Photosynthetica 2004, 42(2):257-262 | DOI: 10.1023/B:PHOT.0000040598.48732.af

Factors Limiting Photosynthetic Recovery in Sweet Pepper Leaves After Short-Term Chilling Stress Under Low Irradiance

X. G. Li1,2, X. M. Wang3, Q. W. Meng4, Q. Zou4
1 College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, P. R. China
2 High-Tech Research Center, Crop Institute, Shandong Academy of Agricultural Sciences, Ji'nan, Shandong, P. R. China
3 College of Population, Resources and Environment, Shandong Normal University, Ji'nan, Shandong, 250014, P. R. China
4 College of Life Sciences, Shandong Agricultural University, Tai'an, Shandong, P. R. China, e-mail

The effects of chilling treatment (4 °C) under low irradiance, LI (100 μmol m-2 s-1) and in the dark on subsequent recovery of photosynthesis in chilling-sensitive sweet pepper leaves were investigated by comparing the ratio of quantum yields of photosystem (PS) 2 and CO2 assimilation, ΦPS2/ΦCO2, measured in normal air (21 % O2, NA) and low O2-air (2% O2, LOA), and by analyzing chlorophyll (Chl) a fluorescence parameters. Chilling treatment in the dark had little effect on Fv/Fm and ΦPS2/ΦCO2, but it caused the decrease of net photosynthetic rate (PN) under saturating irradiance after 6-h chilling treatment, indicating that short-term chilling alone did not induce PS2 photoinhibition. Furthermore, photorespiration and Mehler reaction also did not obviously change during subsequent recovery after chilling stress in the dark. During chilling treatment under LI, there were obvious changes in Fv/Fm and ΦPS2/ΦCO2, determined in NA or LOA. Fv/Fm could recover fully in 4 h at 25 °C, and ΦPS2/ΦCO2 increased at the end of the treatment, as determined in both NA and LOA. During subsequent recovery, ΦPS2/ΦCO2 in LOA decreased faster than in NA. Thus the Mehler reaction might play an important role during chilling treatment under LI, and photorespiration was an important process during the subsequent recovery. The recovery of PN under saturating irradiance determined in NA and LOA took about 50 h, implying that there were some factors besides CO2 assimilation limiting the recovery of photosynthesis. From the progress of reduced P700 and the increase of the Mehler reaction during chilling under LI we propose that active oxygen species were the factors inducing PS1 photoinhibition, which prevented the recovery of photosynthesis in optimal conditions because of the slow recovery of the oxidizable P700.

Additional key words: Capsicum; chilling; chlorophyll fluorescence; Mehler reaction; P700; photoinhibition; photorespiration; photosystem 2

Published: June 1, 2004  Show citation

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Li, X.G., Wang, X.M., Meng, Q.W., & Zou, Q. (2004). Factors Limiting Photosynthetic Recovery in Sweet Pepper Leaves After Short-Term Chilling Stress Under Low Irradiance. Photosynthetica, 42(2), 257-262. doi: 10.1023/B:PHOT.0000040598.48732.af
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    References

    1. Allen, D.J., Ort, D.R.: Impacts of chilling temperatures on pho-tosynthesis in warm-climate plants.-Trends Plant Sci. 6: 36-42, 2001. Go to original source...
    2. Aro, E.M., Virgin, I., Andersson, B.: Photoinhibition of photo-system II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113-134, 1993. Go to original source...
    3. Aroca, R., Irigoyen, J.J., Sanchez-Diaz, A.: Photosynthetic cha-racteristic and protective mechanisms against oxidative stress during chilling and subsequent recovery in two maize varie-ties differing in chilling sensitivity.-Plant Sci. 161: 719-726, 2001. Go to original source...
    4. Asada, K.: Radical production and scavenging in the chloro-plasts.-In: Baker, N.R. (ed.): Photosynthesis and the Envi-ronment. Pp. 123-150. Kluwer Academic Publishers, Dordrecht-Boston-London 1996. Go to original source...
    5. Baba, K., Itoh, S., Hastings, G., Hoshina, S.: Photoinhibition of Photosystem I electron transfer activity in isolated Photo-system I preparations with different chlorophyll contents.-Photosynth. Res. 47: 121-130, 1996. Go to original source...
    6. Baba, K., Itoh, S., Hoshina, S.: Degradation of photosystem I reaction center proteins during photoinhibition in vitro.-In: Mathis, P. (ed.): Photosynthesis: from Light to Biosphere. Vol. II. Pp. 179-182. Kluwer Academic Publishers, Dordrecht-Boston-London 1995. Go to original source...
    7. Barber, J.: Molecular basis of the vulnerability of photosystem II to damage by light.-Aust. J. Plant Physiol. 22: 201-208, 1995. Go to original source...
    8. Eamus, D.: Influence of preconditioning upon the changes in leaf conductance and leaf water potential of soybean, induced by chilling, water stress and abscisic acid.-Aust. J. Plant Physiol. 14: 331-339, 1987. Go to original source...
    9. Eichelmann, H., Laisk, A.: Cooperation of photosystems II and I in leaves as analyzed by simultaneous measurements of chlorophyll fluorescence and transmittance at 800 nm.-Plant Cell Physiol. 41: 138-147, 2000. Go to original source...
    10. Endo, T., Shikanai, T., Takabayashi, A., Asada, K., Sato, F.: The role of chloroplastic NAD(P)H dehydrogenase in photo-protection.-FEBS Lett. 457: 5-8, 1999. Go to original source...
    11. Farquhar, G.D., Sharkey, T.D.: Stomatal conductance and pho-tosynthesis.-Annu. Rev. Plant Physiol. 33: 317-345, 1982. Go to original source...
    12. Fryer, M.J., Andrews, J.R., Oxborough, K., Blowers, D.A., Baker, N.R.: Relationship between CO2 assimilation, photo-synthetic electron transport, and active O2 metabolism in leaves of maize in the field during periods of low temperature.-Plant Physiol. 116: 571-580, 1998. Go to original source...
    13. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron trans-port and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    14. Havaux, M., Davaud, A.: Photoinhibition of photosynthesis in chilled potato leaves is not correlated with loss of Photo-system-II activity. Preferential inactivation of Photosystem I.-Photosynth. Res. 40: 75-92, 1994. Go to original source...
    15. Huner, N.P.A., Öquist, G., Sarhan, F.: Energy balance and ac-climation to light and cold.-Trends Plant Sci. 3: 224-230, 1998. Go to original source...
    16. Kingston-Smith, A.H., Harbinson, J., William, J., Foyer, C.H.: Effect of chilling on carbon assimilation, enzyme activation, and photosynthetic electron transport in the absence of photo-inhibition in maize leaves.-Plant Physiol. 114: 1039-1046, 1997. Go to original source...
    17. Kozaki, A., Takeba, G.: Photorespiration protects C3 plants from photooxidation.-Nature 384: 557-560, 1996. Go to original source...
    18. Kratsch, H.A., Wise, R.R.: The ultrastructure of chilling stress.-Plant Cell Environ. 23: 337-350, 2000. Go to original source...
    19. Krause, G.H.: Photoinhibition induced by low temperatures. In: Baker, N.R., Bowyer, J.R. (ed.): Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Field. Pp. 331-348. Bios Scientific Publishers, Oxford 1994.
    20. Leegood, R.C., Edwards, G.E.: Carbon metabolism and photo-respiration: temperature dependence in relation to other envi-ronmental factors.-In: Baker, N.R. (ed.): Photosynthesis and the Environment. Pp. 191-221. Kluwer Academic Publishers, Dordrecht-Boston-London 1996. Go to original source...
    21. Li, X.-G., Meng, Q.-W., Jiang, G.-Q., Zou, Q.: The susceptibi-lity of cucumber and sweet pepper to chilling under low irra-diance is related to energy dissipation and water-water cycle.-Photosynthetica 41: 259-265, 2003. Go to original source...
    22. Liu, P., Meng, Q.-W., Zou, Q., Zhao, S.-J., Liu, Q.-Z.: Effects of cold-hardening on chilling-induced photoinhibition of pho-tosynthesis and on xanthophyll cycle pigments in sweet pep-per.-Photosynthetica 39: 467-472, 2001. Go to original source...
    23. Martin, B., Ort, D.R., Boyer, J.S.: Impairment of photosynthesis by chilling-temperatures in tomato.-Plant Physiol. 68: 329-334, 1981. Go to original source...
    24. Osmond, C.B., Grace, S.G.: Perspectives on photoinhibition and photorespiration in the field: Quintessential inefficiencies of the light and dark reaction of photosynthesis?-J. exp. Bot. 46: 1351-1362, 1995. Go to original source...
    25. Peeler, T.C., Naylor, A.W.: A comparison of the effects of chil-ling on thylakoid electron transfer in pea (Pisum sativum L.) and cucumber (Cucumis sativus L.). - Plant Physiol. 86: 147-151, 1988. Go to original source...
    26. Savitch, L.V., Massacci, A., Gray, G.R., Huner, N.P.A.: Accli-mation to low temperature or high light mitigates sensitivity to photoinhibition: roles of the Calvin cycle and the Mehler reaction.-Aust. J. Plant Physiol. 27: 253-264, 2000. Go to original source...
    27. Sonoike, K.: Photoinhibition of photosystem I: its physiological significance in the chilling sensitivity of plants.-Plant Cell Physiol. 37: 239-247, 1996. Go to original source...
    28. Sonoike, K., Terashima, I., Iwaki, M., Itoh, S.: Destruction of photosystem I iron-sulfur centers in leaves of Cucumis sativus L. by weak illumination at chilling temperatures.-FEBS Lett. 362: 235-238, 1995. Go to original source...
    29. Streb, P., Shang, W., Feierabend, J., Bligny, R.: Divergent stra-tegies of photoprotection in high-mountain plants.-Planta 207: 313-324, 1998. Go to original source...
    30. Terashima, I., Funayama, S., Sonoike, K.: The site of photo-inhibition in leaves of Cucumis sativus L. at low temperatures is photosystem I, not system II. - Planta 193: 300-306, 1994. Go to original source...
    31. Terashima, I., Noguchi, K., Itoh-Nemoto, T., Park, Y.M., Kubo, A., Tanaka, K.: The cause of PSI photoinhibition at low tem-peratures in leaves of Cucumis sativus, a chilling-sensitive plant.-Physiol. Plant. 103: 295-303, 1998. Go to original source...
    32. Tjus, S.E., Møller, B.L., Scheller, H.V.: Photosystem I is an early target of photoinhibiton in barley illuminated at chilling temperatures.-Plant Physiol. 116: 755-764, 1998. Go to original source...
    33. Wise, R.R.: Chilling-enhanced photooxidation: The production, action and study of reactive oxygen species produced during chilling in the light.-Photosynth. Res. 45: 79-97, 1995. Go to original source...
    34. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. Evidence for the role of singlet oxygen and superoxide in the breakown of pigments and endogenous antioxidants.-Plant Physiol. 83: 278-282, 1987. Go to original source...

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