Photosynthetica 2001, 39(1):67-73 | DOI: 10.1023/A:1012443919022

The Effect of In Vitro Culture Conditions on the Pattern of Photoinhibition during Acclimation of Gardenia Plantlets to Ex Vitro Conditions

M.D. Serret1, M.I. Trillas1, J.L. Araus1
1 Unitat de Fisiologia Vegetal, Facultat de Biologia, Universitat de Barcelona, Barcelona, Spain

We tested the effect of growing conditions during micropropagation on the fast kinetics of chlorophyll (Chl) fluorescence of Gardenia jasminoides Ellis plantlets during a 4-week acclimation to ex vitro. We studied whether photoautotrophic growing in vitro produced plantlets with less photoinhibition impairment during acclimation. Of the growing conditions stimulating photoautotrophy in vitro, only loose tube caps had a positive effect, whereas low sucrose or sucrose-free content in the medium and high PPFD showed a negative effect. Thus, plantlets cultured with 3 % (m/v) of sucrose were subsequently less photoinhibited throughout acclimation than those cultured with low sucrose (0.5 %) or sucrose-free media. Moreover, at the end of acclimation the former plantlets showed Fv/Fm and Fv/F0 ratios typical of unstressed ex vitro plants as well as a higher Chl content and ratio of Chls to carotenoids. Plantlets cultured at a photosynthetic photon fluence density (PPFD) of 50 µmol m-2 s-1 also showed a better performance at the end of acclimation than those cultured at a higher (110 µmol m-2 s-1) PPFD. Thus except in the case of loose-tube closure, gardenia plantlets cultured in vitro under conventional sucrose concentration and PPFD are the least photoinhibited during acclimation. Nevertheless, significant interactions between the in vitro growing factors were observed at the end of acclimation.

Additional key words: carotenoids; chlorophylls; CO2; fluorescence induction; Gardenia jasminoides; irradiance; micropropagation; photosynthetic pigments; saccharose

Published: March 1, 2001  Show citation

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Serret, M.D., Trillas, M.I., & Araus, J.L. (2001). The Effect of In Vitro Culture Conditions on the Pattern of Photoinhibition during Acclimation of Gardenia Plantlets to Ex Vitro Conditions. Photosynthetica, 39(1), 67-73. doi: 10.1023/A:1012443919022
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    References

    1. Araus, J.L., Hogan, K.P.: Leaf structure and patterns of photoinhibition in two neotropical palms in clearings and forest understory during the dry season.-Amer. J. Bot. 81: 726-738, 1994. Go to original source...
    2. Babani, F., Lichtenthaler, H.K.: Light-induced and age-dependent development of chloroplasts in etiolated barley leaves as visualized by determination of photosynthetic pigments, CO2 assimilation rates and different kinds of chlorophyll fluorescence ratios.-J. Plant Physiol. 148: 555-566, 1996. Go to original source...
    3. Baker, N.R., Horton, P.: Chlorophyll fluorescence quenching during photoinhibition.-In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 145-168. Elsevier Science Publ., Amsterdam-New York-Oxford 1987.
    4. Björkman, O.: High-irradiance stress in higher plants and interaction with other stress factors.-In: Biggins, J. (ed.): Progress in Photosynthesis Research. Vol. 4. Pp. 11-18. Martinus Nijhoff Publ., Dordrecht-Boston-Lancaster 1986. Go to original source...
    5. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77 K among vascular plants of diverse origins.-Planta 170: 489-504, 1987. Go to original source...
    6. Bolhàr-Nordenkampf, H.R., Long, S.P., Baker, N.R., Öquist, G., Schreiber, U., Lechner, E.G.: Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation.-Funct. Ecol. 3: 497-514, 1989. Go to original source...
    7. Capellades, M., Fontarnau, R., Carulla, C., Debergh, P.C.: Environment influences anatomy of stomata and epidermal cells in tissue-cultured Rosa multiflora.-J. amer. Soc. hort. Sci. 115: 141-145, 1990a. Go to original source...
    8. Capellades, M., Lemeur, R., Debergh, P.C.: Kinetics of chlorophyll fluorescence in micropropagated rose shootlets.-Photosynthetica 24: 190-193, 1990b.
    9. Capellades, M., Lemeur, R., Debergh, P.C.: Effects of sucrose on starch accumulation and rate of photosynthesis in Rosa cultured in vitro.-Plant Cell Tissue Organ Cult. 25: 21-26, 1991. Go to original source...
    10. Debergh, P.C., De Meester, J., De Riek, J., Gillis, S., van Huylenbroeck, J.: Ecological and physiological aspects of tissue-cultured plants.-Acta bot. neerl. 41: 417-423, 1992. Go to original source...
    11. Demmig, B., Björkman, O.: Comparison of the effect of excessive light on chlorophyll fluorescence (77 K) and photon yield of O2 evolution in leaves of higher plants.-Planta 171: 171-184, 1987. Go to original source...
    12. Desjardins, Y.: Overview of factors influencing photosynthesis of micropropagated plantlets and their effect on acclimatization.-In: Carré, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in Vitro Cultures. Pp. 145-160. CEA, Saint-Paul-lez-Durance 1995.
    13. Dubé, S.L., Vidaver, W.: Photosynthetic competence of plantlets grown in vitro. An automated system for measurement of photosynthesis in vitro.-Physiol. Plant. 84: 409-416, 1992. Go to original source...
    14. Genoud-Gourichon, C., Sallanon, H., Coudret, A.: Effects of sucrose, agar, irradiance and CO2 concentration during the rooting phase on the acclimation of Rosa hybrida plantlets to ex vitro conditions.-Photosynthetica 32: 263-270, 1996.
    15. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    16. Georgieva, K., Lichtenthaler, H.K.: Photosynthetic activity and acclimation ability of pea plants to low and high temperature treatment as studied by means of chlorophyll fluorescence.-J. Plant Physiol. 155: 416-423, 1999. Go to original source...
    17. Grout, B.W.W., Aston, M.J.: Transplanting of cauliflower plants regenerated from meristem culture. II. Carbon dioxide fixation and the development of photosynthetic ability.-Hortic. Res. 17: 65-71, 1978.
    18. Hdider, C., Desjardins, Y.: Effects of sucrose on photosynthesis and phosphoenolpyruvate carboxylase activity of in vitro cultured strawberry plantlets.-Plant Cell Tissue Org. Cult. 36: 27-33, 1994. Go to original source...
    19. Jorba, J.: [Physiological and Structural Changes in the Prunus persica × Prunus dulcis Hybrid During the Acclimation of Plantlets Cultured in Vitro.]-PhD Thesis. University of Barcelona, Barcelona 1994. [In Catalan.]
    20. Kamaluddin, M., Grace, J.: Photoinhibition and light acclimation in seedlings of Bischofia javanica, a tropical forest tree from Asia.-Ann. Bot. 69: 47-52, 1992. Go to original source...
    21. Kozai, T.: Micropropagation under photoautotrophic conditions.-In: Debergh, P.C., Zimmerman, R.H. (ed.): Micropropagation. Technology and Application. Pp. 447-469. Kluwer Academic Publ., Dordrecht-Boston-London 1991a. Go to original source...
    22. Kozai, T.: Photoautotrophic micropropagation.-In Vitro cell. develop. Biol. 27P: 47-51, 1991b. Go to original source...
    23. Kozai, T., Kubota, C., Jeong, B.R.: Environmental control for the large-scale production of plants through in vitro techniques.-Plant Cell Tissue Organ Cult. 51: 49-56, 1997. Go to original source...
    24. Kozai, T., Oki, H., Fujiwara, K.: Photosynthetic characteristics of Cymbidium plantlet in vitro.-Plant Cell Tissue Org. Cult. 22: 205-211, 1990. Go to original source...
    25. Lichtenthaler, H.K.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    26. Lichtenthaler, H.K., Burkart, S.: Photosynthesis and high light stress.-Bulg. J. Plant Physiol. 25: 3-16, 1999.
    27. Lichtenthaler, H.K., Rinderle, U.: The role of chlorophyll fluorescence in the detection of stress conditions in plants.-CRC crit. Rev. anal. Chem. 19: S29-S85, 1988. Go to original source...
    28. Murphy, K.P., Santamaria, J.M., Davies, W.J., Lumsden, P.J.: Ventilation of culture vessels. I. Increased growth in vitro and survival ex vitro of Delphinium.-J. hort. Sci. Biotechnol. 73: 725-729, 1998. Go to original source...
    29. Pearcy, R.W., Sims, D.A.: Photosynthetic acclimation to changing light environments: Scaling from the leaf to the whole plant.-In: Caldwell, M.M., Pearcy, R.W. (ed.): Exploitation of Environmental Heterogeneity by Plants. Ecophysiological Processes Above-and Belowground. Pp. 145-174. Academic Press, San Diego-New York-Boston-London-Sydney-Tokyo-Toronto 1994. Go to original source...
    30. Pospíšilová, J., Čatský, J., Šesták, Z.: Photosynthesis in plants cultivated in vitro.-In: Pessarakli, M. (ed.): Handbook of Photosynthesis. Pp. 525-540. Marcel Dekker, New York-Basel-Hong Kong 1997.
    31. Pospíšilová, J., Synková, H., Haisel, D., Čatský, J., Wilhelmová, N., Šrámek, F.: Effect of elevated CO2 concentration on acclimation of tobacco plantlets to ex vitro conditions.-J. exp. Bot. 50: 119-126, 1999. Go to original source...
    32. Powles, S.B.: Photoinhibition of photosynthesis induced by visible light.-Annu. Rev. Plant Physiol. 35: 15-44, 1984. Go to original source...
    33. Rintamäki, E., Salo, R., Aro, E.-M.: Rapid turnover of the D1 reaction-center protein of photosystem II as a protection mechanism against photoinhibition in a moss, Ceratodon purpureus (Hedw.) Brid.-Planta 193: 520-529, 1994. Go to original source...
    34. Rival, A., Beulé, T., Lavergne, D., Nato, A., Havaux, M., Puard, M.: Development of photosynthetic characteristics in oil palm during in vitro micropropagation.-J. Plant Physiol. 150: 520-527, 1997. Go to original source...
    35. Schreiber, U., Bilger, W.: Progress in chlorophyll fluorescence research: major developments during the past years in retrospect.-Prog. Bot. 54: 150-175, 1993. Go to original source...
    36. Seko, Y., Nishimura, M.: Effect of CO2 and light on survival and growth of rice regenerants grown in vitro on sugar-free medium.-Plant Cell Tissue Organ Cult. 46: 257-264, 1996. Go to original source...
    37. Serret, M.D., Trillas, M.I.: Effect of light and sucrose levels on the anatomy, ultrastructure, and photosynthesis of Gardenia jasminoides Ellis leaflets cultured in vitro.-Int. J. Plant Sci. 116: 281-289, 2000. Go to original source...
    38. Serret, M.D., Trillas, M.I., Matas, J., Araus, J.L.: Development of photoautotrophy and photoinhibition of Gardenia jasminoides plantlets during micropropagation.-Plant Cell Tissue Organ Cult. 45: 1-16, 1996. Go to original source...
    39. Serret, M.D., Trillas, M., Matas, J., Araus, J.L.: The effect of different closure types, light, and sucrose concentrations on carbon isotope composition and growth of Gardenia jasminoides plantlets during micropropagation and subsequent acclimation ex vitro.-Plant Cell Tissue Organ Cult. 47: 217-230, 1997. Go to original source...
    40. Solárová, J., Pospíšilová, J.: Effect of carbon dioxide enrichment during in vitro cultivation and acclimation to ex vitro conditions.-Biol. Plant. 39: 23-30, 1997. Go to original source...
    41. Synková, H.: Sucrose affects the photosynthetic apparatus and the acclimation of transgenic tobacco to ex vitro culture.-Photosynthetica 33: 403-412, 1997.
    42. Trillas, M.I., Serret, M.D., Jorba, J., Araus, J.L.: Leaf chlorophyll fluorescence changes during acclimatization of the rootstock GF677 (peach × almond) and propagation of Gardenia jasminoides E.-In: Carré, F., Chagvardieff, P. (ed.): Ecophysiology and Photosynthetic in Vitro Cultures. Pp. 161-168. CEA, Saint-Paul-lez-Durance 1995.
    43. Van Huylenbroeck, J.M., Debergh, P.C.: Impact of sugar concentration in vitro on photosynthesis and carbon metabolism during ex vitro acclimatization of Spathiphyllum plantlets.-Physiol. Plant. 96: 298-304, 1996. Go to original source...
    44. Voráčková, Z., Lipavská, H., Konečný, P.: The efficiency of transfer of plants cultivated in vitro to ex vitro conditions as affected by sugar supply.-Biol. Plant. 41: 507-513, 1998. Go to original source...
    45. Yordanov, I., Tsonev, T., Goltsev, V., Kruleva, L., Velikova, V.: Interactive effect of water deficit and high temperature on photosynthesis of sunflower and maize plants. 1. Changes in parameters of chlorophyll fluorescence induction kinetics and fluorescence quenching.-Photosynthetica 33: 391-402, 1997.

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