Photosynthetica 2000, 38(4):497-504 | DOI: 10.1023/A:1012445020761

Identification of Photosynthetic Mutants of Arabidopsis by Automatic Screening for Altered Effective Quantum Yield of Photosystem 2

C. Varotto1, P. Pesaresi1, D. Maiwald1, J. Kurth1, F. Salamini2, D. Leister1,3
1 Zentrum zur Identifikation von Genfunktionen durch Insertionsmutagenese bei Arabidopsis thaliana (ZIGIA), Max-Planck-Institut für Züchtungsforschung, Köln, Germany
2 Abteilung für Pflanzenzüchtung und Ertragsphysiologie, Max-Planck-Institut für Züchtungsforschung, Köln, Germany
3 Abteilung für Pflanzenzüchtung und Ertragsphysiologie, Max-Planck-Institut für Züchtungsforschung, Köln, Germany

Quantification of chlorophyll (Chl) fluorescence is a versatile tool for analysing the photosynthetic performance of plants in a non-intrusive manner. A pulse-amplitude modulated fluorometer was combined with a CNC router for the automated measurement of the effective quantum yield of photosystem 2 (Φ2) of Arabidopsis thaliana plants. About 90 000 individual plants representing 7 500 lines derived from En-transposon and T-DNA mutagenised Arabidopsis populations were screened for mutants with altered Φ2. Forty-eight recessive Φ2 mutations were identified of which most exhibit also altered pigmentation and increased photosensitivity. For three Φ2 mutants the corresponding mutated genes were identified that code all for chloroplast-located proteins. Comparison of the Φ2 mutant screen with other screening methods based on the measurement of Chl fluorescence shows that the Φ2 mutants identified are different to mutants identified by high Chl fluorescence. Some Φ2 mutants, on the contrary, are common to mutants identified by screens based on non-photochemical quenching.

Additional key words: chlorophyll fluorescence; electron transport; gene tagging

Prepublished online: August 1, 2000; Published: November 1, 2000  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Varotto, C., Pesaresi, P., Maiwald, D., Kurth, J., Salamini, F., & Leister, D. (2000). Identification of Photosynthetic Mutants of Arabidopsis by Automatic Screening for Altered Effective Quantum Yield of Photosystem 2. Photosynthetica, 38(4), 497-504. doi: 10.1023/A:1012445020761
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Abdallah, F., Salamini, F., Leister, D.: A prediction of the size and evolutionary origin of the proteome of chloroplasts of Arabidopsis.-Trends Plant Sci. 5: 141-142, 2000. Go to original source...
    2. Amin, P., Sy, D.A., Pilgrim, M.L., Parry, D.H., Nussaume, L., Hoffman, N.E.: Arabidopsis mutants lacking the 43-and 54-kilodalton subunits of the chloroplast signal recognition particle have distinct phenotypes.-Plant Physiol. 121: 61-70, 1999. Go to original source...
    3. Bennoun, P., Delepelaire, P.: Isolation of photosynthesis mutants in Chlamydomonas.-In: Edelman, M., Hallick, R.B., Chua, N.-H. (ed.): Methods in Chloroplast Molecular Biology. Pp. 25-38. Elsevier Biomedical Press, Amsterdam-New York-Oxford 1982.
    4. Bennoun, P., Levine, R.P.: Detecting mutants that have impaired photosynthesis by their increased level of fluorescence.-Plant Physiol. 42: 1284-1287, 1967. Go to original source...
    5. Davis, S.J., Kurepa, J., Vierstra, R.D.: The Arabidopsis thaliana HY1 locus, required for phytochrome-chromophore biosynthesis, encodes a protein related to heme oxygenases.-Proc. nat. Acad. Sci. USA 96: 6541-6546, 1999. Go to original source...
    6. Devereux, J., Haeberli, P., Smithies, O.: A comprehensive set of sequence analysis programs for the VAX.-Nucleic Acids Res. 12: 387-395, 1984. Go to original source...
    7. Dinkins, R.D., Bandaranayake, H., Green, B.R., Griffiths, A.J.: A nuclear photosynthetic electron transport mutant of Arabidopsis thaliana with altered expression of the chloroplast petA gene.-Curr. Genet. 25: 282-288, 1994. Go to original source...
    8. Emanuelsson, O., Nielsen, H., von Heijne, G.: ChloroP, a neural network-based method for predicting chloroplast transit peptides and their cleavage sites.-Protein Sci. 8: 978-984, 1999. Go to original source...
    9. Fisk, D.G., Walker, M.B., Barkan, A.: Molecular cloning of the maize gene crp1 reveals similarity between regulators of mitochondrial and chloroplast gene expression.-EMBO J. 18: 2621-2630, 1999. Go to original source...
    10. Frey, M., Stettner, C., Gierl, A.: A general method for gene isolation in tagging approaches: Amplification of insertion mutagenised sites (AIMS).-Plant J. 13: 717-721, 1998. Go to original source...
    11. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    12. Klimyuk, V.I., Persello-Cartieaux, F., Havaux, M., Contard-David, P., Schuenemann, D., Meiherhoff, K., Gouet, P., Jones, J.D., Hoffman, N.E., Nussaume, L.: A chromodomain protein encoded by the Arabidopsis CAO gene is a plant-specific component of the chloroplast signal recognition particle pathway that is involved in LHCP targeting.-Plant Cell 11: 87-100, 1999. Go to original source...
    13. Leister, D., Varotto, C., Pesaresi, P., Niwergall, A., Salamini, F.: Large-scale evaluation of plant growth in Arabidopsis thaliana by non-invasive image analysis.-Plant Physiol. Biochem. 37: 671-678, 1999. Go to original source...
    14. Liu, Y.G., Mitsukawa, N., Oosumi, T., Whittier, R.F.: Efficient isolation and mapping of Arabidopsis thaliana T-DNA insert junctions by thermal asymmetric interlaced PCR.-Plant J. 8: 457-463, 1995. Go to original source...
    15. Mandel, M.A., Feldmann, K.A., Herrera-Estrella, L., Rocha-Sosa, M., Leon, P.: CLAI, a novel gene required for chloroplast development, is highly conserved in evolution.-Plant J. 9: 649-658, 1996. Go to original source...
    16. Martienssen, R.A., Barkan, A., Freeling, M., Taylor, W.C.: Molecular cloning of a maize gene involved in photosynthetic membrane organization that is regulated by Robertson's Mutator.-EMBO J. 8: 1633-1639, 1989. Go to original source...
    17. Mascia, P.N., Robertson, D.S.: Studies of chloroplast development in four maize mutants defective in chlorophyll biosynthesis.-Planta 143: 207-211, 1978. Go to original source...
    18. Meurer, J., Meierhoff, K., Westhoff, P.: Isolation of high-chlorophyll-fluorescence mutants of Arabidopsis thaliana and their characterisation by spectroscopy, immunoblotting and Northern hybridisation.-Planta 198: 385-396, 1996. Go to original source...
    19. Meurer, J., Plucken, H., Kowallik, K.V., Westhoff, P.: A nuclear-encoded protein of prokaryotic origin is essential for the stability of photosystem II in Arabidopsis thaliana.-EMBO J. 17: 5286-5297, 1998. Go to original source...
    20. Miles, D.: Mutants of higher plants: maize.-Methods Enzymol. 69: 3-23, 1980. Go to original source...
    21. Muramoto, T., Kohchi, T., Yokota, A., Hwang, I., Goodman, H.M.: The Arabidopsis photomorphogenic mutant hy1 is deficient in phytochrome chromophore biosynthesis as a result of a mutation in a plastid heme oxygenase.-Plant Cell 11: 335-348, 1999. Go to original source...
    22. Nedbal, L., Soukupová, J., Kaftan, D., Whitmarsh, J., Trtílek, M.: Kinetic imaging of chlorophyll fluorescence using modulated light.-Photosynth. Res. (in press), 2000. Go to original source...
    23. Niyogi, K.K., Björkman, O., Grossman, A.R.: Chlamydomonas xanthophyll cycle mutants identified by video imaging of chlorophyll fluorescence quenching.-Plant Cell 9: 1369-1380, 1997. Go to original source...
    24. Niyogi, K.K., Grossman, A.R., Björkman, O.: Arabidopsis mutants define a central role for the xanthophyll cycle in the regulation of photosynthetic energy conversion.-Plant Cell 10: 1121-1134, 1998. Go to original source...
    25. Rochaix, J.-D.: Post-transcriptional steps in the expression of chloroplast genes.-Annu. Rev. Cell Biol. 8: 1-28, 1992. Go to original source...
    26. Roy, L.M., Barkan, A.: A SecY homologue is required for the elaboration of the chloroplast thylakoid membrane and for normal chloroplast gene expression.-J. Cell Biol. 141: 385-395, 1998. Go to original source...
    27. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer.-Photosynth. Res. 10: 51-62, 1986. Go to original source...
    28. Shikanai, T., Munekage, Y., Shimizu, K., Endo, T., Hashimoto, T.: Identification and characterization of Arabidopsis mutants with reduced quenching of chlorophyll fluorescence.-Plant Cell Physiol. 40: 1134-1142, 1999. Go to original source...
    29. Simpson, D.J., Wettstein, D. von: Macromolecular physiology of plastids. XIV. Viridis mutants in barley: genetic, fluoroscopic and ultrastructural characterisation.-Carlsberg Res. Commun. 45: 238-314, 1980. Go to original source...
    30. Somerville, C.R.: Analysis of photosynthesis with mutants of higher plants and algae.-Annu. Rev. Plant Physiol. 37: 467-507, 1986. Go to original source...
    31. Sundberg, E., Slagter, J.G., Fridborg, I., Cleary, S.P., Robinson, C., Coupland, G.: ALBINO3, an Arabidopsis nuclear gene essential for chloroplast differentiation, encodes a chloroplast protein that shows homology to proteins present in bacterial membranes and yeast mitochondria.-Plant Cell 9: 717-730, 1997. Go to original source...
    32. Varotto, C., Pesaresi, P., Meurer, J., Oelmüller, R., Steiner-Lange, S., Salamini, F., Leister, D.: Disruption of the Arabidopsis photosystem I gene psaE1 affects photosynthesis and impairs growth.-Plant J. 22: 115-124, 2000. Go to original source...
    33. Voelker, R., Mendel-Hartvig, J., Barkan, A.: Transposon-disruption of a maize nuclear gene, tha1, encoding a chloroplast SecA homologue: in vivo role of cp-SecA in thylakoid protein targeting.-Genetics 145: 467-478, 1997. Go to original source...
    34. Walbot, V.: Strategies for mutagenesis and gene cloning using transposon tagging and T-DNA insertional mutagenesis.-Annu. Rev. Plant Physiol. Plant mol. Biol. 43: 49-82, 1992. Go to original source...
    35. Walker, M.B., Roy, L.M., Coleman, E., Voelker, R., Barkan, A.: The maize tha4 gene functions in sec-independent protein transport in chloroplasts and is related to hcf106, tatA, and tatB.-J. Cell Biol. 147: 267-276, 1999. Go to original source...
    36. Wettstein, D. von, Henningsen, K.W., Boynton, J.E., Kannangara, G.C., Nielsen, O.F.: The genetic control of chloroplast development in barley.-In: Boardman, N.K., Linnane, A.W., Smilie, R.M. (ed.): Autonomy and Biogenesis of Mitochondria and Chloroplasts. Pp. 205-223. North-Holland Publ. Co., Amsterdam-London 1971.
    37. Wisman, E., Hartmann, U., Sagasser, M., Baumann, E., Palme, K., Hahlbrock, K., Saedler, H., Weisshaar, B.: Knock-out mutants from an En-1 mutagenized Arabidopsis thaliana population generate phenylpropanoid biosynthesis phenotypes.-Proc. nat. Acad. Sci. USA 95: 12432-12437, 1998. Go to original source...

    Return to the content