Photosynthetica 2003, 41(1):137-141 | DOI: 10.1023/A:1025833118206

Expression of vde Gene Integrated Into Tobacco Genome in Antisense and Overexpressed Ways

Ying Deng1, Rong-Cheng Lin1, Yu-Xiang Jing1, Qiang Wang1, Liang-Bi Li1, Bo-Lin Liu1, Ting-Yun Kuang1,*
1 Photosynthesis Research Center, Institute of Botany, Chinese Academy of Sciences, Beijing, China

Violaxanthin de-epoxidase (VDE) is localised in the thylakoid lumen of chloroplasts and catalyses de-epoxidation of violaxanthin into antheraxanthin and zeaxanthin. Tobacco vde gene was inserted into a binary vector pCAMBIA1301 with the hygromycin resistant gene for selection in antisense and overexpressed ways. Two constructs with antisense and overexpressed vde gene were introduced in tobacco (Nicotiana tabacum L.) using Agrobacterium tumefaciens strain LBA4404, PCR and Southern blot analyses demonstrated that the exogenous gene was integrated into genome of tobacco plants. VDE activity assay and HPLC analysis of pigments showed that the vde gene was expressed in the overexpressed transformants, whereas suppressed in the antisense ones. The chlorophyll fluorescence measurements proved that the contents of VDE in transgenic plants have a significant function in non-photochemical quenching.

Additional key words: Nicotiana; non-photochemical quenching; transgenic plants; violaxanthin de-epoxidase; xanthophyll cycle

Published: March 1, 2003  Show citation

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Deng, Y., Lin, R., Jing, Y., Wang, Q., Li, L., Liu, B., & Kuang, T. (2003). Expression of vde Gene Integrated Into Tobacco Genome in Antisense and Overexpressed Ways. Photosynthetica, 41(1), 137-141. doi: 10.1023/A:1025833118206
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