Photosynthetica 2004, 42(1):49-57 | DOI: 10.1023/B:PHOT.0000040569.17719.2a

Negative Effects of P-Buffering and pH on Photosynthetic Activity of Planktonic Desmid Species

E. Spijkerman1, E. Garcia-Mendoza2, H.C.P. Matthijs2, E. Van Hunnik3, P.F.M. Coesel1
1 Institute for Biodiversity and Ecosystem Dynamics, University of Amsterdam, Amsterdam, The Netherlands, e-mail
2 IBED/MB, University of Amsterdam, Nieuwe Achtergracht, WS Amsterdam, The Netherlands
3 Department of Plant Physiology, University of Amsterdam, Amsterdam, The Netherlands

The photosynthetic activities of three planktonic desmid species (Staurastrum brachiatum, Staurodesmus cuspidatus var. curvatus, and Staurastrum chaetoceras) were compared after adaptation to medium enriched with either a 20 mM Na+-phosphate (P) or HEPES buffer. Incubations up to 2 d were carried out at pH 6 or 8 under normal air or air enriched with 5 % CO2. Gross maximum photosynthetic rate (Pmax) and growth rate were decreased in both S. brachiatum and Std. cuspidatus at higher pH when using the HEPES buffer and this effect was independent of CO2 concentration, indicating that pH had an inhibitory effect on photosynthesis and growth in these species. The P-buffer at pH 8 caused a large decrease in Pmax and quantum yield for charge separation in photosystem 2 (PS2), compared to HEPES-buffered algae. This effect was very large in both S. brachiatum and Std. cuspidatus, two species characteristic of soft water lakes, but also significant in S. chaetoceras, a species dominant in eutrophic, hard water lakes. The decreased Pmax in P-buffer could not be related to a significant increase in cellular P content known to be responsible for inhibition in isolated chloroplasts. Experiments at pH 6 and 8 showed that two conditions, high pH and high Na+ concentration, both contributed to the decreased Pmax and quantum yield in the desmids. Effects of a P-buffer were less pronounced by using K+-P buffer. The use of P-buffer at pH 8 possibly resulted in high irradiance stress in all species, indicated by damage in the PS2 core complex. In the soft water species pH 8 resulted in increased non-photochemical quenching together with a high de-epoxidation state of the xanthophyll cycle pigments.

Additional key words: Desmidiaceae; high irradiance stress; inorganic carbon limitation; Na+; non-photochemical quenching; photosystem 2; pH-stress; Staurastrum; Staurodesmus

Published: March 1, 2004  Show citation

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Spijkerman, E., Garcia-Mendoza, E., Matthijs, H.C.P., Van Hunnik, E., & Coesel, P.F.M. (2004). Negative Effects of P-Buffering and pH on Photosynthetic Activity of Planktonic Desmid Species. Photosynthetica, 42(1), 49-57. doi: 10.1023/B:PHOT.0000040569.17719.2a
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    References

    1. Amoroso, G., Sültemeyer, D., Thyssen, C., Fock, H.P.: Uptake of HCO3-and CO2 in cells and chloroplasts from the microal-gae Chlamydomonas reinhardtii and Dunaliella tertiolecta.-Plant Physiol. 116: 193-201, 1998. Go to original source...
    2. Bailey, S., Walters, R.G., Jansson, S., Horton, P.: Acclimation of Arabidopsis thaliana to the light environment: the exis-tence of separate low light and high light responses.-Planta 213: 794-801, 2001. Go to original source...
    3. Bakker, E.P.: The role of alkali-cation transport in energy coupling of neutrophilic and acidophilic bacteria: an assessment of methods and concepts.-FEMS Microbiol. Rev. 75: 319-334, 1990. Go to original source...
    4. Birmingham, B.C., Colman, B.: Measurement of carbon dioxide compensation points of freshwater algae.-Plant Physiol. 64: 892-895, 1979. Go to original source...
    5. Brook, A.J.: The Biology of Desmids.-Blackwell Scientific Publications, Oxford 1981.
    6. Choo, K.S., Snoeijs, P., Pedersen, M.: Uptake of inorganic car-bon by Cladophora glomerata (Chlorophyta) from the Baltic Sea.-J. Phycol. 38: 493-502, 2002. Go to original source...
    7. Coesel, P.F.M.: Poor physiological adaptation to alkaline cul-ture conditions in Closterium acutum var. variabile, a plank-tonic desmid from eutrophic waters.-Eur. J. Phycol. 28: 53-57, 1993. Go to original source...
    8. Coesel, P.F.M.: On the ecological significance of a cellular mu-cilaginous envelope in planktic desmids.-Algol. Studies 73: 65-74, 1994. Go to original source...
    9. Coesel, P.F.M., Wardenaar, K.J.: Light-limited growth and pho-tosynthetic characteristics of two planktonic desmid species.-Freshwater Biol. 31: 221-226, 1994. Go to original source...
    10. Dubinsky, Z., Falkowski, P.G., Post, A.F., van Hes, U.M.: A system for measuring phytoplankton photosynthesis in a defi-ned light field with an oxygen electrode.-J. Plankton Res. 9: 607-612, 1987. Go to original source...
    11. Endo, T., Schreiber, U., Asada, K.: Suppression of quantum yield of photosystem II by hyperosmotic stress in Chlamy-domonas reinhardtii.-Plant Cell Physiol. 36: 1253-1258, 1995.
    12. Färber, A., Young, A.J., Ruban, A.V., Horton, P., Jahns, P.: Dynamics of xanthophyll-cycle activity in different antenna subcomplexes in the photosynthetic membranes of higher plants. The relationship between zeaxanthin conversion and nonphotochemical fluorescence quenching.-Plant Physiol. 115: 1609-1618, 1997. Go to original source...
    13. Flügge, U.-I.: Phosphate translocation in the regulation of photosynthesis.-J. exp. Bot. 46: 1317-1323, 1995. Go to original source...
    14. Föstner, B., Osmond, C.B., Boynton, J.E.: Very high light resistant mutant of Chlamydomonas reinhardtii: responses of photosystem II, nonphotochemical quenching and xanthophyll pigments to light and CO2.-Photosynth. Res. 67: 5-15, 2001.
    15. Gauthier, D.A., Turpin, D.H.: Interactions between inorganic phosphate (Pi) assimilation, photosynthesis and respiration in the Pi-limited green alga Selenastrum minutum.-Plant Cell Environ. 20: 12-24, 1997. Go to original source...
    16. Gimmler, H., Degenhardt, B.: Alkaliphilic and alkali-tolerant algae.-In: Rai, L.C., Gaur, J.P. (ed.): Algal Adaptation to Environmental Stresses. Physiological, Biochemical and Mo-lecular Mechanisms. Pp. 291-321. Springer, Varanasi 2001. Go to original source...
    17. Goldman, J.C., Graham, S.J.: Inorganic carbon limitation and chemical composition of two freshwater green microalgae.-Appl. environ. Microbiol. 41: 60-70, 1981. Go to original source...
    18. Goyal, A., Betsche, T., Tolbert, N.E.: Isolation of intact chloro-plasts from Dunaliella tertiolecta. - Plant Physiol. 88: 543-546, 1988. Go to original source...
    19. Hall, D.O., Rao, K.K.: Photosynthesis. 5th-Cambridge University Press, Cambridge 1994.
    20. Kalampanayil, B.D., Wimmers, L.E.: Identification and charac-terization of a salt-stress-induced plasma membrane H +-ATPase in tomato.-Plant Cell Environ. 24: 999-1005, 2001. Go to original source...
    21. Kaplan, A., Reinhold, L.: CO2 concentrating mechanisms in photosynthetic microorganisms.-Annu. Rev. Plant Physiol. Plant mol. Biol. 50: 539-570, 1999. Go to original source...
    22. Kaplan, A., Scherer, S., Lerner, M.: Nature of the light-induced H + efflux and Na + uptake in cyanobacteria.-Plant Physiol. 89: 1220-1225, 1989. Go to original source...
    23. Klein, U., Chen, C., Gibbs, M.: Photosynthetic properties of chloroplasts from Chlamydomonas reinhardii.-Plant Physiol. 72: 488-491, 1983. Go to original source...
    24. Kraay, G.W., Zapata, M., Veldhuis, M.J.W.: Separation of chlo-rophylls c1, c2, and c3 of marine phytoplankton by reversed-phase-C18-high-performance liquid chromatography.-J. Phycol. 28: 708-712, 1992. Go to original source...
    25. Legendre, L., Yeuh, Y.G., Crain, R., Haddock, N., Heinstein, P.F., Low, P.S.: Phospholipase C activation during elicitation of the oxidative burst in cultured plant cells.-J. biol. Chem. 268: 24559-24563, 1993. Go to original source...
    26. Miyairi, S.: Photosynthetic system of a thermophilic cyano-bacterium grown under high-CO2.-In: Garab, G. (ed.): Pho-tosynthesis: Mechanisms and Effects. Vol. V. Pp. 3439-3442. Kluwer Academic Publishers, Dordrecht-Boston-London 1998. Go to original source...
    27. Moroney, J.V., Mason, C.B.: The role of the chloroplast in in-organic carbon acquisition by Chlamydomonas reinhardtii.-Can. J. Bot. 69: 1017-1024, 1991. Go to original source...
    28. Murphy, J., Riley, J.P.: A modified single solution method for the determination of phosphate in natural waters.-Anal. chim. Acta 27: 31-36, 1962. Go to original source...
    29. Nichols, H.W.: Growth media-freshwater.-In Stein, J.R. (ed.): Handbook of Phycological Methods: Culture Methods and Growth Measurements. Pp. 7-24. Cambridge University Press, Cambridge 1973.
    30. Osmond, C.B.: Photorespiration and photoinhibition. Some im-plications for the energetics of photosynthesis.-Biochim. biophys. Acta 639: 77-98, 1981. Go to original source...
    31. Pratt, R.: Studies on Chlorella vulgaris. VIII. Influence on pho-tosynthesis of prolonged exposure to sodium bicarbonate and potassium bicarbonate.-Amer. J. Bot. 30: 626-629, 1943. Go to original source...
    32. Price, G.D., Badger, M.R.: Inhibition by proton buffers of pho-tosynthetic utilization of bicarbonate in Chara corallina.-Aust. J. Plant Physiol. 12: 257-267, 1985. Go to original source...
    33. Qiu, Q.S., Barkla, B.J., Vera-Estrella, R., Zhu, J.K., Schumaker, K.S.: Na + /H + exchange activity in the plasma membrane of Arabidopsis.-Plant Physiol. 132: 1041-1052, 2003. Go to original source...
    34. Raven, J.A., Beardall, J.: Carbon dioxide as the exogenous in-organic carbon source for Batrachospermum and Lemanea.-Brit. Phycol. J. 16: 165-175, 1981. Go to original source...
    35. Rotatore, C., Colman, B.: Uptake of inorganic carbon by isola-ted chloroplasts of the unicellular green alga Chlorella ellipsoidea.-Plant Physiol. 93: 1597-1600, 1990. Go to original source...
    36. Rotatore, C., Colman, B.: The acquisition and accumulation of inorganic carbon by the unicellular green alga Chlorella ellipsoidea.-Plant Cell Environ. 14: 377-382, 1991. Go to original source...
    37. ©iffel, P., Braunová, Z.: Release and aggregation of the light-harvesting complex in intact leaves subjected to strong CO2 deficit.-Photosynth. Res. 61: 217-226, 1999. Go to original source...
    38. Stumm, W., Morgan, J.J.: Aquatic Chemistry.-Wiley Inter-science, New York 1970.
    39. Sukenik, A., Bennett, J., Falkowski, P.: Light-saturated photo-synthesis-limitation by electron transport or carbon fixation?-Biochim. biophys. Acta 891: 205-215, 1987. Go to original source...
    40. Takahashi, K., Isobe, M., Muto, S.: Mastoparan induces an in-crease in cytosolic calcium ion concentration and subsequent activation of protein kinases in tobacco suspension culture cells.-Biochim. biophys. Acta 1401: 339-346, 1998. Go to original source...
    41. Webb, W.L., Newton, M., Starr, D.: Carbon dioxide exchange of Alnus rubra.: A mathematical model.-Oecologia 17: 281-291, 1974. Go to original source...
    42. Zonneveld, C.: Modeling effects of photoadaptation on the pho-tosynthesis-irradiance curve.-J. theor. Biol. 186: 381-388, 1997. Go to original source...

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