Photosynthetica 1999, 36(4):557-563 | DOI: 10.1023/A:1007044121420

Effects of Proline and Betaine on Heat Inactivation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Crude Extracts of Rice Seedlings

M.L. Dionisio-Sese1, M. Shono1, S. Tobita1
1 Okinawa Subtropical Station, Japan International Research Center for Agricultural Sciences (JIRCAS), Ishigaki, Okinawa, Japan

Activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (RuBPCO) in heated crude extracts from seedlings of the rice cultivars Hitomebore and IR28 was investigated in the presence of proline and betaine. Both solutes retarded the inactivation of the enzyme extracted from the leaves of both cultivars at temperature-stress from 35 to 45 °C. At 50 °C, however, betaine was effective in both cultivars. Stabilization of RuBPCO activity was independent of the added solute from 1 to 2 M concentration.

Additional key words: cultivar differences; Oryza sativa L

Prepublished online: January 1, 2000; Published: December 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Dionisio-Sese, M.L., Shono, M., & Tobita, S. (1999). Effects of Proline and Betaine on Heat Inactivation of Ribulose-1,5-Bisphosphate Carboxylase/Oxygenase in Crude Extracts of Rice Seedlings. Photosynthetica, 36(4), 557-563. doi: 10.1023/A:1007044121420
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Arakawa, T., Timasheff, S.N.: The stabilization of protein by osmolytes.-Biophys. J. 47: 411-414, 1985. Go to original source...
    2. Bose, A., Ghosh, B.: Effect of heat stress on ribulose 1,5-bisphosphate carboxylase in rice.-Phytochemistry 38: 1115-1118, 1995. Go to original source...
    3. Chu, T.M., Aspinall, D., Paleg, L.G.: Stress metabolism. VI. Temperature stress and the accumulation of proline in barley and radish.-Aust. J. Plant Physiol. 1: 87-97, 1974. Go to original source...
    4. Demmig, B., Winter K.: Sodium, potassium, chloride and proline concentrations of chloroplasts isolated from a halophyte, Mesembryanthemum crystallinum L.-Planta 168: 421-426, 1986. Go to original source...
    5. Greenway, H., Munns, R.: Mechanisms of salt tolerance in nonhalophytes.-Annu. Rev. Plant Physiol. 31: 149-190, 1980. Go to original source...
    6. Hanson, A.D., May, A.M., Grumet, R., Bode, J., Jamieson, G.C., Rhodes, D.: Betaine synthesis in chenopods: localization in chloroplasts.-Proc. nat. Acad. Sci. USA 82: 3678-3682, 1985. Go to original source...
    7. Hanson, A.D., Nelsen, C.E.: Betaine accumulation and [14C]formate metabolism in water-stressed barley leaves.-Plant Physiol. 62: 305-312, 1978. Go to original source...
    8. Hayashi, Y., Shono, M., Tsutenomi, N., Tanaka, A., Nomura, M., Takabe, T., Takabe, T.: Glycine betaine-producing rice plants had enhanced tolerance to salt stress.-Plant Physiol. 114(Suppl.): 120, 1997.
    9. Jolivet, Y., Larher, F., Hamelin, J.: Osmoregulation in halophytic higher plants: the protective effect of glycine betaine against the heat destabilization of membranes.-Plant Sci. Lett. 25: 193-201, 1982. Go to original source...
    10. Kuo, C.G., Chen, H.M., Ma, L.H.: Effect of high temperature on proline content in tomato floral buds and leaves.-J. amer. Soc. hort. Sci. 111: 746-750, 1986. Go to original source...
    11. Lan, Y., Mott, K.A.: Determination of apparent Km values for ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco) activase using the spectrophotometric assay of Rubisco activity.-Plant Physiol. 95: 604-609, 1991. Go to original source...
    12. Laurie, S., Stewart, G.R.: The effects of compatible solutes on the heat stability of glutamine synthetase from chickpeas grown under different nitrogen and temperature regimes.-J. exp. Bot. 41: 1415-1422, 1990. Go to original source...
    13. Manetas, Y., Petropolou, Y., Karabourniotis, G.: Compatible solutes and their effects on phosphoenolpyruvate carboxylase of C4-halophytes.-Plant Cell Environ. 9: 145-151, 1986. Go to original source...
    14. Naidu, B.P., Paleg, L.G., Aspinall, D., Jennings, A.C., Jones, G.P.: Amino acid and glycine betaine accumulation in cold-stressed wheat seedlings.-Phytochemistry 30: 407-409, 1991. Go to original source...
    15. Nash, D., Paleg, L.G., Wiskich, J.T.: Effect of proline, betaine and some other solutes on the heat stability of mitochondrial enzymes.-Aust. J. Plant Physiol. 9: 47-57, 1982. Go to original source...
    16. Nikolopoulos, D., Manetas, Y.: Compatible solutes and in vitro stability of Salsoda soda enzymes: proline incompatibility.-Phytochemistry 30: 411-413, 1991. Go to original source...
    17. Paleg, L.G., Douglas, T.J., Van Daal, A., Keech, D.B.: Proline, betaine and other organic solutes protect enzymes against heat inactivation.-Aust. J. Plant Physiol. 8: 107-114, 1981. Go to original source...
    18. Papageorgiou, G.C., Murata, N.: The unusually strong stabilizing effects of glycine betaine on the structure and function of the oxygen-evolving Photosystem II complex.-Photosynth. Res. 44: 243-252, 1995. Go to original source...
    19. Pollard, A., Wyn Jones, R.G.: Enzyme activities in concentrated solutions of glycinebetaine and other solutes.-Planta 144: 291-298, 1979. Go to original source...
    20. Rajasekaran, L.R., Kriedemann, P.E., Aspinall, D., Paleg, L.G.: Physiological significance of proline and glycinebetaine: Maintaining photosynthesis during NaCl stress in wheat.-Photosynthetica 34: 357-366, 1997. Go to original source...
    21. Rathinasabapathi, B., Gage, D., Mackill, D., Hanson, A.: Cultivated and wild rices do not accumulate glycine-betaine due to deficiencies in two biosynthetic steps.-Crop Sci. 33: 534-538, 1993. Go to original source...
    22. Roy, D., Bhunia, A., Basu, N., Barnejee, S.K.: Effect of NaCl-salinity on metabolism of proline in salt-sensitive and salt-resistant cultivars of rice.-Biol. Plant. 34: 159-162, 1992. Go to original source...
    23. Shomer-Ilan, A., Jones, G.P., Paleg, L.G.: In vitro thermal and salt stability of pyruvate kinase are increased by proline analogues and trigonelline.-Aust. J. Plant Physiol. 18: 279-286, 1991. Go to original source...
    24. Shomer-Ilan, A., Waisel, Y.: Effects of stabilizing solutes on salt activation of phosphoenolpyruvate carboxylase from various plant sources.-Physiol. Plant. 67: 408-414, 1986. Go to original source...
    25. Singh, T.N., Aspinall, D., Paleg, L.G.: Proline accumulation and varietal adaptability to drought in barley: a potential metabolic measure of drought resistance.-Nature new Biol. 236: 188-190, 1972. Go to original source...
    26. Stamatakis, K., Gavalas, N.A., Manetas, Y.: Organic cosolutes increase the catalytic efficiency of phosphoenolpyruvate carboxylase, from Cynodon dactylon (L.) Pers., apparently through self-association of the enzymic protein.-Aust. J. Plant Physiol. 15: 621-631, 1988. Go to original source...
    27. Storey, R., Ahmad, N., Wyn Jones, R.G.: Taxonomic and ecological aspects of the distribution of glycinebetaine and related compounds in plants.-Oecologia 27: 319-332, 1977. Go to original source...
    28. Storey, R., Wyn Jones, R.G.: Betaine and choline levels in plants and their relationship to NaCl stress.-Plant Sci. Lett. 4: 161-168, 1975. Go to original source...
    29. Storey, R., Wyn Jones, R.G.: Quarternary ammonium compounds in plants in relation to salt resistance.-Phytochemistry 16: 447-453, 1977. Go to original source...
    30. Velitchkova, M., Fedina, I.: Response of photosynthesis of Pisum sativum to salt stress as affected by methyl jasmonate.-Photosynthetica 35: 89-97, 1998. Go to original source...
    31. Wyn Jones, R.G., Storey, R.: Salt stress and comparative physiology in the Gramineae. II. Glycinebetaine and proline accumulation in two salt-and water-stressed barley cultivars.-Aust. J. Plant Physiol. 5: 817-829, 1978. Go to original source...

    Return to the content