Photosynthetica 2000, 38(2):281-286 | DOI: 10.1023/A:1007214915785

Chlorophyll a Fluorescence Emission, Xanthophyll Cycle Activity, and Net Photosynthetic Rate Responses to Ozone in Some Foliose and Fruticose Lichen Species

A. Calatayud1, P.J. Temple2, E. Barreno3
1 Department of Plant Biology, Universitat de València, Burjassot (València), Spain
2 PSW Research Experimental Station, U.S. Forest Service, Riverside, USA
3 Department of Plant Biology, Universitat de València, Burjassot (València), Spain

The lichens Parmelia quercina, Parmelia sulcata, Evernia prunastri, Hypogymnia physodes, and Anaptychia ciliaris were exposed to ozone (O3) in controlled environment cuvettes designed to maintain the lichens at optimal physiological activity during exposure. Measurements of gas exchange, modulated chlorophyll (Chl) fluorescence, and pigment analysis were conducted before and after exposure to 300 mm3 (O3) m-3, 4 h per d for 14 d. No changes in the efficiency of photosystem 2 (PS2) photochemistry, the reduction state of QA, or the electron flow through PS2, measured by Chl fluorescence, were detected in any of the five lichen species studied. Additionally, neither photosynthetic CO2 assimilation nor xanthophyll cycle activity or photosynthetic pigment concentration were affected by high O3 concentrations. Thus the studied lichen species have significant capacities to withstand oxidative stresses induced by high concentration of O3.

Additional key words: Anaptychia; antheraxanthin; Evernia; Hypogymnia; Parmelia; photosystem 2; quantum efficiency; violaxanthin; zeaxanthin

Prepublished online: November 1, 2000; Published: August 1, 2000  Show citation

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Calatayud, A., Temple, P.J., & Barreno, E. (2000). Chlorophyll a Fluorescence Emission, Xanthophyll Cycle Activity, and Net Photosynthetic Rate Responses to Ozone in Some Foliose and Fruticose Lichen Species. Photosynthetica, 38(2), 281-286. doi: 10.1023/A:1007214915785
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    References

    1. Aarnes, H., Baumann, C., Eriksen, A.B., Solås, A., Sundbye, A., Sølvernes, K.A.: Photosynthesis in ozone-exposed duckweed (Lemna gibba).-Physiol. Plant. 87: 256-262, 1993. Go to original source...
    2. Abadía, J., Abadía, A.: Iron and plant pigments.-In: Barton, L., Hemmig, B. (ed.): Iron Chelation in Plants and Soil Microorganisms. Pp. 327-344. Academic Press, San Diego 1993. Go to original source...
    3. Balaguer, L., Manrique, E., Ascaso, C.: Predictability of the combined effects of sulphur dioxide and nitrate on the green-algal lichen Ramalina farinacea.-Can. J. Bot. 75: 1836-1841, 1997. Go to original source...
    4. Balaguer, L., Valladares, F., Ascaso, C., Barnes, J.D., De Los Rios, A., Manrique, E., Smith, E.C.: Potential effects of rising tropospheric concentrations of CO2 and O3 on green algal lichens.-New Phytol. 132: 641-652, 1996. Go to original source...
    5. Barreno, E., Fos, S., Calatayud, A., Gimeno, C., Arhoun, M.: Report.-Univ. Valencia and ENDESA, Valencia 1999.
    6. Bates, J.W., McNee, P.J., Mcleod, A.R.: Effects of sulphur dioxide and ozone on lichen colonization of conifers in the liphook forest fumigation project.-New Phytol. 132: 653-660, 1996. Go to original source...
    7. Bilger, W., Björkman, O.: Temperature dependence of violaxanthin de-epoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L.-Planta 184: 226-234, 1991. Go to original source...
    8. Brown, D.H., Smirnoff, N.: Observations on the effect of ozone on Cladonia rangiformis.-Lichenologist 10: 91-94, 1978. Go to original source...
    9. Calatayud, A., Sanz, M.J., Calvo, E., Barreno, E., Del Valle-Tascón, S.: Chlorophyll a fluorescence and chlorophyll content in Parmelia quercina from a polluted region of northern Castellón (Spain).-Lichenologist 28: 49-65, 1996. Go to original source...
    10. Davison, A.W., Barnes, J.D.: Effects of ozone on wild plants.-New Phytol. 139: 135-151, 1998. Go to original source...
    11. De las Rivas, J., Abadía, A., Abadía, J.: A new reversed phase-HPLC method for resolving all major higher plant photosynthetic pigments.-Plant Physiol. 91: 190-192, 1989. Go to original source...
    12. Deltoro, V.I., Gimeno, C., Calatayud, A., Barreno, E.: Effects of SO2 fumigations on photosynthetic CO2 gas exchange, chlorophyll a fluorescence emission and antioxidant enzymes in the lichens Evernia prunastri and Ramalina farinacea.-Physiol. Plant. 105: 648-654, 1999. Go to original source...
    13. Demmig, B., Björkman, O.: Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of O2 evolution in leaves of higher plants.-Planta 171: 171-184, 1987. Go to original source...
    14. Demmig-Adams, B., Adams, W.W., III, Logan, B.A., Verhoeven, A.S.: Xanthophyll cycle-dependent energy dissipation and flexible photosystem II efficiency in plants acclimated to light stress.-Aust. J. Plant Physiol. 22: 249-260, 1995. Go to original source...
    15. Egger, R., Schlee, D., Türk, R.: Changes of physiological and biochemical parameters in the lichen Hypogymnia physodes (L.) Nyl. due to the action of air pollutants-a field study.-Phyton (Austria) 34: 229-242, 1994.
    16. Eskling, M., Arvidsson, P.-O., Åkerlund, H.-E.: The xanthophyll cycle, its regulation and components.-Physiol. Plant. 100: 806-816, 1997. Go to original source...
    17. Farage, P.K., Long, S.P., Lechner, E.G., Baker, N.R.: The sequence of change within the photosynthetic apparatus of wheat following short-term exposure to ozone.-Plant Physiol. 95: 529-535, 1991. Go to original source...
    18. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    19. Godde, D., Buchhold, J.: Effect of long term fumigation with ozone on the turnover of the D-1 reaction center polypeptide of photosystem II in spruce (Picea abies).-Physiol. Plant. 86: 568-574, 1992. Go to original source...
    20. Gray, G.R., Savitch, L.V., Ivanov, A.G., Huner, N.P.A.: Photosystem II excitation pressure and development of resistance to photoinhibition. II. Adjustment of photosynthetic capacity in winter wheat and winter rye.-Plant Physiol. 110: 61-71, 1996. Go to original source...
    21. Heath, R.L.: Possible mechanisms for the inhibition of photosynthesis by ozone.-Photosynth. Res. 39: 439-451, 1994. Go to original source...
    22. Lefohn, A.S.: Surface Level Ozone Exposures and Their Effects on Vegetation.-Lewis Publ., Chelsea 1992.
    23. Mohanty, N., Yamamoto, H.Y.: Mechanism of non-photochemical chlorophyll fluorescence quenching. I. The role of the de-epoxidised xanthophyll and sequestered thylakoid membrane protons as probed by dibucaine.-J. Plant Physiol. 22: 231-238, 1995. Go to original source...
    24. Moldau, H., Sōber, J., Sōber, A.: Impact of acute ozone exposure on CO2 uptake by two cultivars of Phaseolus vulgaris.-Photosynthetica 28: 131-141, 1993.
    25. Nash, T.H., III, Sigal, L.L.: Gross photosynthetic response of lichens to short term ozone fumigations.-Bryologist 82: 280-285, 1979. Go to original source...
    26. Neubauer, C., Yamamoto, H.Y.: Membrane barriers and Mehler-peroxidase reaction limit the ascorbate available for violaxanthin de-epoxidase activity in intact chloroplasts.-Photosynth. Res. 39: 137-147, 1994. Go to original source...
    27. Öquist, G., Chow, W.S.: On the relationship between the quantum yield of Photosystem II electron transport, as determined by chlorophyll fluorescence and the quantum yield of CO2-dependent O2 evolution.-Photosynth. Res. 33: 51-62, 1992. Go to original source...
    28. Osmond, C.B.: What is photoinhibition? Some insights from comparisons of shade and sun plants.-In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis from Molecular Mechanisms to the Field. Pp. 1-24. Bios Scientific Publ., Oxford 1994.
    29. Pell, E.J., Eckardt, N., Enyedi, A.J.: Timing of ozone stress and resulting status of ribulose bisphosphate carboxylase/oxygenase and associated net photosynthesis.-New Phytol. 120: 397-405, 1992. Go to original source...
    30. Rosentreter, R., Ahmadjian, V.: Effects of ozone on the lichen Cladonia arbuscula and the Trebouxia phycobiont of Cladina stellaris.-Bryologist 80: 600-605, 1977. Go to original source...
    31. Ross, L.J., Nash, T.H., III: Effect of ozone on gross photosynthesis of lichens.-Environ. exp. Bot. 23: 71-77, 1983. Go to original source...
    32. Scheidegger, C., Schroeter, B.: Effects of ozone fumigation on epiphytic macrolichens: ultrastructure, CO2 gas exchange and chlorophyll fluorescence.-Environ. Pollut. 88: 345-354, 1995. Go to original source...
    33. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    34. Sigal, L.L., Johnston, J.W.: Lichen communities on conifers in southern California mountains: an ecological survey relative to oxidant air pollution.-Ecology 64: 1343-1354, 1986. Go to original source...
    35. Soldatini, G.F., Nali, C., Guidi, L., Lorenzini, G.: Photosynthesis of Hedera canariensis var. azorica variegated leaves as affected by ozone.-Photosynthetica 35: 247-253, 1998. Go to original source...
    36. U.S.E.P.A.: Air Quality Criteria for Ozone and Related Photochemical Oxidants. Vol. II. EPA/600/P-93/004bF.-Environmental Protection Agency, Office of Research and Development, Research Triangle Park 1996.

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