Photosynthetica 2002, 40(1):145-151 | DOI: 10.1023/A:1020143602973

Mercury Inhibits the Activity of the NADPH:Protochlorophyllide Oxidoreductase (POR)

K. Lenti1, F. Fodor2, B. Böddi3
1 Doctoral School of the Eötvös University, Budapest, Pázmány P. sétány 1/C, Hungary
2 Department of Plant Physiology, Eötvös University, Budapest, Pázmány P. sétány 1/C, Hungary
3 Department of Plant Anatomy, Eötvös University, Budapest, Pázmány P. sétány 1/C, Hungary

The effect of Hg++ was studied on the arrangement and photoactivity of NADPH:protochlorophyllide oxidoreductase (POR) in homogenates of dark-grown wheat (Triticum aestivum L.) leaves. 77 K fluorescence emission spectra of the homogenates were recorded before and after the irradiation of the homogenates and the spectra were deconvoluted into Gaussian components. The mercury treatment caused a precipitation of the membrane particles, which was followed by a remarkable decrease of the fluorescence yield. 10-3 M Hg++ decreased the ratio of the 655 nm-emitting protochlorophyllide (Pchlide) form to the 633 nm-emitting form. 10-2 M Hg++ shifted the short wavelength band to 629-630 nm and a 655 nm form was observed which was inactive on irradiation. This inhibition may be caused by serious alteration of the enzyme structure resulting in the trans-localisation of NADPH within the active site of POR.

Additional key words: chlorophyll formation; fluorescence emission spectra; heavy metal; Hg; protochlorophyllide; Triticumwheat

Published: March 1, 2002  Show citation

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Lenti, K., Fodor, F., & Böddi, B. (2002). Mercury Inhibits the Activity of the NADPH:Protochlorophyllide Oxidoreductase (POR). Photosynthetica, 40(1), 145-151. doi: 10.1023/A:1020143602973
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