Photosynthetica 2001, 39(3):377-382 | DOI: 10.1023/A:1015178209845

Estimation of Photorespiration Rate by Simultaneous Measurements of CO2, Gas Exchange Rate, and Chlorophyll Fluorescence Quenching in the C3 Plant Vigna Radiata (L.) Wilczek and the C4 Plant Amaranthus Mongostanus L.

Y. Yoshimura1, F. Kubota1, K. Hirao1
1 Graduate School of Bioresource and Bioenvironmental Sciences, Kyushu University, Hakozaki, Higashi-ku, Fukuoka, Japan

So far the photorespiration rate (RP) in a leaf has been determined as the difference between the net photosynthetic rates (PN) measured in 21 % O2 air (PN21%) and 3 % O2 air (PN3%). In the C3 plant Vigna radiata and the C4 plant Amaranthus mongostanus L., PN and chlorophyll fluorescence quenching in leaves were monitored simultaneously. RP of leaves in situ was estimated as termed RPE from the electron transport rates through photosystem 2 (PS2), and compared with RPO (PN3% - PN21%). In V. radiataRPO was 11.9 µmol(CO2) m-2 s-1 and the ratio of RPO to PN21% was 42.2 %, whereas the ratio of RPE to PN21% was 25.7 %. This suggests that RPO may be over-estimated for the real RP in normal air. In A. mongostanus, PN was almost not changed with a decrease in O2 concentration from 21 to 3 %, whereas the quantum yield of PS2 was evidently affected by the change in O2 concentration. This fact shows the presence of photorespiration in this C4 species, where RPE was equivalent to 3.8 % of PN21%.

Additional key words: electron transport rate; photosynthesis; photosystem 2; mungbean; quantum yield

Published: September 1, 2001  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Yoshimura, Y., Kubota, F., & Hirao, K. (2001). Estimation of Photorespiration Rate by Simultaneous Measurements of CO2, Gas Exchange Rate, and Chlorophyll Fluorescence Quenching in the C3 Plant Vigna Radiata (L.) Wilczek and the C4 Plant Amaranthus Mongostanus L. Photosynthetica, 39(3), 377-382. doi: 10.1023/A:1015178209845
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Akita, S.: [Studies on the differences in photosynthesis and photorespiration among crops. I. The differential responses of photosynthesis, photorespiration and dry matter production to oxygen concentration among species.]-Bull. nat. Inst. agr. Sci. D 31: 1-58, 1980. [In Jap.]
    2. Berry, J.A., Osmond, C.B., Lorimer, G.H.: Fixation of 18O during photorespiration. Kinetic and steady-state studies of the photorespiratory carbon oxidation cycle with intact leaves and isolated chloroplasts of C3 plants.-Plant Physiol. 62: 954-967, 1978. Go to original source...
    3. Björkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence charactericteristics at 77 K among vascular plants of diverse origins.-Planta 170: 489-504, 1987. Go to original source...
    4. deVeau, E.J., Burris, J.E.: Photorespiratory rates in wheat and maize as determined by 18O-labeling.-Plant Physiol. 90: 500-511, 1989. Go to original source...
    5. Di Marco, G., Iannelli, M.A., Loreto, F.: Relationship between photosynthesis and photorespiration in field-grown wheat leaves.-Photosynthetica 30: 45-51, 1994.
    6. Di Martino, C., Delfine, S., Alvino, A., Loreto, F.: Photorespiration rate in spinach leaves under moderate NaCl stress.-Photosynthetica 36: 233-242, 1999. Go to original source...
    7. Edwards, G.E., Huber, S.C., Ku, M.S.B., Rathnam, C.K.M., Gutierrez, M., Mayne, B.C.: Variation in photochemical activities of C4 plants in relation to CO2 fixation.-In: Burris, R.H., Black, C.C. (ed.): CO2 Metabolism and Plant Productivity. Pp. 83-112. University Park Press, Baltimore-London-Tokyo 1976.
    8. Edwards, G.E., Ku, M.S.B., Monson, R.K.: C4 photosynthesis and its regulation.-In: Barber, J., Baker, N.R. (ed.): Photosynthetic Mechanisms and the Environment. Pp. 287-327. Elsevier, Amsterdam-New York-Oxford 1985.
    9. Edwards, G.E., Walker, G.A.: C3, C4: Mechanism, and Cellular and Environmental Regulation, of Photosynthesis.-Blackwell Scientific, Oxford 1983.
    10. Farquhar, G.D., Caemmerer, S. von, Berry, J.A.: A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species.-Planta 149: 78-90, 1980. Go to original source...
    11. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    12. Hatch, M.D.: C4 photosynthesis: a unique blend of modified biochemistry, anatomy and ultrastructure.-Biochim. biophys. Acta 895: 81-106, 1987. Go to original source...
    13. Heber, U., Bligny, R., Streb, P., Douce, R.: Photorespiration is essential for the protection of the photosynthetic apparatus of C3 plants against photoinactivation under sunlight.-Bot. Acta 109: 307-315, 1996. Go to original source...
    14. Jenkins, C.L.D., Furbank, R.T., Hatch, M.D.: Mechanism of C4 photosynthesis. A model describing the inorganic carbon pool in bundle sheath cells.-Plant Physiol. 91: 1372-1381, 1989. Go to original source...
    15. Kozaki, A., Takeba, G.: Photorespiration protects C3 plants from photooxidation.-Nature 384: 557-560, 1996. Go to original source...
    16. Krall, J.P., Edwards, G.E.: Relationship between photosystem II activity and CO2 fixation in leaves.-Physiol. Plant. 86: 180-187, 1992. Go to original source...
    17. Ku, M.S.B., Gutierrez, M., Kanai, R., Edwards, G.E.: Photosynthesis in mesophyll protoplasts and bundle sheath cells of various type of C4 plants. II. Chlorophyll and Hill reaction studies.-Z. Pflanzenphysiol. 72: 320-337, 1974. Go to original source...
    18. Leegood, R.C.: The regulation of C4 photosynthesis.-Adv. bot. Res. 26: 251-316, 1997. Go to original source...
    19. Maroco, J.P., Ku, M.S.B., Edwards, G.E.: Oxygen sensitivity of C4 photosynthesis. Evidence from gas exchange and chlorophyll fluorescence analysis with different C4 sub-types.-Plant Cell Environ. 20: 1525-1533, 1997. Go to original source...
    20. Maroco, J.P., Ku, M.S.B., Edwards, G.E.: Utilization of O2 in the metabolic optimization of C4 photosynthesis.-Plant Cell Environ. 23: 115-121, 2000. Go to original source...
    21. Mayne, B.C., Dee, A.M., Edwards, G.E.: Photosynthesis in mesophyll protoplasts and bundle sheath cells of various types of C4 plants. III. Fluorescence emission spectra, delayed light emission, and P700 content.-Z. Pflanzenphysiol. 74: 275-291, 1975. Go to original source...
    22. Miyake, C., Yokota, A.: Determination of the rate of photoreduction of O2 in the water-water cycle in watermelon leaves and enhancement of the rate by limitation of photosynthesis.-Plant Cell Physiol. 41: 335-343, 2000. Go to original source...
    23. Muraoka, H., Tang, Y., Terashima, I., Koizumi, H., Washitani, I.: Contributions of diffusional limitation, photoinhibition and photorespiration to midday depression of photosynthesis in Arisaema heterophyllum in natural high light.-Plant Cell Environ. 23: 235-250, 2000. Go to original source...
    24. Pearcy, R.W., Ehleringer, J.: Comparative ecophysology of C3 and C4 plants.-Plant Cell Environ. 7: 1-13, 1984. Go to original source...
    25. Ruuska, S.A., Badger, M.R., Andrews, T.J., Caemmerer, S. von: Photosynthetic electron sinks in transgenic tobacco with reduced amounts of Rubisco: little evidence for significant Mehler reaction.-J. exp. Bot. 51: 357-368, 2000. Go to original source...
    26. Tokuda, S., Kubota, F., Hirao, K., Saitou, K.: Effects of leaf epidermis peeling on the CO2 exchange rate and chlorophyll fluorescence quenching, and estimation of photorespiration rate from electron transport in mungbean (Vigna radiata (L.) Wilczek) leaves.-J. Fac. Agr. Kyushu Univ. 43: 293-302, 1999. Go to original source...
    27. Tsuyama, M., Kobayashi, Y.: Estimation of the photosynthetic electron flow in intact leaves: effect of modulated light intensity on the fluorescence parameters Fv/Fm and ΔF/Fm'.-In: Garab, G. (ed.): Photosynthesis, Mechanisms and Effects. Vol. V. Pp. 4069-4072. Kluwer Academic Publ., Dordrecht-Boston-London 1999. Go to original source...
    28. Ueno, O.: Ultrastructural localization of photosynthetic and photorespiratory enzymes in epidermal, mesophyll, bundle sheath, and vascular bundle cells of the C4 dicot Amaranthus viridis.-J. exp. Bot. 52: 1003-1013, 2001. Go to original source...
    29. Zelitch, I.: Photosynthesis, Photorespiration, and Plant Productivity.-Academic Press, New York-London 1971.

    Return to the content