Photosynthetica 2000, 38(1):149-154 | DOI: 10.1023/A:1026716529002

Foliar Spraying with Zineb Increases Fruit Productivity and Alleviates Oxidative Stress in Two Tomato Cultivars

Á. Calatayud1, E. Barreno2
1 Institut Cavanilles de Biodiversitat i Biología Evolutiva, Facultat de Ciències Biológiques, Universitat de València, Burjassot (València), Spain
2 Institut Cavanilles de Biodiversitat i Biología Evolutiva, Facultat de Ciències Biológiques, Universitat de València, Burjassot (València), Spain

The effects of foliar spraying of the dithiocarbamate zineb on two cultivars of tomato grown in the field in a site with high ozone concentrations were studied by means of biomass assessment, antioxidant enzyme assays, lipid peroxidation, and chlorophyll fluorescence measurements. Zineb prevented the peroxidation of membrane lipids and decreased the activity of scavenging enzymes, which suggests that plants sprayed with zineb are subjected to lower oxidative stress than controls. The beneficial effects of zineb protection is the utilization of a larger fraction of absorbed radiant energy in photosynthesis and a larger fruit yield in plants of both cultivars.

Additional key words: antioxidant enzymes; chlorophyll fluorescence; lipid peroxidation; Lycopersicon esculentum; ozone; production

Prepublished online: October 1, 2000; Published: April 1, 2000  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Calatayud, Á., & Barreno, E. (2000). Foliar Spraying with Zineb Increases Fruit Productivity and Alleviates Oxidative Stress in Two Tomato Cultivars. Photosynthetica38(1), 149-154. doi: 10.1023/A:1026716529002
Download citation

References

  1. Astorino, G., Margani, I., Tripodo, P., Manes, F.: The response of Phaseolus vulgaris L cv Lit to different dosages of the anti-ozonant ethylenediurea (EDU) in relation to chronic treatment with ozone.-Plant Sci. 111: 237-248, 1995. Go to original source...
  2. Beyer, W.F., Jr., Fridovich, I.: Assaying of superoxide dismutase activity: some large consequences of minor changes in conditions.-Anal. Biochem. 161: 559-566, 1987. Go to original source...
  3. Bilger, W., Björkman, O.: Temperature dependence of violaxanthin de-epoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L.-Planta 184: 226-234, 1991. Go to original source...
  4. Camahan, J.E., Jenner, E.L., Wat, E.K.W.: Prevention of ozone injury to plants by a new protectant chemical.-Phytopathologist 68: 1225-1229, 1978. Go to original source...
  5. Cathey, H.M., Heggestad, H.E.: Reduction of ozone damage to Petunia hybrida (Vilm.) by use of growth regulating chemicals and tolerant chemicals.-J. amer. Soc. hort. Sci. 97: 685-700, 1972. Go to original source...
  6. Conklin, P.L., Last, R.L.: Differential accumulation of antioxidant mRNAs in Arabidopsis thaliana exposed to ozone.-Plant Physiol. 109: 203-212, 1995. Go to original source...
  7. Cooley, D.R., Manning, W.J.: Ozone effects on growth and assimilate partitioning in alfalfa, Medicago sativa L.-Environ. Pollut. 49: 19-36, 1988. Go to original source...
  8. Dass, H.C., Weaver, G.M.: Modification of ozone damage to Phaseolus vulgaris by antioxidants, thiols and sulfhydryl reagents.-Can. J. Plant Sci. 48: 569-574, 1968. Go to original source...
  9. Davison, A.W., Barnes, J.D.: Effects of ozone on wild plants.-New Phytol. 139: 135-151, 1998. Go to original source...
  10. Demmig-Adams, B., Adams, W.W., III., Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation.-Physiol. Plant. 98: 253-264, 1996. Go to original source...
  11. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 32: 93-101, 1981. Go to original source...
  12. Egger, R., Schlee, D., Türk, R.: Changes of physiological and biochemical parameters in the lichen Hypogymnia physodes (L.) Nyl. due to the action of air pollutants - a field study.-Phyton 34: 229-242, 1994.
  13. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
  14. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
  15. Kangasjärvi, J., Talvinen, J., Utrianien, M., Karjalainen, R.: Plant defense systems induced by ozone.-Plant Cell Environ. 17: 783-794, 1994. Go to original source...
  16. Kendrick., J.B., Jr., Darley, E. F., Middleton, J.T.: Chemotherapy for oxidant and ozone induced plant damage.-J. Air Water Pollut. 6: 391-402, 1962.
  17. Kendrick, J.B., Jr., Middleton, J.T., Darley, E.F.: Chemical protection of plants from ozonated olefin (smog) injury.-Phytopathology 44: 494-495, 1954.
  18. Kostka-Rick, R., Manning, W.J.: Effects and interactions of ozone and the anti-ozonant EDU at different stages of radish (Raphanus sativus L.) development.-J. exp. Bot. 43: 1621-1631, 1992. Go to original source...
  19. Lefohn, A.S.: Surface Level Ozone Exposures and Their Effects on Vegetation.-Lewis Publ., Chelsea 1992.
  20. Lichtenthaler, H.K.: Vegetation stress: an introduction to the stress concept in plants.-J. Plant Physiol. 148: 4-14, 1996. Go to original source...
  21. Ormrod, D.P., Beckerson, D.W.: Polyamines.-HortScience 21: 1070-1071, 1986. Go to original source...
  22. Osmond, C.B.: What is photoinhibition? Some insights from comparisons of shade and sun plants.-In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. Pp. 1-24. Bios Scientific Publ., Oxford 1994.
  23. Pell, E.J., Temple, P.J., Friend, A.L., Mooney, H.A., Winner, W.E.: Compensation as a plant response to ozone and associated stress: an analysis of ROPIS experiments.-J. Environ. Quality 23: 429-436, 1994. Go to original source...
  24. Price, A., Lucas, P.W., Lea, P.J.: Age dependent damage and glutathione metabolism in ozone fumigated barley: a leaf section approach.-J. exp. Bot. 41: 1309-1317, 1990. Go to original source...
  25. Ranieri, A., D'Urso, G., Nali, C., Lorenzini, G., Soldatini, G.F.: Ozone stimulates apoplastic antioxidant systems in pumpkin leaves.-Physiol. Plant. 97: 381-387, 1996. Go to original source...
  26. Rao, M.V.: Cellular detoxifying mechanisms determine the age dependent injury in tropical trees exposed to SO2.-J. Plant Physiol. 140: 733-740, 1992. Go to original source...
  27. Rao, M.V., Ormrod, D.P.: Impact of UVB and O3 on the oxygen free radical system in Arabidopsis thalliana genotypes differing in flavonoid biosynthesis.-Photochem. Photobiol. 62: 719-726, 1995. Go to original source...
  28. Rao, M.V., Gopinadhan, P., Ormrod, D.P.: Ultraviolet-B-and ozone-induced biochemical changes in antioxidant enzymes of Arabidopsis thaliana.-Plant Physiol. 110: 125-136, 1996. Go to original source...
  29. Schreiber, U., Schliwa, U., Bilger, B.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
  30. Sharma, Y.K., Davis, K.R.: Ozone-induced expression of stress-related genes in Arabidopsis thaliana.-Plant Physiol. 105: 1089-1096, 1994. Go to original source...
  31. Sharma, Y.K., León, J., Raskin, I., Davis, K.R.: Ozone-induced responses in Arabidopsis thalliana: The role of salicylic acid in the accumulation of defense-related transcripts and induced resistance.-Proc. nat. Acad. Sci. USA 93: 5099-5104, 1996. Go to original source...
  32. Tomlinson, H., Rich, S.: Relating lipid content and fatty acid synthesis to ozone injury of tobacco leaves.-Phytopathology 59: 1284-1286, 1973.
  33. Yoshida, M., Nouchi, Y., Toyama, S.: Studies on the role of active oxygen in ozone injury to plant cells. I. Generation of active oxygen in rice protoplast exposed to ozone.-Plant Sci. 95: 197-205, 1994. Go to original source...