Photosynthetica 2005, 43(3):451-456

The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum

N. M. Topchiy1, S. K. Sytnik1, O. O. Syvash1, O. K. Zolotareva1
1 M.G. Kholodny Institute of Botany, National Academy of Sciences of Ukraine, Kyiv, Ukraine

Pisum sativum (L.) plants were grown under "white" luminescent lamps, W [45 µ mol(quantum) m-2 s-1] or under the same irradiation supplemented with narrow spectrum red light-emitting diodes (LEDs), RE [λmax = 660 nm, Δλ = 20 nm, 40 µmol(quantum) m-2 s-1]. Significant differences in the chlorophyll (Chl) a fluorescence parameters, degree of State 1-State 2 transition, and the pigment-protein contents were found in plants grown under differing spectral composition. Addition of red LEDs to the "white light" resulted in higher effective quantum yield of photosystem 2 (PS2), i.e. F'v/F'm, linear electron transport (ϕPS2), photochemical quenching (qP), and lower non-photochemical quenching (qN as well as NPQ). The RE plants were characterised by higher degree State 1-State 2 transition, i.e. they were more effective in radiant energy utilisation. Judging from the data of "green" electrophoresis of Chl containing pigment-protein complexes of plants grown under various irradiation qualities, the percentage of Chl in photosystem 2 (PS2) reaction centre complexes in RE plants was higher and there was no difference in the total Chl bound with Chl-proteins of light-harvesting complexes (LHC2). Because the ratio between oligomeric and monomeric LHC2 forms was higher in RE plants, we suggest higher LHC2 stability in these ones.

Additional key words: chlorophyll; light-emitting diodes; light-harvesting complex; non-photochemical quenching; photosystem 2; Pisum sativum; photochemical quenching; spectral composition of radiation

Received: January 27, 2005; Accepted: March 7, 2005; Published: September 1, 2005  Show citation

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Topchiy, N.M., Sytnik, S.K., Syvash, O.O., & Zolotareva, O.K. (2005). The effect of additional red irradiation on the photosynthetic apparatus of Pisum sativum. Photosynthetica, 43(3), 451-456
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    References

    1. Allen, J.F.: Thylakoid protein phosphorylation, state 1-state 2 transitions, and photosystem stoichiometry adjustment: Redox control at multiple levels of gene expression. - Physiol. Plant. 93: 196-205, 1995. Go to original source...
    2. Anderson, J.M.: P-700 content and polypeptide profile of chlorophyll-protein complexes of spinach and barley thylakoids. - Biochim. biophys. Acta 591: 113-126, 1980. Go to original source...
    3. Anderson, J.M., Osmond, C.B.: Shade-sun responses: compromises between acclimation and photoinhibition. - In: Kyle, D.J., Osmond, C.B., Arntzen, C.J. (ed.): Photoinhibition. Pp. 1-38. Elsevier, Amsterdam - New York - Oxford 1987.
    4. Arnon, D.I.: Copper enzymes in isolated chloroplasts. Polyphenoloxidase in Beta vulgaris. - Plant Physiol. 24: 1-15, 1949. Go to original source...
    5. Astaphurova, T.P., Verchoturova, G.C., Zaycheva, T.A., Rakitin, A.V., Victorova, I.A., Aminov, P.I.: [The effect of different spectral region PAR ratios on the photosynthetic metabolism of the cucumber plants.] - Vest. bashkirsk. Univ. 2: 29-32, 2001. [In Russ.]
    6. Bassi, R., Dainese, P.: The role of light harvesting complex II and of the minor chlorophyll a/b proteins in the organization of the photosystem II antenna system. - In: Baltscheffsky, M. (ed.): Current Researches in Photosynthesis. Vol. II. Pp. 209-216. Kluwer Academic Publ., Dordercht - Boston - London 1990. Go to original source...
    7. Bilger, W., Bjorkman, O.: Role of the xanthophyll cycle in photoprotection elucidated by measurements of light-induced absorbance changes, fluorescence and photosynthesis in Hedera canariensis. - Photosynth. Res. 25: 173-185, 1990. Go to original source...
    8. Carlberg, I., Andersson, B.: Changed lateral migration of phospho-LHCII in the thylakoid membrane upon acclimation of spinach to low temperatures. - FEBS Lett. 333: 10-14, 1993. Go to original source...
    9. Chow, W.S., Melis, A., Anderson, J.M.: Adjustments of photosystem stoichiometry in chloroplasts improve the quantum efficiency of photosynthesis. - Proc. nat. Acad. Sci. USA. 87: 7502-7506, 1990. Go to original source...
    10. Franklin, K.A., Whitelam, G.C.: Light signals, phytochromes and cross-talk with other environmental cues. - J. exp. Bot. 55: 271-276, 2003. Go to original source...
    11. Garab, G., Cseh, Z., Kovacs, L., Rajagopal, S., Varkonyi, Z., Wentworth, M., Mustardy, L., Der, A., Ruban, A., Papp, E., Holzenburg, A., Horton, P.: Light-induced trimer to monomer transition in the main light-harvesting antenna complex of plants: Thermo-optic mechanism. - Biochemistry 41: 15121-15129, 2002. Go to original source...
    12. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    13. Goins, G.D., Yorio, N.C., Sanwoo, M.M., Brown, C.S.: Photomorphogenesis, photosynthesis, and seed yield of wheat plants grown under red light-emitting diodes (LEDs) with and without supplemental blue lighting. - J. exp. Bot. 48: 1407-1413, 1997. Go to original source...
    14. Hodges, M., Barber, J.: Photosynthetic adaptation of pea plants grown at different light intensities: State 1-State 2 transitions and associated chlorophyll fluorescence changes. - Planta 157: 166-173, 1983. Go to original source...
    15. Horton, P., Ruban, A.V., Walters, R.G.: Regulation of light harvesting in green plants. - Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655-684, 1996. Go to original source...
    16. Iordanov, I.T., Goltsev, V.N.: Regulation of photosynthesis at the level of phosphorylation of the light-harvesting complex and photophosphorylation. - Photosynthetica 21: 236-250, 1987.
    17. Leong T.Y., Andersson, J.M.: Adaptation of the thylakoid membranes of pea chloroplast to light intensities. I. Study on the distribution of chlorophyll-protein complexes. - Photosynth. Res. 5: 105-115, 1984. Go to original source...
    18. Lichtenthaler, H.K., Burkart, S.: Photosynthesis and high light stress. - Bulg. J. Plant Physiol. 25: 3-16, 1999.
    19. Lichtenthaler, H.K., Buschmann, C., Doll, M., Fietz, H.-J., Bach, T., Kozel, U., Meier, D., Rahmsdorf, U.: Photosynthetic activity, chloroplast ultrastructure, and leaf characteristic of high-light and low-light plants and of sun and shade leaves. - Photosynth. Res. 2: 115-141, 1981. Go to original source...
    20. Lichtenthaler, H.K., Kuhn, G., Prenzel, U.: Adaptation of chloroplast-ultrastructure and of chlorophyll-protein levels to high-light and low-light growth conditions. - Z. Naturforsch. 37c: 464-475, 1982. Go to original source...
    21. Lopez-Juez, E., Buurmeijer, W.F., Heeringa, G.H., Kendrick, R.E., Wesseliuss, J.C.: Response of light-grown wild-type and long hypocotyl mutant cucumber plants to end of day far-red light. - Photochem. Photobiol. 52: 143-149, 1990. Go to original source...
    22. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide. - J. exp. Bot. 51: 659-668, 2000. Go to original source...
    23. Peter, G.F., Thornber, J.P.: Biochemical composition and organization of higher plant photosystem II light-harvesting-proteins. - J. biol. Chem. 266: 16745-16754, 1991. Go to original source...
    24. Pfannschmidt, T., Schutze, K., Brost, M., Oelmuller, R.A.: A novel mechanism of nuclear photosynthesis gene regulation by redox signals from the chloroplast during photosystem stoichiometry adjustment. - J. biol. Chem. 276: 36125-36130, 2001. Go to original source...
    25. Protasova, N.N., Uells, D.M., Dobrovol'skiy, M.V., Tsoglin, L.N.: [Spectral characteristics of light sources and features of plants growth under artificial illumination.] - Fiziol. Rast. 37: 386-396, 1990. [In Russ.]
    26. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical chlorophyll fluorescence quenching with a new type of modulation fluorometer. - Photosynth. Res. 10: 51-62, 1986. Go to original source...
    27. Smith, H., Samson, G., Fork, D.C.: Photosynthetic acclimation to shade-probing the role of phytochromes using photomorphogenic mutants of tomato. - Plant Cell Environ. 16, 929-937, 1993. Go to original source...
    28. Stroch, M., Spunda, V., Kurasova, I.: Non-radiative dissipation of absorbed excitation energy within photosynthetic apparatus of higher plants. - Photosynthetica 42: 323-337, 2004. Go to original source...
    29. Topchiy, N.M., Syvash, O.O., Fomishina, R.M.: [The influence of different light intensity and spectral composition on pigment apparatus and functional characteristics of pea chloroplasts (Pisum sativum L.).] - Ukr. bot. Zh. 61: 93-100, 2004. [In Ukrain.]
    30. Walters, R.G., Horton, P.: Acclimation of Arabidopsis thaliana to the light environment: changes in composition of the photosynthetic apparatus. - Planta 195: 248-256, 1994. Go to original source...
    31. Walters, R.G., Horton, P.: Acclimation of Arabidopsis thaliana to the light environment: changes in photosynthetic function. - Planta 197: 306-312, 1995. Go to original source...
    32. Wentworth, M., Ruban, A., Horton, P.: Kinetic analysis of non-photochemical quenching of chlorophyll fluorescence. 2. Isolated light-harvesting complexes. - Biochemistry 40: 9902-9908, 2001. Go to original source...

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