Photosynthetica 2005, 43(2):261-266 | DOI: 10.1007/s11099-005-0042-x

Cooperation of xanthophyll cycle with water-water cycle in the protection of photosystems 1 and 2 against inactivation during chilling stress under low irradiance

X. G. Li1, Y. P. Bi1, S. J. Zhao2,3, Q. W. Meng2,3,*, Q. Zou2,3, Q. W. He1
1 High-Tech Research Center, Shandong Academy of Agricultural Sciences, Ji'nan, P.R. China
2 College of Life Sciences, Shandong Agricultural University, Tai'an, P.R. China
3 Key Lab of Crop Biology of Shandong Province, Shandong Agricultural University, Tai'an, P.R. China

The xanthophyll cycle and the water-water cycle had different functional significance in chilling-sensitive sweet pepper upon exposure to chilling temperature (4 °C) under low irradiance (100 µmol m-2 s-1) for 6 h. During chilling stress, effects of non-photochemical quenching (NPQ) on photosystem 2 (PS2) in dithiothreitol (DTT) fed leaves remained distinguishable from that of the water-water cycle in diethyldithiocarbamate (DDTC) fed leaves. In DTT-fed leaves, NPQ decreased greatly accompanied by visible inhibition of the de-epoxidized ratio of the xanthophyll cycle, and maximum photochemical efficiency of PS2 (Fv/Fm) decreased markedly. Thus the xanthophyll cycle-dependent NPQ could protect PS2 through energy dissipation under chilling stress. However, NPQ had a slighter effect on photosystem 1 (PS1) in DTT-fed leaves than in DDTC-fed leaves, whereas effects of the water-water cycle on PS1 remained distinguishable from that of NPQ. Inhibiting superoxide dismutase (SOD) activity increased the accumulation of O2, the oxidation level of P700 (P700+) decreased markedly relative to the control and DTT-fed leaves. Both Fv/Fm and NPQ changed little in DDTC-fed leaves accompanied by little change of (A+Z)/(V+A+Z). This is the active oxygen species inducing PS1 photoinhibition in sweet pepper. The water-water cycle can be interrupted easily at chilling temperature. We propose that during chilling stress under low irradiance, the xanthophyll cycle-dependent NPQ has the main function to protect PS2, whereas the water-water cycle is not only the pathway to dissipate energy but also the dominant factor causing PS1 chilling-sensitivity in sweet pepper.

Additional key words: Capsicum; chlorophyll fluorescence; irradiance; temperature

Received: October 5, 2004; Accepted: January 27, 2005; Published: June 1, 2005  Show citation

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Li, X.G., Bi, Y.P., Zhao, S.J., Meng, Q.W., Zou, Q., & He, Q.W. (2005). Cooperation of xanthophyll cycle with water-water cycle in the protection of photosystems 1 and 2 against inactivation during chilling stress under low irradiance. Photosynthetica, 43(2), 261-266. doi: 10.1007/s11099-005-0042-x
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