Photosynthetica 1998, 35(3):369-379 | DOI: 10.1023/A:1006960201513

Analysis of Chlorophyll Fluorescence Decays of Isolated Thylakoids in Various Stages of Greening

A. Kowalczyk1, A. Waloszek2, D. Frackowiak2
1 Institute of Physics of the Nicholas Copernicus University, Toruń, Poland
2 Institute of Molecular Biology, Jagiellonian University, Kraków, Poland

The decay of chlorophyll (Chl) fluorescence of etiochloroplasts isolated in various stage of greening of cucumber cotyledons was analysed in order to get structural information on a photosynthetic apparatus. Two model decays, multiexponential and stretched exponential, were applied in the analysis. The quality of fit in these two models was different in various stages of chloroplast greening. The two-exponent model did not provide a good fit at early greening stages. To improve the fit it was necessary to introduce an additional third component which became very low at later stages. However, chloroplasts in the early stage of greening could also be described by a stretched exponential with parameters indicating rather planar (two-dimensional) arrangement of donor and acceptor molecules. The chloroplasts treated by DCMU and/or photooxidized by strong irradiance exhibit a similar character of fractal decay as untreated samples but in the multiexponential model the exact values of lifetimes and amplitudes of components vary. This suggests that the structure of investigated system does not dramatically change as a result of these two types of treatment.

Additional key words: chloroplast; Cucumis sativus; 3-(3,4-dichlorophenyl)-1,1-dimethyl urea; etiochloroplast; fractal model; photooxidation

Prepublished online: September 1, 1998; Published: January 1, 1998  Show citation

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Kowalczyk, A., Waloszek, A., & Frackowiak, D. (1998). Analysis of Chlorophyll Fluorescence Decays of Isolated Thylakoids in Various Stages of Greening. Photosynthetica, 35(3), 369-379. doi: 10.1023/A:1006960201513
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