Photosynthetica 2000, 38(3):333-341 | DOI: 10.1023/A:1010905100398

Influence of Water on the Primary Photosynthetic Activity of Rhodospirillum Rubrum in Reverse Micelles

A. Srivastava1, A. Darszon2, R.J. Strasser3
1 Bioenergetics Laboratory, University of Geneva, Geneva, Switzerland
2 Institute of Biotechnology, Department of Molecular Genetics and Physiology, Universidad Nacional Autónoma de México, Cuernavaca, Morelos, Mexico
3 Bioenergetics Laboratory, University of Geneva, Geneva, Switzerland

The effect of water on the primary photosynthetic activity of purple bacterium Rhodospirillum rubrum was studied in Hexadecane-Tween-Spane (HTS)- and phospholipid (PLC)-reverse micelles. Reverse micelles offer the possibility of modulating the amount of water to which enzymes and multienzymatic complexes are exposed. Fast bacteriochlorophyll (BChl) fluorescence induction kinetics and reaction centre absorption changes at 820 nm were used as an assay for the functional transfer of bacterial cells into HTS-reverse micelles and bacterial photosynthetic complexes (BPC) into PLC-reverse micelles. Both the bacterial cells and BPC showed an increase in the rate of primary photosynthetic activity by increasing the concentration of water in the reverse micelles. The bacterial cells could be kept viable for many hours in HTS-reverse micelles in presence of 6% (v/v) water. NMR studies indicated that the photosynthetic activity was affected by the availability of water in reverse micelles. The bacterial cells in HTS or BPC in PLC reverse micelles could be used to further understand the influence of water on the organisation and function of photosynthetic complexes.

Additional key words: absorbance; bacteriochlorophyll fluorescence; hexadecane-tween-spane reverse micelles; nuclear magnetic resonance; phospholipid reverse micelles

Prepublished online: April 1, 2000; Published: October 1, 2000  Show citation

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Srivastava, A., Darszon, A., & Strasser, R.J. (2000). Influence of Water on the Primary Photosynthetic Activity of Rhodospirillum Rubrum in Reverse Micelles. Photosynthetica, 38(3), 333-341. doi: 10.1023/A:1010905100398
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