Photosynthetica 1999, 36(14):553-558 | DOI: 10.1023/A:1007115307551

Inhibition of Pigment Biosynthesis in Cucumber Cotyledons by Isoxaflutole

S. Kushwaha1, P.C. Bhowmik1
1 Department of Plant and Soil Sciences, University of Massachusetts, Amherst, U.S.A

Isoxaflutole [5-cyclopropyl-4-(2-methylsulphonyl-4-trifluromethylbenzoyl)isoxazole] is a new preemergence herbicide for broad-spectrum weed control in maize. The effect of isoxaflutole on chlorophyll (Chl) and carotenoid (Car) biosynthesis was investigated in cucumber (Cucumis sativus L.) cotyledons. Etiolated tissue was incubated with 5 mM isoxaflutole for 24 h and irradiated (60 μmol m-2 s-1). The irradiation for 3 h did not reduce Chl a, Chl b, and Car contents, but after a 28-h irradiation the contents of Chl a and Car decreased by 35 and 15 %, respectively, and the content of Chl b increased by 24 %. Increasing the concentration of isoxaflutole beyond 5 mM resulted in reduction of Chl a (71 %), Chl b (20 %), and Car (31 %) contents. Similarly, increase in irradiance from 60 to 180 μmol m-2 s-1 resulted in larger reduction of Chl and Car contents. Exogenously supplied 5-aminolevulinic acid did not reverse the isoxaflutole-inhibited Chl synthesis, whereas an exogenously supplied homogentisic acid lactone reversed the inhibition of pigment synthesis due to isoxaflutole.

Additional key words: 5-aminolevulinic acid; Cucumis sativus; homogentisic acid; hydroxyphenyl pyruvate dioxygenase

Prepublished online: December 1, 2000; Published: December 31, 1999  Show citation

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Kushwaha, S., & Bhowmik, P.C. (1999). Inhibition of Pigment Biosynthesis in Cucumber Cotyledons by Isoxaflutole. Photosynthetica, 37(4), 553-558. doi: 10.1023/A:1007115307551
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