Photosynthetica 2001, 39(2):215-220 | DOI: 10.1023/A:1013779125330

Carbon Fixation by the Peculiar Marine Diatom Haslea Ostrearia

G. Tremblin, J. M. Robert1
1 Laboratoire de Biologie Marine (EA 2663-ISOMer), Universit[eacut, Nantes Cedex 03, France

During batch culture of Haslea ostrearia the highest carbon (14C) fixation rate was found in vivo in cells that did not accumulate the blue pigment marennine (green form). This fixation rate decreased concomitantly with the accumulation of marennine. In vitro, no phosphoenolpyruvate carboxylase (PEPC) activity was detected, but nearly equivalent activities of ribulose-1,5-bisphosphate carboxylase (RuBPC) and phosphoenolpyruvate carboxykinase (PEPCK) were found in the green form. However, the activity of RuBPC was lower than that of PEPCK during marennine accumulation. In vitro carboxylase activities were strongly inhibited by the addition of a marennine extract. A full description of this inhibition could not be confirmed within the cells because marennine accumulates in small cytoplasmic vesicles.

Additional key words: chlorophyll; phosphoenolpyruvate carboxykinase; phosphoenolpyruvate carboxylase; photosynthesis; ribulose-1,5-bisphosphate carboxylase

Published: June 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Tremblin, G., & Robert, J.M. (2001). Carbon Fixation by the Peculiar Marine Diatom Haslea Ostrearia. Photosynthetica, 39(2), 215-220. doi: 10.1023/A:1013779125330
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Beardall, J.: Photosynthesis and photorespiration in marine phytoplankton.-Aquat. Bot. 34: 105-130, 1989. Go to original source...
    2. Beardall, J., Mukerji, D., Glover, H.E., Morris, I.: The path of carbon in photosynthesis by marine phytoplankton.-J. Phycol. 12: 409-417, 1976. Go to original source...
    3. Descolas-Gros, C., De Billy, G.: Temperature adaptation of RuBP carboxylase kinetic properties in marine Antarctic diatoms.-J. exp. mar. Biol. Ecol. 108: 147-158, 1987. Go to original source...
    4. Descolas-Gros, C., Fontugne, M.R.: Carbon fixation in marine phytoplankton: carboxylase activities and stable carbon isotope ratios; physiological and paleoclimatological aspects.-Mar. Biol. 87: 1-6, 1985. Go to original source...
    5. Descolas-Gros, C., Oriol, L.: Variations in carboxylase activity in marine phytoplankton cultures. β-carboxylation in carbon flux studies.-Mar. Ecol. Progr. Ser. 85: 163-169, 1992. Go to original source...
    6. Ebata, T., Fujita, Y.: Changes in photosynthetic activity of the diatom Phaeodactylum tricornutum in a culture of limited volume.-Plant Cell Physiol. 12: 533-541, 1971.
    7. Glover, H.E.: Ribulosebisphosphate carboxylase/oxygenase in marine organisms.-Int. Rev. Cytol. 115: 67-138, 1989. Go to original source...
    8. Griffiths, D.J.: Factors affecting the photosynthetic capacity of laboratory cultures of the diatom Phaeodactylum tricornutum.-Mar. Biol. 21: 91-97, 1973. Go to original source...
    9. Lorenzen, C.J.: Determination of chlorophyll and phaeopigments: spectrophotometric equations.-Limnol. Oceanogr. 12: 343-346, 1967. Go to original source...
    10. MacIntyre, H.L., Geider, R.J.: Regulation of Rubisco activity and its potential effect on photosynthesis during mixing in a turbid estuary.-Mar. Ecol. Prog. Ser. 144: 247-264, 1996. Go to original source...
    11. MacIntyre, H.L., Geider, R.J., McKay, R.M.: Photosynthesis and regulation of Rubisco activity in net phytoplankton from Delaware Bay.-J. Phycol. 32: 718-732, 1996. Go to original source...
    12. MacIntyre, H.L., Sharkey, T.D., Geider, R.J.: Activation and deactivation of ribulose-1,5-biphosphate carboxylase/oxygenase (Rubisco) in three marine microalgae.-Photosynth. Res. 51: 93-106, 1997. Go to original source...
    13. Maestrini, S.Y., Robert, J.M.: La production micro-algale des claires à huîtres: particularités nutritionnelles; importance de l'azote organique dissous.-Ifremer Act. Coll. 5: 185-214, 1984.
    14. Mouget, J.L., Tremblin, G., Morant-Manceau, A., Morançais, M., Robert, J.M.: Long-term photoacclimatization of Haslea ostrearia (Bacilliariophyta): growth and pigment content, in relation with photosynthesis.-Eur. J. Phycol. 34: 109-115, 2000. Go to original source...
    15. Moreau, C., Robert, J.M., Rouillard, I.: Effect of iron and manganese on the growth of the diatom Haslea ostrearia in batch culture.-Cah. Biol. Mar. 35: 269-279, 1994.
    16. Morris, I.: Paths of carbon assimilation in marine phytoplankton.-In: Falkowski, P. (ed.): Primary Productivity in the Sea. Pp. 139-159. Plenum Press, New York 1980. Go to original source...
    17. Nassiri, Y., Robert, J.M., Rincé, Y., Ginsburger-Vogel, T.: The cytoplasmic fine structure of the diatom Haslea ostrearia (Bacillariophyceae) in relation to marennine production.-Phycologia 37: 84-91, 1998. Go to original source...
    18. Neuville, D., Daste, P.: Production de pigment bleu par la Diatomée Navicula ostrearia (Gaillon) Bory, maintenue en culture uni-algale sur un milieu synthétique carencé en azote nitrique.-Compt. rend. Acad. Sci. Paris, Sér. D 274: 2030-2033, 1972.
    19. Raven, J.A.: Inorganic carbon acquisition by marine autotrophs.-Adv. bot. Res. 27: 86-183, 1997. Go to original source...
    20. Reinfelder, J.R., Kraepiel, A.M., Morel, F.M.M.: Unicellular C4 photosynthesis in a marine diatom.-Nature 407: 996-999, 2000. Go to original source...
    21. Robert, J.-M.: Variations biométriques de l'algue Navicula ostrearia Bory (Diatomée pennée en culture).-Bull. Soc. Phycol. Fr. 23: 38-44, 1978.
    22. Robert, J.-M.: Fertilité des claires ostréicoles et verdissement: utilisation de l'azote par les diatomées dominantes.-Thèse Doct. Univ. Nantes, Nantes 1983.
    23. Robert, J.-M., Hallet, J.N.: Absorption spectrum in vivo of the blue pigment 'marennine' of the pennate diatom Navicula ostrearia Bory.-J. exp. Bot. 32: 341-345, 1981. Go to original source...
    24. Robert J.-M., Morançais, M., Pondaven, P., Pradier, E., Mouget, J.L., Tremblin, G.: Caractérisation et quantification d'un extrait de marennine, pigment produit par la diatomée Haslea ostrearia Simonsen.-Crypt. Algol. (in press), 2001.
    25. Robert, J.-M., Pagès, J., Prat, D.: Applications de la biomètrie cytologique à la définition des stades de développement du Navicula ostrearia Bory: incidences de l'évolution pigmentaire sur le verdissement des claires à huîtres.-Physiol. vég. 13: 225-241, 1975.
    26. Schubert, H., Tremblin, G., Robert J.-M., Sagert, S., Rincé, Y.: In vivo fluorescence measurement of photosynthesis of Haslea ostrearia Simonsen in relation to marennine content.-Diatom. Res. 10: 341-349, 1995. Go to original source...
    27. Tremblin, G., Robert, J.-M.: Comportement photosynthétique de Haslea ostrearia en relation avec sa pigmentation bleue.-Compt. rend. Acad. Sci. Paris, Sér. D 319: 939-944, 1996.
    28. Zimba, P.V., Sullivan, M.J., Glover, H.E.: Carbon fixation in cultured marine benthic diatoms.-J. Phycol. 26: 306-311, 1990. Go to original source...

    Return to the content