Photosynthetica 2002, 40(1):77-84 | DOI: 10.1023/A:1020150408908

A Chlorophyll Fluorescence Analysis of the Allocation of Radiant Energy Absorbed in Photosystem 2 Antennae of Cotton Leaves During Exposure to Chilling

D. Kornyeyev1, B.A. Logan2, A.S. Holaday3
1 Department of Biological Sciences, Texas Tech University, Lubbock, USA
2 Department of Biology, Bowdoin College, Brunswick, USA
3 Department of Biological Sciences, Texas Tech University, Lubbock, USA

When dark-acclimated cotton (Gossypium hirsutum L. cv. Coker 312) leaves, pre-treated with lincomycin to inhibit chloroplast protein repair processes, were exposed to 10 °C and a PPFD of 500 μmol m-2 s-1, the proportion of excitation energy entering photochemistry (P) increased, but only to 5 % of the total energy absorbed at steady state levels of P, which were reached at 40 min of irradiation. Thermal dissipation (D) of absorbed energy increased throughout the 360 min irradiation period and accounted for the greatest portion of absorbed energy at 10 °C. When D was partitioned into constitutive (DCON), regulated (DREG), and photoinhibitory (DPI) components, it was primarily composed of DREG, the readily reversible portion of D. However, the induction of D was slow at 10 °C. Sixty minutes were required for D to reach 70 % of the energy absorbed. Considerable absorption of energy in excess of that utilized in photochemistry or dissipated thermally (designated as E) occurred, especially during induction of P and D. Over the irradiation period, the time-dependent averaged E exhibited an inverse, linear relationship with the ratio of variable (Fv) to maximum (Fm) fluorescence (PS2 efficiency) and a linear relationship with DPI. We propose that time-dependent averaged E may be useful for estimating the potential for damage to PS2 under stressful environmental conditions.

Additional key words: energy dissipation; Gossypium hirsutum; low temperature; photoinhibition

Published: March 1, 2002  Show citation

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Kornyeyev, D., Logan, B.A., & Holaday, A.S. (2002). A Chlorophyll Fluorescence Analysis of the Allocation of Radiant Energy Absorbed in Photosystem 2 Antennae of Cotton Leaves During Exposure to Chilling. Photosynthetica, 40(1), 77-84. doi: 10.1023/A:1020150408908
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