Photosynthetica 2002, 40(3):383-387 | DOI: 10.1023/A:1022675008517
Effect of Sodium Thiosulfate on the Depletion of Photosynthetic Apparatus in Cyanobacterium Synechocystis sp. PCC 6803 Cells Grown in the Presence of Glucose
- 1 Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P. R. China
Fluorescence spectroscopy at 77 K showed that the application of glucose lead to the depletion of phycobilisomes (PBS) and photosystems (PS) 2 and 1, and that PS2 was more sensitive to glucose than PS1. The application of sodium thiosulfate, an effective scavenger of reactive oxygen intermediates, counteracted the effects of glucose. Sodium thiosulfate effectively protected photosynthetic apparatus, PS2, PS1, and PBS against glucose-induced depletion. Sodium thiosulfate showed strong capability to inhibit the disappearance of chlorophyll induced by glucose. At a relatively low concentration of glucose, the application of sodium thiosulfate can even be helpful for the assembly of photosynthetic apparatus. Hence the reactive oxygen species might be involved in the depletion of the photosynthetic apparatus in the cyanobacterium Synechocystis sp. PCC 6803 cells grown in the presence of glucose.
Additional key words: allophycocyanin; chlorophyll; fluorescence; oxygen evolution; photosystems 1 and 2; phycobilisome; phycocyanin
Published: September 1, 2002 Show citation
| ACS | AIP | APA | ASA | Harvard | Chicago | Chicago Notes | IEEE | ISO690 | MLA | NLM | Turabian | Vancouver |
Wang, Z., Xu, Y., Yang, Z., Hou, H., Jiang, G., & Kuang, T. (2002). Effect of Sodium Thiosulfate on the Depletion of Photosynthetic Apparatus in Cyanobacterium Synechocystis sp. PCC 6803 Cells Grown in the Presence of Glucose. Photosynthetica, 40(3), 383-387. doi: 10.1023/A:1022675008517
References
- Alfonso, M., Perewoska, I., Kirilovsky, D.: Redox control of psbA gene expression in the cyanobacterium Synechocystis PCC 6803. Involvement of the cytochrome b 6/f complex.-Plant Physiol. 122: 505-516, 2000.
Go to original source... - Allakhverdiev, S.I., Nishiyama, Y., Suzuki, I., Tasaka, Y., Murata, N.: Genetic engineering of the unsaturation of fatty acids in membrane lipids alters the tolerance of Synechocystis to salt stress.-Proc. nat. Acad. Sci. USA 96: 5862-5867, 1999. Go to original source...
- Arnon, D.I., Barber, J.: Photoreduction of NADP+ by isolated reaction centers of photosystem II: Requirement for plastocyanin.-Proc. nat. Acad. Sci. USA 87: 5930-5934, 1990. Go to original source...
- Dai, J.B., Wu, Q.Y., Wang, R.Y., Peng, W.M., Sheng, G.Y., Fu, J.M.: Molecular source of biomarkers by genetic engineering techniques.-Chin. Sci. Bull. 45: 1025-1030, 2000. Go to original source...
- Deshnium, P., Gombos, Z., Nishiyama, Y., Murata, N.: The action in vivo of glycine betaine in enhancement of tolerance of Synechococcus sp. strain PCC 7942 to low temperature.-J. Bacteriol. 179: 339-344, 1997. Go to original source...
- Gombos, Z., Kanervo, E., Tsvetkova, N., Sakamoto, T., Aro, E.-M., Murata, N.: Genetic enhancement of the ability to tolerate photoinhibition by introduction of unsaturated bonds into membrane glycerolipids.-Plant Physiol. 115: 551-559, 1997. Go to original source...
- Hagio, M., Gombos, Z., Várkonyi, Z., Masamoto, K., Sato, N., Tsuzuki, M., Wada, H.: Direct evidence for requirement of phosphatidylglycerol in photosystem II of photosynthesis.-Plant Physiol. 124: 795-804, 2000. Go to original source...
- Hayashi, H., Alia, Mustardy, L., Deshnium, P., Ida, M., Murata, N.: Transformation of Arabidopsis thaliana with the codA gene for choline oxidase; accumulation of glycinebetaine and enhanced tolerance to salt and cold stress.-Plant J. 12: 133-142, 1997. Go to original source...
- He, Q., Vermaas, W.: Chlorophyll a availability affects psbA translation and D1 precursor processing in vivo in Synechocystis sp. PCC 6803.-Proc. nat. Acad. Sci. USA 95: 5830-5835, 1998. Go to original source...
- Schneider, D., Berry, S., Rich, P., Seidler, A., Rögner, M.: A regulatory role of the PetM subunit in a cyanobacterial cytochrome b 6f complex.-J. biol. Chem. 276: 16780-16785, 2001. Go to original source...
- Sidler, W.A.: Phycobilisome and phycobiliprotein structures.-In: Bryant, D.A. (ed.): The Molecular Biology of Cyanobacteria. Pp. 139-216. Kluwer Academic Publ., Dordrecht-Boston-London 1994. Go to original source...
- Singh, M.: Turnover of D1 protein encoded by psbA gene in higher plants and cyanobacteria sustains photosynthetic efficiency to maintain plant productivity under photoinhibitory irradiance.-Photosynthetica 38: 161-169, 2000. Go to original source...
- Stewart, P.S., Wattanakaroon, W., Goodrum, L., Fortun, S.M., McLeod, B.R.: Electrolytic generation of oxygen partially explains electrical enhancement of tobramycin efficacy against Pseudomonas aeruginosa biofilm.-Antimicrob. Agents Chemoth. 43: 292-296, 1999. Go to original source...
- Tichy, M., Vermaas, W.: In vivo role of catalase-peroxidase in Synechocystis sp. strain PCC 6803.-J. Bacteriol. 181: 1875-1882, 1999. Go to original source...
- Wu, Q.Y., Vermaas, W.F.J.: Light-dependent chlorophyll a biosynthesis upon deletion in wild-type and photosystem I-less strains of the cyanobacterium Synechocystis sp. PCC 6803.-Plant mol. Biol. 29: 933-945, 1995. Go to original source...
- Wu, Q., Yu, J., Zhao, N., Vermaas, W.: Effects of chlorophyll availability on fluorescence components of photosystems in the ORF469-deletion mutant of cyanobacterium.-Tsinghua Sci. Tech. 4: 1544-1550, 1999.
- Yamamoto, Y.: Quality control of photosystem II.-Plant Cell Physiol. 42: 121-128, 2001. Go to original source...
- Yu, J., Wu, Q., Mao, H., Zhao, N., Vermaas, W.F.J.: Effects of chlorophyll availability on phycobilisomes in Synechocystis sp. PCC 6803.-IUBMB Life 48: 625-630, 1999. Go to original source...