Photosynthetica 1999, 36(1):21 | DOI: 10.1023/A:1007006401360

The Influence of Increased Solar UV-B Radiation on Magnesium-Deficient Cowpea Seedlings: Changes in the Photosynthetic Characteristics

A. Premkumar1, G. Kulandaivelu1
1 School of Biological Sciences, Madurai Kamaraj University, Madurai, India

The influence of increased solar UV-B radiation on the photosynthetic characteristics in cowpea seedlings (Vigna unguiculata) grown at optimal (Mgs) and low (Mgd) Mg levels were studied. Both higher UV-B and Mgd treatments caused significant drops of photochemical activities and net CO2 uptake rates (PN). Yet the UV-B-induced decrease in the photosynthetic efficiency was lesser in Mgd seedlings. The leaf Chl a fluorescence measurements proved that after receiving an enhanced UV-B radiation these seedlings showed a significant enhancement in their variable parts. The PSM oscillation of slow fluorescence kinetics was remarkably altered by both treatments. The PN also followed a typical inhibitory pattern as seen in photochemical activities. Concentrations of several chloroplast proteins in trifoliate leaves were significantly reduced by Mgd treatment and unaffected by the other two treatments. Whereas the contents of 43-47 kDa polypeptides in primary leaves were markedly reduced with a maximal effect in Mgd seedlings, no major difference was noted for combined stress.

Additional key words: chlorophyll; chlorophyll fluorescence induction; chloroplast proteins; CO2 uptake; net photosynthetic rate; photochemical activities; Vigna unguiculata

Published: June 1, 1999  Show citation

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Premkumar, A., & Kulandaivelu, G. (1999). The Influence of Increased Solar UV-B Radiation on Magnesium-Deficient Cowpea Seedlings: Changes in the Photosynthetic Characteristics. Photosynthetica, 36(1-2), 21. doi: 10.1023/A:1007006401360
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    References

    1. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris.-Plant Physiol. 24: 1-15, 1949. Go to original source...
    2. Ballare, C.L., Scopel, A.L., Stapleton, A.E., Yanovsky, M.J.: Solar ultraviolet-B radiation affects seedling emergence, DNA integrity, plant morphology, growth rate and attactiveness to herbivore insects in Datura ferox.-Plant Physiol. 112: 161-170, 1996. Go to original source...
    3. Bornman, J.F.: Taraget sites of UV-B radiation in photosynthesis of higher plants.-J. Photochem. Photobiol. B 4: 145-158, 1989. Go to original source...
    4. Brennan, R.F.: Effect of nitrogen and phosphorus deficiency in wheat on the infection of roots by Gaeumannomyces graminis var. tritici.-Aust. J. agr. Res. 40: 489-495, 1989. Go to original source...
    5. Caldwell, C.R.: The modification of the cellular heat sensitivity of cucumber by growth under supplemental ultraviolet-B radiation.-Plant Physiol. 104: 395-399, 1994. Go to original source...
    6. Deckmyn, G., Martens, C., Impens, I.: The importance of the ratio UV-B/photosynthetic active radiation (PAR) during leaf development as determining factor of plant sensitivity to increased UV-B irradiance: effects on growth, gas exchange and pigmentation of bean plants (Phaseolus vulgaris cv. Label).-Plant Cell Environ. 17: 295-301, 1994. Go to original source...
    7. Drilias, P., Karabourniotis, G., Levizou, E., Nikolopoulos, D., Petropopoulou, Y., Manetas, Y.: The effects of enhanced UV-B radiation on the Mediterranean evergreen sclerophyll Nerium oleander depend on the extent of summer precipitation.-Aust. J. Plant Physiol. 24: 301-306, 1997. Go to original source...
    8. Fiscus, E.L., Booker, F.L.: Is increased UV-B a threat to photosynthesis and productivity?-Photosynth. Res. 43: 81-92, 1995. Go to original source...
    9. Friso, G., Vass, I., Spetea, C., Barber, J., Barbato, R.: UV-B induced degradation of the D1 protein in isolated reaction centre of photosystem II.-Biochim. biophys. Acta 1231: 41-46, 1995. Go to original source...
    10. Godde, D., Hefer, M.: Photoinhibition and light-dependent turnover of the D1 reaction-centre polypeptide of photosystem II are enhanced by mineral-stress conditions.-Planta 193: 290-299, 1994. Go to original source...
    11. Gonzalez, R., Paul, N.D., Percy, K., Ambrose, M., Mclaughlin, C.K., Barnes, J.D., Areses, M., Weliburn, A.R.: Responses to ultraviolet-B radiation (280-315 nm) of pea (Pisum sativum) lines differing in leaf surface wax.-Physiol. Plant. 98: 852-860, 1997. Go to original source...
    12. Grant-Petersson, Renwick, J.A.A.: Effects of ultraviolet-B exposure of Arabidopsis thaliana on herbivory by two crucifer feeding insects (Lepidoptera).-Environ. Entomol. 25: 135-142, 1996. Go to original source...
    13. Joslin, J.D., Wolfe, M.H.: Foliar deficiencies of mature Southern Appalachian red spruce determined from fertilizer trials.-Soil Sci. Amer. Proc. 58: 1572-1579, 1994. Go to original source...
    14. Kulandaivelu, G., Lingakumar, K., Premkumar, S.: UV-B Radiation.-In: Prasad, M.N.V. (ed.): Plant Ecophysiology. Pp. 41-60. John Wiley and Sons, New York 1997.
    15. Laemmli, U.K.: Cleavage of structural proteins during the assembiy of the head of bacteriophage T4.-Nature 227: 680-685, 1970. Go to original source...
    16. Lingakumar, K., Kulandaivelu, G.: Changes induced by ultraviolet-B radiation in vegetative growth, foliar characteristics and photosynthetic activities in Vigna uniguiculata.-Aust. J. Plant Physiol. 20: 299-308, 1993. Go to original source...
    17. Lowry, O.H., Roscbrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
    18. Marschner, H.: Functions of mineral nutrients.-In: Mineral Nutrition of Higher Plants. Pp. 195-340. Academic Press, London 1986.
    19. Marschner, H., Cakmak, I.: High light intensity enhanced chlorosis and necrosis in leaves of zinc, potassium, and magnesium deficient bean (Phaseolus vulgaris) plants.-J. Plant Physiol. 134: 308-315, 1989. Go to original source...
    20. McSwain, B.D., Tsujimoto, H.Y., Arnon, D.I.: Effects of magnesium and chloride ions on light-induced electron transport in membrane fragments from a blue-green alga.-Biochim. biophys. Acta 423: 313-322, 1976. Go to original source...
    21. Melis, A., Nemson, J.A., Harrison, M.A.: Damage to functional components and partial degradation of photosystem II reaction center proteins upon chloroplast exposure to ultraviolet-B radiation.-Biochim. biophys. Acta 1100: 312-320, 1992. Go to original source...
    22. Mulo, P., Tyystijärvi, T., Tyystjärvi, E., Govindjee, Maenpaa, P., Aro, E.M.: Mutagensis of the D-E loop of photosystem II centre protein D1. Function and assembly of photosystem II.-Plant mol. Biol. 33: 1059-1071, 1997. Go to original source...
    23. Musil, C.F., Wand, J.E.: Differential stimulation of an arid-environment winter ephemeral Dimorphotheca pluvialis (L.) Moench by ultraviolet-B radiation under nutrient limitation.-Plant Cell Environ. 17: 245-255, 1994. Go to original source...
    24. Naidu, S.L., Sullivan, J.H., Teramura, A.H., DeLucia, E.H.: The effects of ultraviolet-B radiation on photosynthesis of different aged needles in field-grown loblolly pine.-Tree Physiol. 12: 151-162, 1993. Go to original source...
    25. Nedunchezhian, N., Kulandaivelu, G.: Evidence for the ultraviolet-B (280-320 nm) radiation induced structural reorganization and damage of photosystem II polypeptides in isolated chloroplasts.-Physiol. Plant. 81: 558-562, 1991. Go to original source...
    26. Nedunchezhian, N., Kulandaivelu, G.: Changes induced by ultraviolet-B (280-320 nm) radiation to vegetative growth and photosynthetic characteristics in field grown Vigna unguiculata L.-Plant Sci. 123: 85-92, 1997. Go to original source...
    27. Nedunchezhian, N., Ravindran, K.C., Kulandaivelu, G.: Changes in photosynthetic apparatus during dark incubation of detached leaves from control and ultraviolet-B treated Vigna seedlings.-Biol. Plant. 37: 341-348, 1995. Go to original source...
    28. Premkumar, A., Kulandaivelu, G.: Influence of ultraviolet-B enhanced solar radiation on growth and photosynthesis of potassium deficient cowpea seedlings.-Photosynthetica 32: 521-528, 1996.
    29. Premkumar, A., Nedunchezhian, N., Kulandaivelu, G.: Growth and photosynthesis of mulberry under ultraviolet-B enhanced solar irradiances.-Sericologia 36: 645-649, 1996.
    30. Reeves, S.G., Hall, D.O.: The stoichiometry (ATP/2e- ratio) on non-cyclic photophosphorylation in isolated spinach chloroplasts.-Biochim. biophys. Acta 314: 66-78, 1973. Go to original source...
    31. Rintamaki, E., Salo, R., Koivuniemi, A., Aro, E.M.: Protein phosphorylation and magnesium status regulate the degradation of the D1 reaction centre protein of photosystem II.-Plant Sci. 115: 175-182, 1996. Go to original source...
    32. Suichu, Q., Jun, L., Qizhi, L.: Investigation on Mg deficiency in wheat.-J. Zhejiang agr. Sci. 6: 273-275, 1995.
    33. Terry, N., Ulrich, A.: Effects of magnesium deficiency on the photosynthesis and respiration of leaves of sugar beet.-Plant Physiol. 54: 379-381, 1974. Go to original source...
    34. Vass, I., Sass, L., Spetea, C., Bakou, A., Ghanotakis, D.F., Petrouleas, V.: UV-B induced inhibition of photosystem II electron transport studied by EPR and chlorophyll fluorescence. Impairment of donor and acceptor side components.-Biochemistry 35: 8964-8973, 1996. Go to original source...
    35. Visser, A.J., Tosserams, M., Groen, M.W., Magendans, G.W.H., Rozema, J.: The combined effects of CO2 concentration and solar UV-B radiation on faba bean grown in open-top chambers.-Plant Cell Environ. 20: 189-199, 1997. Go to original source...
    36. Wilson, M.I., Greenberg, B.M.: Specificity and photomorphogenic nature of ultraviolet-B induced cotyledon curling in Brassica napus L.-Plant Physiol. 102: 671-677, 1993. Go to original source...
    37. Zeuthen, J., Mikkelsen, T.N., Paludan-Müller, G., Ro-Poulsen, H.: Effects of increased UV-B radiation and elevated levels of tropospheric ozone on physiological processes in European beach (Fagus sylvatica).-Physiol. Plant. 100: 281-290, 1997. Go to original source...

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