Photosynthetica 1999, 36(12):249-254 | DOI: 10.1023/A:1007160106113

Measurements of Phytoplankton of Sub-Nanomolar Chlorophyll Concentrations by a Modified Double-Modulation Fluorometer

N. Dijkman1,2, D. Kaftan1,3, M. Trtílek4, L. Nedbal1
1 NRC Photosynthesis & Global Climate Change, Institute of Microbiology, Academy of Sciences of the Czech Republic, Třeboň, Czech Republic
2 Alfred Wegener Institute for Polar and Marine Research, Bio-Optics, Bremerhaven, Germany
3 Faculty of Biology, University of South Bohemia, České Budějovice, Czech Republic
4 P.S. Instruments, Brno, Czech Republic

A modification of the double-modulation fluorometer is described that allows measuring very dilute phytoplankton samples. The high sensitivity is achieved by increasing the sample volume and by collecting the fluorescence from the large volume by an integrating sphere. The sensitivity of the instrument increased approximately proportionally to the volume of the sample. A further improvement of the sensitivity was achieved by replacing the PIN photodiode of the earlier versions by a photomultiplier. The instrument was used to measure fluorescence induction, F0 and Fm parameters, and QA -reoxidation kinetics at concentrations at and below 100 pM chlorophyll.

Additional key words: fluorescence; Phaeodactylum tricornutum; Pseudococcomyxa sp.; Synechococcus marinus

Published: September 1, 1999  Show citation

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Dijkman, N., Kaftan, D., Trtílek, M., & Nedbal, L. (1999). Measurements of Phytoplankton of Sub-Nanomolar Chlorophyll Concentrations by a Modified Double-Modulation Fluorometer. Photosynthetica, 37(2), 249-254. doi: 10.1023/A:1007160106113
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