Photosynthetica 2005, 43(2):231-236 | DOI: 10.1007/s11099-005-0038-6

Genes differentially expressed under photoinhibition stress in flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa) and their genetic origins

G. Q. Jiang1,2, J. L. Guo1,3, Q. W. Meng2, Y. Q. Chen3, H. X. Wang1, Z. A. Hu1,*, T. Y. Kuang1
1 Key Laboratory of Photosynthesis and Molecular Physiology, Institute of Botany, Chinese Academy of Sciences, Beijing, P.R. China
2 College of Life Sciences, Shandong Agricultural University, Shandong, P.R. China
3 College of Bioengineering, Fujian Normal University, Fujian, P.R. China

Genes differentially expressed under high irradiance (HI) stress in mature flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa ssp. indica) were studied by the silver-staining mRNA differential display technique. We obtained 167 differentially displayed bands on silver-stained polyacrylamide gels and searched for their genetic origins. Five cDNA fragments, which were upregulated by HI stress, were cloned and sequenced. The clones of G25×320, A31×380, and G29×280 putatively encode a cytosolic monodehydroascorbate reductase (MDAR), a phosphatidylinositol 4-kinase (PI-4-K), and a DEAH-box RNA helicase, respectively. Most differentially expressed genes in hybrid rice were inherited from parents and many of them were related with both parents, although some were derived from one parent only. The differentially expressed cDNA fragments having no distinct genetic origins indicated the involvement of some unclear mechanisms in the inheritance processes from parents to hybrid.

Additional key words: cDNA; DDRT-PCR; hybrid rice; Southern blot

Received: June 24, 2004; Accepted: January 13, 2005; Published: June 1, 2005  Show citation

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Jiang, G.Q., Guo, J.L., Meng, Q.W., Chen, Y.Q., Wang, H.X., Hu, Z.A., & Kuang, T.Y. (2005). Genes differentially expressed under photoinhibition stress in flag leaves of super-hybrid rice Liangyoupeijiu (Oryza sativa) and their genetic origins. Photosynthetica, 43(2), 231-236. doi: 10.1007/s11099-005-0038-6
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