Photosynthetica 2006, 44(1):93-101 | DOI: 10.1007/s11099-005-0163-2

Interactions between nitrogen fertilization and ozone in watermelon cultivar Reina de Corazones in open-top chambers. Effects on chlorophyll a fluorescence, lipid peroxidation, and yield

A. Calatayud1,*, F. Pomares1, E. Barreno2
1 Horticulture Department and Natural Resources Department, Valencian Institut for Agricultural Research (I.V.I.A.), Agriculture, Fisheries and Food Authority, Generalitat Valenciana, Moncada, Valencia, Spain
2 Faculty of Biological Sciences, Department of Botany, University of Valencia, Burjasot, Valencia, Spain

Watermelon (Citrillus lanatus) plants were grown for two consecutive years in open-top chambers with three different ozone concentrations (O3-free air, O3 ambient, and air with additional O3; CFA, NFA, and NFA+O3) and three nitrogen fertilizer concentrations [0, 14.0, and 29.6 g N per pot; N0, N1, and N2). There was an interaction between ozone and N fertilizer for the major parameters studied. O3 and N2 treatments led to a significant decrease in maximum efficiency of photosystem 2 (PS2) photochemistry (Fv/Fm), and induced a significant decrease in the actual quantum yield of PS2 (ΦPS2), due mainly to the increased closure of PS2 reaction centres (qP) and to an increase in the non-photochemical quenching (NPQ). On the other hand, these plants exhibited an increased susceptibility to photoinhibition, which could be associated with an increased fraction of reduced QA. An increase in lipid peroxidation indicated that damage was occurring at the membrane levels. High N concentration enhanced the detrimental effects of ozone on the fluorescence parameter induction and lipid peroxidation. All these negative alterations led to a decreased yield.

Additional key words: Citrollus lanatus; photochemical and non-photochemical quenching; photoinhibition; photosystem 2; quantum yield

Received: February 14, 2005; Accepted: June 9, 2005; Published: March 1, 2006  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
Calatayud, A., Pomares, F., & Barreno, E. (2006). Interactions between nitrogen fertilization and ozone in watermelon cultivar Reina de Corazones in open-top chambers. Effects on chlorophyll a fluorescence, lipid peroxidation, and yield. Photosynthetica, 44(1), 93-101. doi: 10.1007/s11099-005-0163-2
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Barnes, J.D., Bender, J., Lyons, T.M., Borland, A.M.: Natural and man-made selection for air pollution resistance. - J. exp. Bot. 50: 1423-1435, 1999. Go to original source...
    2. Bielenberg, D.G., Lynch, J.P., Pell, E.J.: A decline in nitrogen availability affects plan responses to ozone. - New Phytol. 151: 413-425, 2001. Go to original source...
    3. Bilger, W., Bjorkman, O.: Temperature dependence of violaxanthin deepoxidation and non-photochemical fluorescence quenching in intact leaves of Gossypium hirsutum L. and Malva parviflora L. - Planta 184: 226-234, 1991. Go to original source...
    4. Brewer, R.F., Guillemet, F.B., Creveling, R.K.: Influence of N-P-K fertilization on incidence and severity of oxidant injury to mangels and spinach. - Soil Sci. 92: 298-301, 1961. Go to original source...
    5. Calatayud, A., Alvarado, J.W., Barreno, E.: Differences in ozone sensitivity in three varieties of cabbage (Brassica oleracea L.) in rural Mediterranean area. - J. Plant Physiol. 159: 863-868, 2002a. Go to original source...
    6. Calatayud, A., Deltoro, V.I., Barreno, E., del Valle-Tascon, S.: Changes in in vivo chlorophyll fluorescence in lichen thalli as a function of water content and suggestion of zeaxanthin-associated photoprotection. - Physiol. Plant. 101: 93-102, 1997. Go to original source...
    7. Calatayud, A., Ramirez, J.W., Iglesias, D.J., Barreno, E.: Effects of ozone on photosynthetic CO2 exchange, chlorophyll a fluorescence and antioxidant systems in lettuce leaves. - Plant Physiol. 116: 308-316, 2002b. Go to original source...
    8. Cao, J., Govindjee: Chlorophyll a fluorescence transient as an indicator of active and inactive photosystem II in thylakoid membranes. - Biochim. biophys. Acta 1015: 180-188, 1990. Go to original source...
    9. Cardoso-Vilhena, J., Barnes, J.: Does nitrogen supply affect the response of wheat (Triticum aestivum cv. Hanno) to the combination of elevated CO2 and O3? - J. exp. Bot. 52: 1901-1911, 2001. Go to original source...
    10. Demmig-Adams, B.: Carotenoids and photoprotection in plants: A role for the xanthophyll zeaxanthin. - Biochim. biophys. Acta 1020: 1-24, 1990. Go to original source...
    11. Demmig-Adams, B., Adams, W.W., III, Barker, D.H., Logan, B.A., Bowling, D.R., Verhoeven, A.S.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. - Physiol. Plant. 98: 253-264, 1996. Go to original source...
    12. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase. - J. exp. Bot. 32: 93-101, 1981. Go to original source...
    13. Fernandez-Bayon, J.M., Barnes, J.D., Olleranshaw, J.H, Davison, A.W.: Physiological effects of ozone on cultivars of watermelon (Citrillus lanatus) and muskmelon (Cucumis melo) widely grown in Spain. - Environ. Pollut. 81: 199-206, 1993. Go to original source...
    14. Fuhrer, J.: Agroecosystem responses to combinations of elevated CO2, ozone, and global climate exchange. - Agr. Ecosyst. Environ. 97: 1-20, 2003. Go to original source...
    15. Fuhrer, J., Skarby, L., Ashmore, M.: Critical levels for ozone effects on vegetation in Europe. - Environ. Pollut. 97: 91-106, 1997. Go to original source...
    16. Genty, B. Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    17. Genty, B., Harbinson, J., Briantais, J.-M., Baker, N.R.: The relationship between non-photochemical quenching of chlorophyll fluorescence and the rate of photosystem 2 photochemistry in leaves. - Photosynth. Res. 25: 249-257, 1990. Go to original source...
    18. Gimeno, B.S., Bermejo, V., Reinert, R.A., Zheng, Y., Barnes, J.D.: Adverse effects of ambient ozone on watermelon yield and physiology at a rural site in Eastern Spain. - New Phytol. 144: 245-260, 1999. Go to original source...
    19. Guidi, L., Nali, C., Ciompi, S., Lorenzini, G., Soldatini, G.F.: The use of chlorophyll fluorescence and leaf gas exchange as methods for studying the different responses to ozone of two bean cultivars. - J. exp. Bot. 48: 173-179, 1997. Go to original source...
    20. Guidi, L., Nali, C., Lorenzini, G., Filippi, F., Soldatini, G.F.: Effect of chronic fumigation on the photosynthetic process of poplar showing different sensitivity. - Environ. Pollut. 113: 245-254, 2001. Go to original source...
    21. Heagle, A.S., Body, D.E., Heck, W.W.: An open-top chamber to assess the impact of air pollution on plants. - J. environ. Quality 2: 365-368, 1973. Go to original source...
    22. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation. - Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
    23. Krause, G.H.: Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms. - Physiol. Plant. 74: 566-574, 1988. Go to original source...
    24. Krause, G.H., Weis, E.: Chlorophyll fluorescence and photosynthesis: The basics. - Annu. Rev. Plant Physiol. Plant mol. Biol. 42: 313-349, 1991. Go to original source...
    25. Krupa, S.V., Grunhage, L., Jager, H.J., Nosal, M., Manning, W.J., Legge, A.H., Hanewald, K.: Ambient ozone and adverse crop response: a unified view of cause and effect. - Environ. Pollut. 87: 119-126, 1995. Go to original source...
    26. Lu, C., Zhang, J.: Photosynthetic CO2 assimilation, chlorophyll fluorescence and photoinhibition as affected by nitrogen deficiency in maize plants. - Plant Sci. 151: 135-143, 2000. Go to original source...
    27. Maurer, S., Matyssek, R., Gunthardt-Goerg, M.S., Landolt, W., Einig, W.: Nutrition and the ozone sensitivity of birch (Betula pendula) 1. Responses at the leaf level. - Trees 12: 1-10, 1997. Go to original source...
    28. Owens, T.G.: In vivo chlorophyll fluorescence as a probe of photosynthetic physiology. - In: Alscher, R.G., Wellburn, A.R. (ed.): Plant Responses to the Gaseous Environment. Pp. 195-217. Chapman and Hall, London 1994. Go to original source...
    29. Pell, E.J., Winner, W.E., Vinten-Johansen, C., Mooney, H.A.: Response of radish to multiple stresses. I. Physiological and growth responses to changes in ozone and nitrogen. - New Phytol. 115: 439-446, 1990. Go to original source...
    30. Pier, J.W., Doerge, T.A.: Concurrent evaluation of agronomic, economic, and environmental aspects of trickle-irrigated watermelon production. - J. environ. Qual. 24: 79-86, 1995. Go to original source...
    31. Pomares, F., Baixauli, C., Aguilar, J.M., Tarazona, F.: [Fertilization of sands.] - In: Maroto, J.V., Miguel, A. (ed.): El Cultivo de la Sandia. Pp. 139-166. Fundacion Caja Rural Valencia, Mundi Prensa 2002. [In Span.]
    32. Ranieri, A., D'Urso, G., Nali, C., Lorenzini, G., Soldatini, G.F.: Ozone stimulates apoplastic antioxidant systems in pumpkin leaves. - Physiol. Plant. 97: 381-387, 1996. Go to original source...
    33. Reinert, R., Gimeno, B.S., Salleras, J.M., Bermejo, V., Ochoa, M.J., Tarruel, A.: Ozone effects on watermelon plants at Ebro Delta: symptomatology. - Agr. Ecosyst. Environ. 38: 41-49, 1992. Go to original source...
    34. Schreiber, U., Schliwa, U., Bilger, W.: Continuous recording of photochemical and non-photochemical fluorescence quenching with a new type of modulation fluorometer. - Photosynth. Res. 10: 51-62, 1986. Go to original source...
    35. Seaton, G.G.R., Walker, D.A.: Chlorophyll fluorescence as a measure of photosynthetic carbon assimilation. - Proc. roy. Soc. London B 242: 29-35, 1990. Go to original source...
    36. Shangguan, Z.P., Shao, M.G., Dyckmans, J.: Effects of nitrogen nutrition and water deficit on net photosynthetic rate and chlorophyll fluorescence in winter wheat. - J. Plant Physiol. 156: 46-51, 2000. Go to original source...
    37. Tjoelker, M.G., Luxmoore, R.J.: Soil nitrogen and chronic ozone stress influence physiology, growth and nutrient status of Pinus taeda L. Liriodendron tulipifera L. seedlings. - New Phytol. 119: 69-81, 1991. Go to original source...
    38. Utriainen, J., Holopainen, T.: Nitrogen availability modifies the ozone responses of Scots pine seedlings exposed in an open-field system. - Tree Physiol. 21: 1205-1213, 2001. Go to original source...

    Return to the content