Photosynthetica 2002, 40(3):389-395 | DOI: 10.1023/A:1022627125355

Effects of Chilling Temperature on the Activity of Enzymes of Sucrose Synthesis and the Accumulation of Saccharides in Leaves of Three Sugarcane Cultivars Differing in Cold Sensitivity

Yu-Chun Du1, Akihiro Nose1
1 Faculty of Agriculture, Saga University, Saga, Japan

The effects of short-term exposure to chilling temperature (10 °C) on sucrose synthesis in leaves of the cold-tolerant sugarcane cultivars Saccharum sinense R. cv. Yomitanzan and Saccharum sp. cv. NiF4, and the cold-sensitive cultivar S. officinarum L. cv. Badila were studied. Plants were grown at day/night temperatures of 30/25 °C, and then shifted to a constant day/night temperature of 10 °C. After 52-h exposure to the chilling temperature, sucrose content in the leaves of NiF4 and Yomitanzan showed a 2.5- to 3.5-fold increase relative to that of the control plants that had been left on day/night temperatures of 30/25 °C. No such increase was observed in Badila leaves. Similarly, starch content in the leaves of NiF4 and Yomitanzan was maintained high, but starch was depleted in Badila leaves after the 52-h exposure. During the chilling temperature, sucrose phosphate synthase (SPS; E.C.2.4.1.14) activity was relatively stable in the leaves of NiF4 and Yomitanzan, whereas in Badila leaves SPS activity significantly decreased. There was no significant change in cytosolic fructose-1,6-bisphosphatase activity for the three cultivars at the chilling temperature. This supports the hypothesis that: (1) on exposure to chilling temperature, sucrose content in sugarcane leaves is determined by the photosynthetic rate in the leaves, and is not related to SPS activity; (2) SPS activity in sugarcane leaves at chilling temperature is to be determined by sugar concentration in the leaves.

Additional key words: C4 plants; cold temperature; cytosolic fructose-1,6-bisphosphatase; photosynthesis; saccharides; sucrose phosphate synthase; sucrose metabolism; Saccharum

Published: September 1, 2002  Show citation

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Du, Y., & Nose, A. (2002). Effects of Chilling Temperature on the Activity of Enzymes of Sucrose Synthesis and the Accumulation of Saccharides in Leaves of Three Sugarcane Cultivars Differing in Cold Sensitivity. Photosynthetica, 40(3), 389-395. doi: 10.1023/A:1022627125355
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    References

    1. Berry, J., Björkman, O.: Photosynthetic response and adaptation to temperature in higher plants.-Annu. Rev. Plant Physiol. 31: 491-543, 1980. Go to original source...
    2. Du, Y.-C., Nose, A., Kondo, A., Wasano, K.: Diurnal changes in photosynthesis in sugarcane leaves. II. Enzyme activities and metabolite levels relating to sucrose and starch metabolisms.-Plant Production Sci. 3: 9-16, 2000. Go to original source...
    3. Du, Y.-C., Nose, A., Wasano, K.: Effects of chilling temperature on photosynthetic rates, photosynthetic enzyme activities and metabolite levels in leaves of three sugarcane species.-Plant Cell Environ. 22: 317-324, 1999. Go to original source...
    4. Du, Y.-C., Nose, A., Wasano, K., Uchida, Y.: Responses to water stress of enzyme activities and metabolite levels in relation to sucrose and starch synthesis, the Calvin cycle and the C4 pathway in sugarcane (Saccharum sp.) leaves.-Aust. J. Plant Physiol. 25: 253-260, 1998. Go to original source...
    5. Ebrahim, M.K.H., Vogg, G., Osman, M.N.E.H., Komor, E.: Photosynthetic performance and adaptation of sugarcane at suboptimal temperatures.-J. Plant Physiol. 153: 587-592, 1998a. Go to original source...
    6. Ebrahim, M.K.H., Zingsheim, O., El-Shourbagy, M.N., Moore, P.H., Komor, E.: Growth and sugar storage in sugarcane growth at temperatures below and above optimum.-J. Plant Physiol. 153: 593-602, 1998b. Go to original source...
    7. Furbank, R.T., Stitt, M., Foyer, C.H.: Intercellular compartmentation of sucrose synthesis in leaves of Zea mays L.-Planta 164: 172-178, 1985. Go to original source...
    8. Gerhardt, R., Stitt, M., Heldt, H.W.: Subcellular metabolite levels in spinach leaves. Regulation of sucrose synthesis during diurnal alternations in photosynthetic partitioning.-Plant Physiol. 83: 399-407, 1987. Go to original source...
    9. Guy, C.L., Huber, J.L.A., Huber, S.C.: Sucrose phosphate synthase and sucrose accumulation at low temperature.-Plant Physiol. 100: 502-508, 1992. Go to original source...
    10. Hare, P.D., Cress, W.A., van Staden, J.: Dissecting the roles of osmolyte accumulation during stress.-Plant Cell Environ. 21: 535-553, 1998. Go to original source...
    11. Hartt, C.E.: The effect of temperature upon translocation of C14 in sugarcane.-Plant Physiol. 40: 74-81, 1965. Go to original source...
    12. Holaday, A.S., Martindale, W., Alred, R., Brooks, A.L., Leegood, R.C.: Changes in activities of enzymes of carbon metabolism in leaves during exposure of plants to low temperature.-Plant Physiol. 98: 1105-1114, 1992. Go to original source...
    13. Huber, S.C., Huber, J.L.: Role and regulation of sucrosephosphate synthase in higher plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 431-444, 1996. Go to original source...
    14. Huber, S.C., Nielsen, T.H., Huber, J.L.A., Pharr, D.M.: Variation among species in light activation of sucrose-phosphate synthase.-Plant Cell Physiol. 30: 277-285, 1989. Go to original source...
    15. Hurry, V.M., Keerberg, O., Pärnik, T., Gardeström, P., Öquist, G.: Cold-hardening results in increased activity of enzymes involved in carbon metabolism in leaves of winter rye (Secale cereale L.).-Planta 195: 554-562, 1995a. Go to original source...
    16. Hurry, V.M, Malmberg, G., Gardeström, P., Öquist, G.: Effects of a short-term shift to low temperature and of long-term cold hardening on photosynthesis and ribulose-1,5-bisphosphate carboxylase/oxygenase and sucrose phosphate synthase activity in leaves of winter rye (Secale cereale L.).-Plant Physiol. 106: 983-990, 1994. Go to original source...
    17. Hurry, V.M, Strand, Å., Tobiaeson, M., Gardeström, P., Öquist, G.: Cold hardening of spring and winter wheat and rape results in differential effects on growth, carbon metabolism, and carbohydrate content.-Plant Physiol. 109: 697-706, 1995b. Go to original source...
    18. Jones, M.G.K., Outlaw, W.H., Lowry, O.H.: Enzymic assay of 10-7 to 10-14 moles of sucrose in plant tissues.-Plant Physiol. 60: 397-383, 1977. Go to original source...
    19. Kaiser, W.M., Förster, J.: Low CO2 prevents nitrate reduction in leaves.-Plant Physiol. 91: 970-974, 1989. Go to original source...
    20. Klein, D., Morcuende, R., Stitt, M., Krapp, A.: Regulation of nitrate reductase expression in leaves by nitrate and nitrogen metabolism is completely overridden when sugars fall below a critical level.-Plant Cell Environ. 23: 863-871, 2000. Go to original source...
    21. Lunn, J.E., Furbank, R.T.: Sucrose biosynthesis in C4 plants.-New Phytol. 143: 221-237, 1999. Go to original source...
    22. Quick, W.P., Chaves, M.M., Wendler, R., David, M., Rodrigues, M.L., Passaharinho, J.A., Pereira, J.S., Adcock, M.D., Leegood, R.C., Stitt, M.: The effect of water stress on photosynthetic carbon metabolism in four species grown under field conditions.-Plant Cell Environ. 15: 25-35, 1992. Go to original source...
    23. Reimholz, R., Geiger, M., Haake, V., Deiting, U., Krause, K.-P., Sonneward, U., Stitt, M.: Potato plants contain multiple forms of sucrose phosphate synthase, which differ in their tissue distributions, their levels during development, and their responses to low temperature.-Plant Cell Environ. 20: 291-305, 1997. Go to original source...
    24. Salerno, G.L., Pontis, H.G.: Raffinose synthesis in Chlorella vulgaris cultures after a cold shock.-Plant Physiol. 89: 648-651, 1989. Go to original source...
    25. Stitt, M., Heldt, H.W.: Control of photosynthetic sucrose synthesis by fructose 2,6-bisphosphate. Intercellular metabolite distribution and properties of the cytosolic fructosebisphosphatase in leaves of Zea mays L.-Planta 164: 179-188, 1985. Go to original source...
    26. Stitt, M., Quick, W.P.: Photosynthetic carbon partitioning: its regulation and possibilities for manipulation.-Physiol. Plant. 77: 633-641, 1989. Go to original source...
    27. Stitt, M., Wilke, I., Feil, R., Heldt, H.W.: Coarse control of sucrose-phosphate synthase in leaves: Alterations of the kinetic properties in response to the rate of photosynthesis and the accumulation of sucrose.-Planta 174: 217-230, 1988. Go to original source...
    28. Strand, Å., Hurry, V., Gustafsson, P., Gardeström, P.: Development of Arabidopsis thaliana leaves at low temperature releases the suppression of photosynthesis and photosynthetic gene expression despite the accumulation of soluble carbohydrates.-Plant J. 12: 605-614, 1997. Go to original source...
    29. Strand, Å., Hurry, V., Henkes, S., Huner, N., Gustafsson, P., Gardeström, P., Stitt, M.: Acclimation of Arabidopsis leaves developing at low temperatures. Increasing cytoplasmic volume accompanies increased activities of enzymes in the Calvin cycle and in the sucrose-biosynthesis pathway.-Plant Physiol. 119: 1387-1397, 1999. Go to original source...
    30. Sundar, D., Ramachandra Reddy, A.: Low night temperature-induced changes in photosynthesis and rubber accumulation in guayule (Parthenium argentatum Gray).-Photosynthetica 38: 421-427, 2000. Go to original source...
    31. Tognetti, J.A., Salerno, G.L., Crespi, M.D., Pontis, H.G.: Sucrose and fructan metabolism of different wheat cultivars at chilling temperatures.-Physiol. Plant. 78: 554-559, 1990. Go to original source...
    32. Usuda, H., Edwards, G.E.: Localization of glycerate kinase and some enzymes of sucrose synthesis in C3 and C4 plants.-Plant Physiol. 65: 1017-1022, 1980. Go to original source...
    33. Vassey, T.L., Quick, W.P., Sharkey, T.D., Stitt, M.: Water stress, carbon dioxide, and light effects on sucrose-phosphate synthase activity in Phaseolus vulgaris.-Physiol. Plant. 81: 37-44, 1991. Go to original source...
    34. Vassey, T.L., Sharkey, T.D.: Mild water stress of Phaseolus vulgaris plants leads to reduced starch synthesis and extractable sucrose phosphate synthase activity.-Plant Physiol. 89: 1066-1070, 1989. Go to original source...
    35. Winter, H., Huber, S.C.: Regulation of sucrose metabolism in higher plants: Localization and regulation of activity of key enzymes.-Crit. Rev. Biochem. mol. Biol. 35: 253-289, 2000. Go to original source...
    36. Zrenner, R., Stitt, M.: Comparison of the effect of rapidly and gradually developing water-stress on carbohydrate metabolism in spinach leaves.-Plant Cell Environ. 14: 939-946, 1991. Go to original source...

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