. Go to original source...
    26. Paleg, L.G., Douglas, T.J., Van Dall, A., Keech, D.B.: Proline and betaine protect enzymes against heat inactivation.-Aust. J. Plant Physiol. 8: 107-114, 1981. Go to original source...
    27. Paleg, L.G., Stewart, G.R., Bradbeer, J.W.: Proline and glycinebetaine influenced protein salvation.-Plant Physiol. 75: 974-978, 1984. Go to original source...
    28. Pollard, A., Wyn Jones, R.G.: Enzyme activities in concentrated solutions of glycinebetaine and other solutes.-Planta 144: 291-298, 1979. Go to original source...
    29. Rajasekaran, L.R.: The Mechanism of Accumulation of Proline and Quaternary Ammonium Compounds and Their Adaptive Significance in Tomato (Lycopersicon esculentum Mill) and Wheat (Triticum aestivum) Exposed to Salt Stress.-Ph.D. Thesis. University of Adelaide, Glen Osmond 1988.
    30. Raschke, K.: Movements of stomata.-In: Haupt, W., Feinleib, M.E. (ed.): Physiology of Movements. Pp. 383-441. Springer-Verlag, Berlin-Heidelberg-New York 1979.
    31. Robinson, S.P., Joncs, G.P.: Accumulation of glycinebetaine in chloroplasts provides osmotic adjustment during salt stress.-Aust. J. Plant Physiol. 13: 659-668, 1986. Go to original source...
    32. Seemann, J.R., Berry, J.A.: Interspecific differences in the kinetic properties of RuBP carboxylase protein.-Carnegie Inst. Washington Year Book 81: 78-83, 1982.
    33. Seemann, J.R., Critchley, C.: Effects of salt stress on the growth, ion content, stomatal behaviour and photosynthetic capacity of a salt sensitive species. Phaseolus vulgaris L.-Planta 164: 151-162, 1985. Go to original source...
    34. Stewart, C.R.: Effect of proline and carbohydrates on metabolism of exogeneous proline by excised bean leaves in the dark.-Plant Physiol. 50: 551-555, 1972. Go to original source...
    35. Stewart, C.R., Lee, F.: Δ'-pyroline-5-carboxylic acid DH in mitochondria preparations from plant seedlings.-Plant Sci. Lett. 3: 173-181, 1974. Go to original source...
    36. Storey, R., Wyn Jones, R.G.: Quarternary ammonium compounds in plants in relation to salt resistance.-Phytochemistry 66: 447-453, 1977. Go to original source...
    37. Terashima, I., Wong, S.-C., Osmond, C.B., Farquhar, G.D.: Characterisation of non-uniform photosynthesis induced by abscisic acid in leaves having different mesophyll anatomies.-Plant Cell Physiol. 29: 385-394, 1988.
    38. Walker, M.A., Dumbroof, E.B.: Effects of salt stress on abscisic acid and cytokinin levels in tomato.-Z. Pflanzenphysiol. 101: 461-470, 1981. Go to original source...
    39. Walker, R.A., Törökfalvy, E., Downton, W.J.S.: Photosynthetic responses of the citrus varieties rangput lime and etong citron to salt treatment.-Aust. J. Plant Physiol. 9: 783-790, 1983. Go to original source...
    40. West, D.W., Hoffman, G.J., Fisher, M.J.: Photosynthesis, leaf conductance, and water relations of cowpea under saline conditions.-Irrig. Sci. 7: 183-193, 1986. Go to original source...
    41. Wyn Jones, R.G., Storey, R.: Betains-In: Paleg, L.G., Aspinall, D. (ed.): The Physiology and Biochemistry of Drought Resistance in Plants. Pp. 171-204. Academic Press, Sydney 1981.
    42. Yeo, A.R., Capron, S.J.M., Flowers, T.J.: The effect of salinity upon photosynthesis in rice (Oryza sativa, L.) gas exchange by individual leaves in relation to their salt content.-J. exp. Bot. 36: 1240-1248, 1985. Go to original source...

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