Photosynthetica 2002, 40(1):103-108 | DOI: 10.1023/A:1020112403728

Response of Intact Cyanobacterial Cells and their Photosynthetic Apparatus to Cd2+ Ion Treatment

E. Tůmová1, D. Sofrová1
1 Biochemistry Department, Faculty of Science, Charles University, Prague, Czech Republic

Intact cells of Synechococcus elongatus were treated with different concentrations (0.1 and 1.0 mM = Cd0.1, Cd1.0) of CdCl2 for 24 h. Cd0.1 treatment stimulated growth of the cell culture and chlorophyll (Chl) a concentration in the culture. Cd1.0 inhibited both the above mentioned parameters. The oxygen evolving activity of intact cells (H2O → BQ) as well as of isolated thylakoid membranes, TM (H2O → DCPIP; H2O → PBQ + FeCy) decreased after 24 h of Cd1.0 cultivation to 7 %. Photosystem 1 (PS1) activity was less sensitive to the effect of Cd2+ than PS2 activity. CdCl2 concentration in cultivation media after 24 h of cultivation proved that the cyanobacterium cells take up these ions to a large extent from the cultivation medium. After 24 h of the Cd1.0 treatment only 12 % of the amount of Cd2+ originally added to the cultivation medium was found. The ratio of external-antenna pigments, phycocyanin, and allophycocyanin to Chl increased approximately twofold with growing Cd2+ concentration in the cultivation medium. This ratio was found in both TM and dodecylmaltoside extracts.

Additional key words: chlorophyll; dodecylmaltoside; heavy metals; photochemical activities; photosynthetic pigment content; photosystems 1 and 2; Synechococcus; toxic metals

Published: March 1, 2002  Show citation

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Tůmová, E., & Sofrová, D. (2002). Response of Intact Cyanobacterial Cells and their Photosynthetic Apparatus to Cd2+ Ion Treatment. Photosynthetica, 40(1), 103-108. doi: 10.1023/A:1020112403728
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