Photosynthetica 1998, 35(4):545-550 | DOI: 10.1023/A:1006978907364

Influence of the Degree of Solvent Impurity on the Spectrophotometric Determination of Chlorophylls in 80 % Aqueous Acetone and Dimethyl formamide. Application to Non-Abrasive Extraction of Leaves of Citrus Aurantium

A.L. García1, N. Nicolás1
1 Department of Agricultural Chemistry, University of Murcia, Spain

Chlorophyll (Chl) a and Chl b absorbances were determined in extracts from leaves of Citrus aurantium L. using the solvents diethyl ether (100 %), buffered 80 % aqueous acetone, and N,N'-dimethyl formamide (DMF) (99.83 %). The respective standard deviations (σ) and coefficients of variability (CV) were used to establish the errors obtained when using impure solvents. Buffered 80 % aqueous acetone gave an error of 2.1 % for Chl a and 7.1 % for Chl b, while DMF gave errors of 3.5 and 6.7 %, respectively. For current Chl determination we propose a non-abrasive method, which extracts 15 leaf discs (diameter 6 mm) with 10 cm3 of buffered 80 % aqueous acetone or DMF in hermetically sealed flasks during 24 h in darkness at 4 °C.

Additional key words: Chamaemelum nobile; photochemical efficiency; photoinhibition; photosystem 2; Tuberaria guttata

Prepublished online: December 1, 1998; Published: January 1, 1998  Show citation

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García, A.L., & Nicolás, N. (1998). Influence of the Degree of Solvent Impurity on the Spectrophotometric Determination of Chlorophylls in 80 % Aqueous Acetone and Dimethyl formamide. Application to Non-Abrasive Extraction of Leaves of Citrus Aurantium. Photosynthetica, 35(4), 545-550. doi: 10.1023/A:1006978907364
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