Photosynthetica 1999, 36(12):183-200 | DOI: 10.1023/A:1007047703388

Integrity and Activity of Photosystem 2 Complexes Isolated from the Thermophilic Cyanobacterium Synechococcus Elongatus Using Various Detergents

E. Šetlíková1,3, D. Sofrová2, O. Prášil1,3, P. Budáč1,3, M. Koblížek1,3, I. Šetlík1,3
1 Department of Autotrophic Microorganisms, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
2 Department of Biochemistry, Faculty of Science, Charles University, Praha, Czech Republic
3 Biological Faculty, University of South Bohemia, České Budějovice, Czech Republic

The efficiency in selective extraction of photosystem (PS) 2 oxygen evolving complexes was compared among seven detergents. These were applied to thylakoid membranes of the thermophilic cyanobacterium Synechococcus elongatus. Used were five non-ionic detergents with one ionic and one zwitterionic for comparison. To compare the suitability and efficiency of the detergents the following properties of the extracts were examined: maximum rate of oxygen evolution with various electron acceptors, the relative variable fluorescence (FV/FM), the contamination of the extract with photosystem (PS) 1, and the status of the electron acceptor side of PS2 reaction centre. None of the detergents yielded a highly selective extraction of the PS2 complexes (negligible contamination with PS1) which would simultaneously display a high photochemical activity and high structural intactness. Heptylthioglucoside and dodecylmaltoside yielded the nearest approximation to the optimum result. Kinetic fluorometry was applied here for the first time to characterize the functional and structural properties of PS2 particles from cyanobacteria.

Additional key words: fluorescence induction; low temperature fluorescence spectra; oxygen evolution; PQ pool; QA-reoxidation; QB pocket; thermoluminescence

Published: September 1, 1999  Show citation

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Šetlíková, E., Sofrová, D., Prášil, O., Budáč, P., Koblížek, M., & Šetlík, I. (1999). Integrity and Activity of Photosystem 2 Complexes Isolated from the Thermophilic Cyanobacterium Synechococcus Elongatus Using Various Detergents. Photosynthetica, 37(2), 183-200. doi: 10.1023/A:1007047703388
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