Photosynthetica 1998, 35(1):79-88 | DOI: 10.1023/A:1006873932486

The influence of phosphate deficiency on photosynthesis, respiration and adenine nucleotide pool in bean leaves

M. Mikulska1, J.-L. Bomsel2, A.M. Rychter1
1 Institute of Experimental Plant Biology, University of Warsaw, Warsaw, Poland
2 Laboratoire des Membranes Biologiques, Université Denis Diderot, Paris Cedex 05, France

The decrease in inorganic phosphate (Pi) content of 10-d-old Phaseolus vulgaris L. plants did not affect rates of photosynthesis (PN) and respiration (RD), leaf growth, and adenylate concentration. Two weeks of phosphate starvation influenced the ATP content and leaf growth more than PN and RD. The ATP concentration in the leaves of 15- and 18-d-old phosphate deficient (-P) plants after a light or dark period was at least half of that in phosphate sufficient (+P, control) plants. Similar differences were found in fresh and dry matter of leaves. However, PN declined to 50 % of control in 18-d-old plants only. Though the RD of -P plants (determined as both CO2 evolution and O2 uptake) did not change, an increased resistance of respiration to KCN and higher inhibition by SHAM (salicylhydroxamic acid) suggested a higher engagement of alternative pathway in respiration and a lower ATP production. The lower demand for ATP connected with inhibition of leaf growth may influence the ATP producing processes and ATP concentration. Thus, the ATP concentration in the leaves depends stronger on Pi content than on PN and RD.

Additional key words: adenylate; ADP; AMP; ATP; CO2 compensation concentration; dry matter; KCN; Phaseolus vulgaris; plant age; salicylhydroxamic acid

Prepublished online: March 1, 1998; Published: January 1, 1998  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Mikulska, M., Bomsel, J.-L., & Rychter, A.M. (1998). The influence of phosphate deficiency on photosynthesis, respiration and adenine nucleotide pool in bean leaves. Photosynthetica, 35(1), 79-88. doi: 10.1023/A:1006873932486
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Atkin, O.W., Villar, R., Lambers, H.: Partitioning of electrons between the cytochrome and alternative pathways in intact roots.-Plant Physiol. 108: 1179-1183, 1995. Go to original source...
    2. Azcon-Blero, J., Day, D.A., Lambers, H.: The regulation of respiration in the dark in wheat leaf slices.-Plant Sci. Lett. 32: 313-320, 1983. Go to original source...
    3. Dietz, K.-J., Heilos, L.: Carbon metabolism in spinach leaves as affected by leaf age and phosphorus and sulphur metabolism.-Plant Physiol. 93: 1219-1225, 1990. Go to original source...
    4. Fiske, C.H., Subbarow, Y.: The colorimetric determination of phosphorus.-J. biol. Chem. 66: 375-400, 1925. Go to original source...
    5. Foyer, C., Spencer, C.: The relationship between phosphate status and photosynthesis in leaves. Effects of intracellular orthophosphate distribution, photosynthesis and assimilate partitioning.-Planta 167: 369-375, 1986. Go to original source...
    6. Fredeen, A.L., Raab, T.K., Rao, I.M., Terry, N.: Effects of phosphorus nutrition on photosynthesis in Glycine max (L.) Merr.-Planta 181: 399-405, 1990. Go to original source...
    7. Furbank, R.T., Foyer, C.H., Walker, D.A.: Regulation of photosynthesis in isolated spinach chloroplasts during orthophosphate limitation.-Biochim. biophys. Acta 894: 552-561, 1987. Go to original source...
    8. Giersch, C., Robinson, S.P.: Regulation of photosynthetic carbon metabolism during phosphate limitation of photosynthesis in isolated spinach chloroplasts.-Photosynth. Res. 14: 211-227, 1987. Go to original source...
    9. Harley, P.C., Sharkey, T.D.: An improved model of C3 photosynthesis at high CO2: Reversed O2 sensitivity explained by lack of glycerate reentry into the chloroplast.-Photosynth. Res. 27: 169-178, 1991. Go to original source...
    10. Hauschild, T., Ciereszko, I., Maleszewski, S.: Influence of phosphorus deficiency on post-irradiation burst of CO2 from bean (Phaseolus vulgaris L.) leaves.-Photosynthetica 32: 1-9, 1996.
    11. Heber, U., Heldt, H.W.: The chloroplast envelope: Structure, function, and role in leaf metabolism.-Annu. Rev. Plant Physiol. 32: 139-168, 1981. Go to original source...
    12. Hoefnagel, N.H.N., Millai, A.H., Wiskich, J.T., Day, D.A., Cytochrome and alternative respiratory pathways compete for electrons in the presence of pyruvate in soybean mitochondria.-Arch. Biochem. Biophys. 318: 394-400, 1995. Go to original source...
    13. Horgan, J.M., Wareing, P.F.: Cytokinins and growth responses of seedlings of Betula pendula Roth. and Acer pseudoplatanus L. to nitrogen and phosphorus deficiency.-J. exp. Bot. 31: 525-532, 1980. Go to original source...
    14. Jacob, J., Lawlor, D.W.: Extreme phosphate deficiency decreases the in vivo CO2/O2 specificity factor of ribulose 1,5-bisphosphate carboxylase-oxygenase in intact leaves of sunflower.-J. exp. Bot. 44: 1635-1641, 1993. Go to original source...
    15. Kondracka, A., Rychter, A.M.: The role of Pi recycling processes during photosynthesis in phosphate-deficient bean plants.-J. exp. Bot. 48: 1461-1468, 1997. Go to original source...
    16. Krömer, S.: Respiration during photosynthesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 46: 45-70, 1995. Go to original source...
    17. Miginiac-Maslow, M., Hoarau, A.: Variations in adenylate levels during phosphate depletion in isolated soybean cells and wheat leaf fragments.-Z. Pflanzenphysiol. 107S: 427-436, 1982. Go to original source...
    18. Miginiac-Maslow, M., Nguyen, J., Hoarau, A.: Adenylate metabolism in phosphate-depleted isolated soybean leaf cells and wheat leaf fragments.-J. Plant Physiol. 123: 69-77, 1986. Go to original source...
    19. Möller, I.M., Berezi, A., van der Plas, L.H.W., Lambers, H.: Measurement of the activity and capacity of the alternative pathway in intact plant tissues: Identification of problems and possible solutions.-Physiol. Plant. 72: 642-649, 1988. Go to original source...
    20. Pradet, A.: Étude des adenosine-5 mono-, di-, et tri phosphate dans les tissues végétaux. I. Dosage enzymatique.-Physiol. vég. 5: 209-221, 1967.
    21. Pradet, A., Raymond, P.: Adenine nucleotide ratios and adenylate energy charge in energy metabolism.-Annu. Rev. Plant Physiol. 34: 199-224, 1983. Go to original source...
    22. Rao, I.M., Arulanantham, A.R., Terry, N.: Leaf phosphate status, photosynthesis and carbon partitioning in sugar beet. II. Diurnal changes in sugar phosphates, adenylates, and nicotinamide nucleotides.-Plant Physiol. 90: 820-826, 1989. Go to original source...
    23. Rao, I.M., Terry, N.: Leaf phosphate status, photosynthesis, and carbon partitioning in sugar beet. I. Changes in growth, gas exchange, and Calvin cycle enzymes.-Plant Physiol. 90: 814-819, 1989. Go to original source...
    24. Rufty, T.W., Jr., Israel, D.W., Volk, R.J., Qiu, J., Sa, T.: Phosphate regulation of nitrate assimilation in soybean.-J. exp. Bot. 44: 879-891, 1993. Go to original source...
    25. Rychter, A.M., Chauveau, M., Bomsel, J.L., Lance, C.: The effect of phosphate deficiency on mitochondrial activity and adenylate levels in bean roots.-Physiol. Plant. 84: 80-86, 1992. Go to original source...
    26. Rychter, A.M., Ciesla, E., Kacperska, A.: Participation of cyanide resistance pathway in respiration of winter rape leaves as affected by plant cold acclimation.-Physiol. Plant. 73: 299-304, 1988. Go to original source...
    27. Rychter, A.M., Mikulska, M.: The relationship between phosphate status and cyanide resistant respiration in bean roots.-Physiol. Plant. 79: 663-667, 1990. Go to original source...
    28. Rychter, A.M., Randall, D.D.: The effect of phosphate deficiency on carbohydrate metabolism in bean roots.-Physiol. Plant. 91: 383-388, 1994. Go to original source...
    29. Sicher, R.C., Kremer, D.E.: Effects of phosphate deficiency on assimilate partitioning in barley seedlings.-Plant Sci. 57: 9-17, 1988. Go to original source...
    30. Thorsteinsson, B., Tillberg, J.-E.: Carbohydrate partitioning, photosynthesis and growth in Lemna gibba G3. II. Effects of phosphorus limitation.-Physiol. Plant. 71: 271-276, 1987. Go to original source...
    31. Usuda, H., Shimogawara, K.: Phosphate deficiency in maize. IV. Changes in amounts of sucrose phosphate synthase and PEP carboxylase during the course of phosphate deprivation.-Plant Cell Physiol. 34: 767-770, 1993. Go to original source...
    32. Wagner, A.M., Krab, K.: The alternative respiration pathway in plants, Role and regulation.-Physiol. Plant. 95: 318-325, 1995. Go to original source...
    33. Walker, D.A., Sivak, M.N.: Can phosphate limit photosynthetic carbon assimilation in vivo?-Physiol. vég. 23: 829-841, 1985. Go to original source...

    Return to the content