Photosynthetica 2000, 38(1):23-28 | DOI: 10.1023/A:1026783521733

Modulation of Phosphoenolpyruvate Carboxylase Phosphorylation in Leaves of Amaranthus hypochondriacus, a NAD-ME Type of C4 Plant

K. Parvathi1, J. Gayathri1, G.B. Maralihalli2, A.S. Bhagwat2, A.S. Raghavendra3
1 Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India
2 Molecular Biology and Agriculture Division, Bhabha Atomic Research Center, Trombay, Mumbai, India
3 Department of Plant Sciences, School of Life Sciences, University of Hyderabad, Hyderabad, India

PEP carboxylase (PEPC) in leaves of C4 plants is activated by phosphorylation of enzyme by a PEPC-protein kinase (PEPC-PK). We reevaluated the pattern of PEPC phosphorylation in leaf extracts of Amaranthus hypochondriacus. It was dependent on Ca2+, the optimum concentration of which for stimulation was 10 mM. The extent of stimulation was inhibited by 1,2-bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic acid (BAPTA), a Ca2+ chelator. The inhibition by BAPTA was relieved by the addition of Ca2+ but not by the addition of Mg2+. The stimulation by Ca2+ of PEPC phosphorylation was marginally enhanced by calmodulin (CaM), but not by diacylglycerol (DAG). Phosphorylation was strongly restricted by Ca2+ or Ca2+-CaM-dependent protein kinase inhibitors. Thus phosphorylation of PEPC is Ca2+-dependent in leaves of A. hypochondriacus and a calcium-dependent protein kinase (CDPK) may modulate PEPC-PK and subsequently the phosphorylation status of PEPC.

Additional key words: calcium; calcium-dependent protein kinase; PEPC-protein kinase

Prepublished online: October 1, 2000; Published: April 1, 2000  Show citation

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Parvathi, K., Gayathri, J., Maralihalli, G.B., Bhagwat, A.S., & Raghavendra, A.S. (2000). Modulation of Phosphoenolpyruvate Carboxylase Phosphorylation in Leaves of Amaranthus hypochondriacus, a NAD-ME Type of C4 Plant. Photosynthetica, 38(1), 23-28. doi: 10.1023/A:1026783521733
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