Photosynthetica 2001, 39(3):363-368 | DOI: 10.1023/A:1015174108937

Pre-Treatment of Bean Seedlings with Choline Compounds Increases the Resistance of Photosynthetic Apparatus to UV-B Radiation and Elevated Temperatures

V.D. Kreslavski1, T.I. Balakhnina1, M.S. Khristin1, N.G. Bukhov2
1 Institute of Basic Biological Problems, Russian Academy of Sciences, Pushchino, Russia
2 Institute of Plant Physiology, Russian Academy of Sciences, Moscow, Russia

Bean (Phaseolus vulgaris L. cv. Berbukskaya) seedlings were pre-treated with choline compounds, 19 mM 2-ethyltrimethylammonium chloride (Ch) or 1.6 mM 2-chloroethyltrimethylammonium chloride (CCh), during 24 h, then after 6 d the excised primary leaves were exposed to UV-B and high temperature stress. Chlorophyll (Chl) fluorescence, delayed light emission, accumulation of photosynthetic pigments, contents of thiobarbituric acid reactive substances, and activities of the active oxygen detoxifying enzymes (superoxide dismutase, ascorbate peroxidase, and glutathione reductase) were examined. Pre-treatment of plants with Ch or CCh enhanced the resistance of photosystem 2 (PS2) photochemistry to UV-B and heat injuries. The higher stress resistance can be explained by the increased activity of the detoxifying enzymes. The increased content of UV-B-absorbing pigments may also contribute to the enhanced resistance of choline-treated plants to UV-B radiation.

Additional key words: active oxygen species; 2-chloroethyltrimethylammonium chloride; 2-ethyltrimethylammonium chloride; heat stress; Phaseolus vulgaris; photosystem 2; ultraviolet radiation

Published: September 1, 2001  Show citation

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Kreslavski, V.D., Balakhnina, T.I., Khristin, M.S., & Bukhov, N.G. (2001). Pre-Treatment of Bean Seedlings with Choline Compounds Increases the Resistance of Photosynthetic Apparatus to UV-B Radiation and Elevated Temperatures. Photosynthetica, 39(3), 363-368. doi: 10.1023/A:1015174108937
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    References

    1. Allen, R.D.: Dissection of oxidative stress tolerance using transgenic plants.-Plant Physiol. 107: 1049-1054, 1995. Go to original source...
    2. Bornman, J.F.: Target sites of UV-B radiation in photosynthesis of higher plants.-J. Photochem. Photobiol. B 4: 145-158, 1989. Go to original source...
    3. Bukhov, N.G., Mohanty, P.: 20. Elevated temperature stress effects on photosystems: Characterization and evaluation of the nature of heat induced impairments.-In: Singhal, G.S., Renger, G., Sopory, S.K., Irrgang, K.-D., Govindjee (ed.): Concepts in Photobiology: Photosynthesis and Photomorphogenesis. Pp. 617-648. Kluwer Academic Publ., Boston-Dordrecht-London; Narosa Publishing House, New Delhi-Madras-Bombay-Calcutta-London 1999. Go to original source...
    4. Dhindsa, R.A., Matove, W.: Drought tolerance in two mosses correlated with enzymatic defense against lipid peroxidation.-J. exp. Bot. 32: 79-91, 1981. Go to original source...
    5. Enami, I., Kitamura, M., Tomo, T., Isokawa, Y., Ohta, H., Katoh, S.: Is the primary cause of thermal inactivation of oxygen evolution in spinach PS II membranes release of the extrinsic 33 kDa protein or of Mn?-Biochim. biophys. Acta 1186: 52-58, 1994. Go to original source...
    6. Fletcher, R.A., Hofstra, G.: Triazoles as potential plant protectants.-In: Berg, D., Plempel, M. (ed.): Sterol Biosynthesis Inhibitors. Pp. 321-331. Ellis Horwood, Weinheim-Cambridge 1988.
    7. Foyer, C.H., Halliwell, B.: The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism.-Planta 133: 21-25, 1976. Go to original source...
    8. Giannopolitis, N., Ries, S.K.: Superoxide dismutases I. Occurrence in higher plants.-Plant Physiol. 59: 309-314, 1977. Go to original source...
    9. Havaux, M., Greppin, H., Strasser, R.J.: Functioning of photosystems I and II in pea leaves exposed to heat stress in the presence or absence of light. Analysis using in-vivo fluorescence, absorbance, oxygen and photoacoustic measurements.-Planta 186: 88-98, 1991. Go to original source...
    10. Kobzar', E.F., Kreslavskiï, V.D., Muzafarov, E.N.: Red radiation and choline compounds influence growth and greening of wheat seedlings.-Photosynthetica 36: 333-340, 1999. Go to original source...
    11. Larson, R.A.: The antioxidants in higher plants.-Biochemistry 27: 969-978, 1988. Go to original source...
    12. Lichtenthaler, H.K., Wellburn, A.R.: Chlorophylls and carotenoids - pigments of photosynthetic biomembranes.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 148. Pp. 350-382. Academic Press, San Diego-New York-Berkeley-Boston-London-Sydney-Tokyo-Toronto 1987. Go to original source...
    13. Mackay, C.E., Senaratna, T., McKersie, B.D., Fletcher, R.A.: Ozone induced injury to cellular membranes in Triticum aestivum L. and protection by the Triasole S-3307.-Plant Cell Physiol. 128: 1271-1278, 1987.
    14. Mirecki, R.M., Teramura, A.H.: Effects of ultraviolet-B irradiance on soybean. V. The dependence of plant sensitivity on the photosynthetic photon flux density during and after leaf expansion.-Plant Physiol. 74: 475-480, 1984. Go to original source...
    15. Nakano, J., Asada, K.: Hydrogen peroxide is scavenged by ascorbate-specific peroxidase in spinach chloroplasts.-Plant Cell Physiol. 22: 867-880, 1981.
    16. Prussakova, L.D., Lukman, A.-K., Meshcheryakov, A.B.: [Effects of chlorocholine chloride on the tolerance of spring wheat to chloride salinization.]-Fiziol. Rast. 40: 776-780, 1993. [In Russ.]
    17. Rademacher, W.: New types of plant growth retardants: Additional perspectives for practical application in agriculture and horticulture.-In: Pharis, R.P., Rood, S.B. (ed.): Plant Growth Substances. Pp. 611-618. Springer-Verlag, Berlin-Heidelberg 1990. Go to original source...
    18. Renger, G., Völker, M., Eckert, H.J., Fromme, R., Hohm-Veit, S., Gräber, P.: On mechanism of photosystem II deterioration by UV-B irradiation.-Photochem. Photobiol. 49: 97-105, 1989. Go to original source...
    19. Runeckles, V.C., Krupa, S.V.: The impact of UV-B radiation and ozone effects on terrestrial vegetation.-Environ. Pollut. 83: 191-213, 1994. Go to original source...
    20. Strid, A.W., Chow, S., Anderson, J.M.: UV-B damage and protection at the molecular level in plants.-Photosynth. Res. 39: 475-489, 1994. Go to original source...
    21. Tevini, M.: UV-effects on plants.-In: Singhal, G.S., Renger, G., Sopory, S.K., Irrgang, K.-D., Govindjee (ed.): Concepts in Photobiology: Photosynthesis and Photomorphogenesis. Pp. 588-613. Kluwer Academic Publ., Boston-Dordrecht-London; Narosa Publishing House, New Delhi-Madras-Bombay-Calcutta-London 1999.
    22. Thompson, L.K., Blaylock, R., Sturtevant, J.M., Brudvig, G.W.: Molecular basis of the heat denaturation of photosystem II.-Biochemistry 28: 6686-6695, 1989. Go to original source...
    23. Wright, C.A., Crofts, A.R.: Delayed fluorescence and the high-energy state of chloroplasts.-Eur. J. Biochem. 19: 386-397, 1971. Go to original source...
    24. Yamane, Y., Kashino, Y., Koike, H., Satoh, K.: Effects of high temperatures on photosynthetic systems in spinach: Oxygen-evolving activities, fluorescence characteristics and the denaturation process.-Photosynth. Res. 57: 51-59, 1998. Go to original source...

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