Photosynthetica 2005, 43(1):47-53 | DOI: 10.1007/s11099-005-7053-5

Irradiance influences contents of photosynthetic pigments and proteins in tropical grasses and legumes

M. J. Baig1, A. Anand1, P. K. Mandal1, R. K. Bhatt1
1 Indian Grassland and Fodder Research Institute, Jhansi, India

Three tropical range grasses (Cenchrus ciliaris, Dichanthium annulatum, and Panicum antidotale) and two range legumes [Macroptilium atropurpureum (siratro) and Stylosanthes hamata (stylo)] were grown under four irradiances, i.e. 100 (I100, control), 75 (I75), 50 (I50), and 25 (I25) % of full sunlight. Accumulation of chlorophyll (Chl) b increased but that of Chl a decreased under low irradiances. The greater accumulation of Chl (a+b) in grasses (particularly in D. annulatum and P. antidotale) under shade predicted their shade adaptability. Among legumes Stylosanthes was more adaptive to the shade than Macroptilium due to its higher accumulation of Chl (a+b). Significant difference in the accumulation of carotenoids under I25 over I100 was observed in all the species, which shows the increase in quality of the fodder under limited irradiance. There was a significant decrease in soluble protein content in C. ciliaris under I75, however, no significant difference in protein content was observed under I50 and I25, which was also reflected in the SDS pattern with the reduction in content of polypeptides at I75 and following increase at I50 and I25. This was possibly due to reduction of light-induced protein at I75 and then expression of the stress-induced protein at further reduction of irradiance. Peroxidase activity in C. ciliaris increased with the decrease in irradiance and its isozyme pattern showed differences among all treatments, which indicated the role of different peroxidase isoforms at different irradiances.

Additional key words: carotenoids; Cenchrus; chlorophyll; Dichantium; Macroptilium; Panicum; polyacrylamide gel electrophoresis; Stylosanthes

Received: April 1, 2004; Accepted: October 14, 2004; Published: March 1, 2005  Show citation

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Baig, M.J., Anand, A., Mandal, P.K., & Bhatt, R.K. (2005). Irradiance influences contents of photosynthetic pigments and proteins in tropical grasses and legumes. Photosynthetica, 43(1), 47-53. doi: 10.1007/s11099-005-7053-5
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    References

    1. Anderson, J.M., Chow, W.S., Goodchild, D.J.: Thylakoid membrane organization in sun/shade acclimation. - Aust. J. Plant Physiol. 15: 11-26, 1988. Go to original source...
    2. Anderson, J.M., Goodchild, D.J., Boardman, N.K.: Composition of the photosystems and chlorophyll structure in extreme shade plants. - Biochim. biophys. Acta 325: 573-585, 1973. Go to original source...
    3. Anderson, J.M., Osmond, C.B.: Shade-sun responses: compromises between acclimation and photoinhibition. - In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 1-38. Elsevier, Amsterdam - New York - Oxford 1987.
    4. Armond, P.A., Arntzen, C.J.: Localization and characterization of photosystem II in grana and stroma lamellae. - Plant Physiol. 59: 398-404, 1977. Go to original source...
    5. Atanasova, L., Stefanov, D., Yordanov, I., Kornova, K., Kavardzikov, L.: Comparative characteristics of growth and photosynthesis of sun and shade leaves from normal and pendulum walnut (Juglans regia L.) trees. - Photosynthetica 41: 289-292, 2003. Go to original source...
    6. Baig, M.J., Bhat, R.K., Chatrath, A., Mandal, P.K.: Photosynthetic pigment and soluble protein accumulation in grass and legume under different light intensities. - Abstracts of National Symposium on Future of Physiological Research for the Improvement of Plant Resources. P. 2. Annamalai University, Tamil Nadu 1998.
    7. Bassi, R., Pineau, B., Dainese, P., Marquardt, J.: Carotenoid-binding proteins of photosystem II. - Eur. J. Biochem. 212: 297-303, 1993. Go to original source...
    8. Björkman, O.: Responses to different quantum flux densities. - In: Lange, O.L., Nobel, P.S., Osmond, C.B., Ziegler, H. (ed.): Physiological Plant Ecology I. Pp. 57-107. Springer-Verlag, Berlin - Heidelberg - New York 1981. Go to original source...
    9. Boardman, N.K.: Comparative photosynthesis of sun and shade plants. - Annu. Rev. Plant Physiol. 28: 355-377, 1977. Go to original source...
    10. Boardman, N.K., Björkman, O., Anderson, J.M., Goodchild, D.J., Thorne, S.W.: Photosynthetic adaptation of higher plants to light intensity: Relationship between chloroplast structure, composition of the photosystems and photosynthetic rates. - In: Avron, M. (ed.): Proceedings of the Third International Congress on Photosynthesis. Vol. III. Pp. 1809-1827. Elsevier, Amsterdam - Oxford - New York 1974.
    11. Bond, B.J., Farnsworth, B.T., Coulombe, R.A., Winner, W.E.: Foliage physiology and biochemistry in response to light gradients in conifers with varying shade tolerance. - Oecologia 120: 183-192, 1999. Go to original source...
    12. Burke, J.J., Steinback, K.E., Arntzen, C.J.: Analysis of light-harvesting pigment-protein complex of wild type and a chlorophyll-b-less mutant of barley. - Plant Physiol. 63: 237-243, 1979. Go to original source...
    13. Chance, B., Machly, A.C.: Assay of catalase and peroxidases. - Methods Enzymol. 2: 764, 1955. Go to original source...
    14. Davis, J.: Disc electrophoresis II. Methods and application of human serum protein. - Ann. New York Acad. Sci. 121: 404, 1964. Go to original source...
    15. Falbel, T.G., Staehelin, L.A., Adams, W.W., III: Analysis of xanthophyll cycle carotenoids and chlorophyll fluorescence in light intensity-dependent chlorophyll-deficient mutants of wheat and barley. - Photosynth. Res. 42: 191-202, 1994. Go to original source...
    16. Gracía-Plazaola, J.I., Becerril, J.M.: Photoprotection mechanisms in European beech (Fagus sylvatica L) seedlings from diverse climatic origins. - Trees 14: 339-343, 2000. Go to original source...
    17. Guillot-Salomon, T., Tuquet, C., Lubac, M de, Hallais, M.-F., Signol, M.: Analyse comparative de l'ultrastructure et de la composition lipidique des chloroplasts de plantes d'ombre et de soleil. - Cytobiologie 17: 442-452, 1978.
    18. Hansen, U., Fiedler, B., Rank, B.: Variation of pigment composition and antioxidative systems along the canopy light gradient in mixed beech/oak forest: a comparative study on deciduous tree species differing in shade tolerance. - Trees 16: 354-364, 2002. Go to original source...
    19. Hiscox, J.D., Israelstam, G.F.: A method for the extraction of chlorophyll from leaf tissue without maceration. - Can. J. Bot. 57: 1332-1334, 1979. Go to original source...
    20. Hong, C.K., Bang, S.B., Han, J.S., Hong, C.H., Bang, S.B., Han, J.S.: Effects of shading net on growth and yield of Aster scaber Thunb. and Ligularia fischeri Turcz. - RDA J. agr. Sci., Horticulture 38: 462-467, 1996.
    21. Jensen, A.: Chlorophyll and carotenoids. - In: Hellbust, J.A., Craigie, J.S. (ed.): Handbook of Phycological Methods. Physiological and Biochemical Methods. Pp. 59-70. Cambridge University Press, London 1978.
    22. Laemmli, U.K.: Cleavage of structural proteins during the assembly of the head of bacteriophage T4. - Nature 227: 680-685, 1970. Go to original source...
    23. Lechowicz, W., Maternicka, K., Faltynowicz, M., Poskuta, J.: Effect of light intensity on pigments and main acyl lipids during "natural" chloroplast development in wheat seedlings. - Photosynth. Res. 8: 133-147, 1986. Go to original source...
    24. Lee, A.I., Thornber, J.P.: Analysis of the pigment stoichiometry of pigment-protein complexes from barley (Hordeum vulgare). The xanthophyll cycle intermediates occur mainly in the light-harvesting complexes of photosystem I and photosystem II. - Plant Physiol. 107: 567-574, 1995. Go to original source...
    25. Lee, S.P., Kim, S.K., Nam, M.S., Choi, B.S., Lee, S.C.: Effects of shading and organic matter applications on growth and aromatic constituents of Codonopsis lanceolata. - Korean J. Crop Sci. 41: 496-504, 1996.
    26. Leong, T.-Y., Anderson, J.M.: Adaptation of the thylakoid membranes of pea chloroplasts to light intensities. II. Regulation of electron transport capacities, electron carriers, coupling factor (CF1) activity, and rates of photosynthesis. - Photosynth. Res. 5: 117-128, 1984. Go to original source...
    27. Lichtenthaler, H.K.: Effect of biocides on the development of the photosynthetic apparatus of radish seedlings grown under strong and weak light conditions. - Z. Naturforsch. 34c: 936-940, 1979. Go to original source...
    28. Lichtenthaler, H.K.: Adaptation of leaves and chloroplasts to high quanta fluence rates. - In: Akoyunoglou, G. (ed.): Photosynthesis. Vol. VI. Pp. 273-287. Balaban Int. Science Services, Philadelphia 1981.
    29. Lichtenthaler, H.K., Babani, F., Langsdorf, G., Buschmann, C.: Measurement of differences in red chlorophyll fluorescence and photosynthetic activity between sun and shade leaves by fluorescence imaging. - Photosynthetica 38: 521-529, 2000. Go to original source...
    30. Lichtenthaler, H.K, Buschmann, C., Döll, M., Fietz, H.-J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast ultrastructure, and leaf characteristics of high and low-light plants and of sun and shade leaves. - Photosynth. Res. 2: 115-141, 1981. Go to original source...
    31. Logan, B.A., Demmig-Adams, B., Adams, W.W., III, Grace, S.C.: Antioxidants in Cucurbita pepo L. and Vinca major L. acclimated to four growth PPFDs in the field. - J. exp. Bot. 49: 1869-1879, 1998. Go to original source...
    32. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent. - J. biol. Chem. 193: 265-275, 1951. Go to original source...
    33. Mika, V., Kohoutek, A., Beran, S.: Grass quality in shaded stands. - Rostlinná Výroba (Praha) 44: 431-436, 1998.
    34. Nandal, D.P.S., Bisla, S.S.: Growing oats under trees for more and better fodder. - Agroforestry today 7(1): 12, 1995.
    35. Palis, R.K., Bustrillos, A.R.: The effect of limited light on the carbohydrate and protein content of grain sorghum. - Philippine J. Crop Sci. 1: 161-166, 1976.
    36. Park, M.S., Seo, S., Han, Y.C., Lee, J.K.: Studies on the grassland development in the forest. VIII. Effect of shading degree on the quality, digestibility and nitrate nitrogen concentration of main grasses. - J. Korean Soc. Grassland Sci. 8: 85-91, 1998.
    37. Peter, G.F., Thornber, J.P.: Biochemical composition and organization of higher plant photosystem II light harvesting pigment-proteins. - J. biol. Chem. 236: 16745-16754, 1991. Go to original source...
    38. Seemann, J.R., Sharkey, T.D., Wang, J.L., Osmond, C.B.: Environmental effects on photosynthesis, nitrogen use efficiency and metabolite pools in leaves of sun and shade plants. - Plant Physiol. 84: 796-802, 1987. Go to original source...
    39. Singh, S.: Physiological response of different crop species to low light stress. - Indian J. Plant Physiol. 37: 147-151, 1994.
    40. Stitt, M.: Limitation of photosynthesis by carbon metabolism I. Evidence for excess electron transport capacity in leaves carrying out photosynthesis in saturating light and CO2. - Plant Physiol. 81: 1115-1122, 1986. Go to original source...
    41. Thayer, S.S., Björkman, O.: Leaf xanthophyll content and composition in sun and shade determined by HPLC. - Photosynth. Res. 23: 331-343, 1990. Go to original source...
    42. Thayer, S.S., Björkman, O.: Carotenoid distribution and deep oxidation in thylakoid pigment-protein complexes from cotton leaves and bundle-sheath cells of maize. - Photosynth. Res. 33: 213-225, 1992. Go to original source...
    43. Viji, M.M., Thangaraj, M., Jayapragasam, M.: Low irradiance stress tolerance in rice (Oryza sativa L.). - Biol. Plant. 39: 251-256, 1997. Go to original source...
    44. Vyas, S.P., Kathju, S., Garg, B.K., Lahiri, A.N.: Response of clusterbean genotypes to shade. - Indian J. Plant Physiol. 1: 234-238, 1996.

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