Photosynthetica 1999, 36(11):53-59 | DOI: 10.1023/A:1007066812957

Developmental and Photosynthetic Characteristics of a Photoautotrophic Chrysanthemum Culture

V. Cristea1, F. Dalla Vecchia2, N. La Rocca2
1 Biological Research Institute, Cluj-Napoca, Romania
2 Department of Biology, University of Padova, Padova, Italy

Chrysanthemum plantlets were cultivated in vitro on media with 2.0, 0.3, or 0 % sucrose, or photoautotrophically without an organic carbon source but with supplementation of the culture vessel atmosphere with 2 % CO2. The photoautotrophically cultivated plantlets showed a better growth and multiplication, higher contents of chlorophyll (Chl) and carotenoids, higher Chl a/b ratio, net photosynthetic rate and ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activities than plantlets grown on the medium with sucrose.

Additional key words: carotenoids; chlorophyll; in vitro CO2 supply; net photosynthetic rate; phosphoenolpyruvate carboxylase; photosynthetic pigments; ribulose-1,5-bisphosphate carboxylase; suncaps

Published: July 1, 1999  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Cristea, V., Dalla Vecchia, F., & La Rocca, N. (1999). Developmental and Photosynthetic Characteristics of a Photoautotrophic Chrysanthemum Culture. Photosynthetica37(1), 53-59. doi: 10.1023/A:1007066812957
Download citation

References

  1. Cristea, V., Dalla Vecchia, F., CrĂciun, C.: Biochemical and ultrastructural aspects of carrot (Daucus carota L.) tissue culture in different experimental growth conditions.-G. bot. ital. 130: 924-926, 1996. Go to original source...
  2. Cristea, V., Dalla Vecchia, F., CrĂciun, C., La Rocca, N.: Ultrastructural aspects of photoautotrophic Chrysanthemum culture.-In: Garáb, G. (ed.): Photosynthesis: Mechanisms and Effects. Pp. 4175-4178. Kluwer Academic Publ., Dordrecht-Boston-London 1999. Go to original source...
  3. Debergh, P.C., De Meester, J., De Riek, J., Gillis, S., Van Huylenbroeck, J.: Ecological and physiological aspects of tissue-cultured plants.-Acta bot. neerl. 41: 417-423, 1992. Go to original source...
  4. Desjardins, Y., Hdider, C., De Riek, J.: Carbon nutrition in vitro - regulation and manipulation of carbon assimilation in micropropagated systems.-In: Aitken-Christie, J., Kozai, T., Smith, M.A.L. (ed.): Automation and Environmental Control in Plant Tissue Culture. Pp. 441-471. Kluwer Academic Publ., Dordrecht-Boston-London 1995. Go to original source...
  5. Di Marco, G., Tricoli, D.: RuBP carboxylase determination by enzymic estimation of D-3-PGA formed.-Photosynth. Res. 4: 145-149, 1983. Go to original source...
  6. Fujiwara, K., Kira, S., Kozai, T.: Time course of CO2 exchange of potato cultures in vitro with different sucrose concentrations in the culture medium.-J. agr. Meteorol. 48: 49-56, 1992. Go to original source...
  7. Fujiwara, K., Kozai, T., Watanabe, I.: Development of a photoautotrophic tissue culture system for shoots and/or plantlets at rooting and acclimatization stages.-Acta Hort. 230: 153-158, 1988. Go to original source...
  8. Goldstein, C.S., Widholm, J.M.: Photosynthetic characterization of photoautotrophic cells cultured in a minimal medium.-Plant Physiol. 94: 1641-1646, 1990. Go to original source...
  9. Hdider, C., Desjardins, Y.: Changes in ribulose-1,5-bisphosphate carboxylase/oxygenase and phosphoenolpyruvate carboxylase activities and 14CO2 fixation during the rooting of strawberry shoots in vitro.-Can. J. Plant. Sci. 74: 827-831, 1994a. Go to original source...
  10. Hidider, C., Desjardins, Y.: Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets.-Plant Cell Tissue Organ Cult. 36: 27-33, 1994b. Go to original source...
  11. Hüsemann, W.: Establishment of photoautotrophic cell cultures.-In: Plant Tissue Culture Manual H1. Pp. 1-30. Kluwer Academic Publ., Dordrecht 1995. Go to original source...
  12. Ishii, R., Yamagishi, T., Murata, Y.: On a method for measuring photosynthesis and respiration of leaf slices with an oxygen electrode.-Jap. J. Crop Sci. 46: 53-57, 1977. Go to original source...
  13. Kozai, T.: Micropropagation under photoautotrophic conditions.-In: Deberg, P.C., Zimmerman, R.H. (ed.): Micropropagation. Technology and Application. Pp. 447-469. Kluwer Academic Publ., Dordrecht-Boston-London 1991. Go to original source...
  14. Kozai, T., Fujiwara, K., Hayashi, M., Aitken-Christie, J.: The in vitro environment and its control in micropropagation.-In: Kurata, K., Kozai, T. (ed.): Transplant Production Systems. Pp. 247-282. Kluwer Academic Publ., Dordrecht-Boston-London 1992. Go to original source...
  15. Kozai, T., Kitaya, Y., Fujiwara, K., Adelberg, J.: Environmental control for large scale production of in vitro plantlets.-In: Terzi, M., Cella, R., Falavigna, A. (ed.): Current Issues in Plant Molecular and Cellular Biology. Pp. 659-667. Kluwer Academic Publishers, Dordrecht 1995. Go to original source...
  16. Langford, P.J., Wainwright, H.: Effects of sucrose concentration on the photosynthetic ability of rose shoots in vitro.-Ann. Bot. 60: 633-640, 1987. Go to original source...
  17. Murashige, T., Skoog, F.: A revised medium for rapid growth and bioassays with tobacco tissue cultures.-Physiol. Plant. 18: 473-497, 1962. Go to original source...
  18. Pospíšilová, J., Čatský, J., Šesták, Z.: Photosynthesis in plant cultivated in vitro.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525-540. Marcel Dekker, New York-Basel-Hong Kong 1997.
  19. Pospíšilová, J., Čatský, J., Solárová, J., Tichá, I.: Photosynthesis of plant regenerants. Specificity of in vitro conditions and plantlet response.-Biol. Plant. 29: 415-421, 1987. Go to original source...
  20. Shimada, N., Tanaka, F., Kozai, T.: Effects of low O2 concentration on net photosynthesis of C3 plantlets in vitro.-Acta Hort. 230: 171-175, 1988. Go to original source...
  21. Solárová, J., Pospíšilová, J.: Effect of carbon dioxide enrichment during in vitro cultivation and acclimation to ex vitro conditions.-Biol. Plant. 39: 23-30, 1997. Go to original source...
  22. Tanaka, F., Watanabe, Y., Shimada, N.: [Effect of O2 concentrations on photorespiration in Chrysanthemum morifolium plantlets in vitro cultured photoautotrophically and photomixotrophically.]-Plant Tissue Cult. Lett. 8: 87-93, 1991a. [In Jap.] Go to original source...
  23. Tanaka, F., Watanabe, Y., Shimada, N.: [Effect of O2 concentration on growth of Chrysanthemum morifolium plantlets in vitro cultured photoautotrophically and photomixotrophically.]-Environ. Control Biol. 29: 107-116, 1991b. [In Jap.] Go to original source...
  24. Tichá, I.: Optimization of photoautotrophic tobacco in vitro culture: effect of suncaps closures on plantlet growth.-Photosynthetica 32: 475-479, 1996.
  25. Tichá, I., Ondřej, M., Hrouda, M., Čatský, J., Zima, J., Pospíšilová, J., Solárová, J.: Photosynthesis in regenerants of tobacco transformed by plasmids of Agrobacterium I. Morphology, anatomy and gas exchange of transformants.-Photosynthetica 22: 350-363, 1988.
  26. Usuda, H., Ku, M.S.B., Edwards, G.E.: Activation of NADP-malate dehydrogenase, pyruvate, Pi dikinase, and fructose 1,6-bisphosphatase in relation to photosynthetic rate in maize.-Plant Physiol. 76: 238-243, 1984. Go to original source...
  27. Van Huylenbroeck, J.M., Deberg, C.P.: Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets.-Physiol. Plant. 96: 298-304, 1996. Go to original source...
  28. Van Huylenbroeck, J.M., De Riek, J.: Sugar and starch metabolism during ex vitro rooting and acclimatization of micropropagated Spathiphyllum 'Petite' plantlets.-Plant Sci. 111: 19-25, 1995. Go to original source...
  29. Wellburn, A. R.: The spectral determination of chlorophylls a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution.-J. Plant. Physiol. 144: 307-313, 1994. Go to original source...
  30. Widholm, J.M.: Properties and uses of photoautotrophic plant cell cultures.-Int. Rev. Cytol. 132: 109-175, 1992. Go to original source...
  31. Widholm, J.M.: Photosynthetic plant cell cultures: an overview.-In: Carre, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in Vitro Cultures. Saint-Paul-lez-Durance, France. Pp. 15-24. CEA, Cadarache 1995.