Photosynthetica 2006, 44(2):221-226 | DOI: 10.1007/s11099-006-0010-0

Changes in contents of photosynthetic pigments and ribulose-1,5-bisphosphate carboxylase activity during the development of globular somatic embryo into the plantlet of Siberian ginseng

D. M. Pandey1, J. W. Kim2, W. S. Lee2, U. D. Yeo1,*
1 Faculty of Biological Science, Chonbuk National University, Jeonju, Republic of Korea
2 Department of Bioscience and Biotechnology, Microplants Co. Ltd., Jeonju, Republic of Korea

During the development of the globular embryos via heart-shaped, torpedo-shaped, and cotyledonary embryos into plantlets, contents of chlorophyll (Chl) a and b and carotenoids, and activity of ribulose-1,5-bisphosphate carboxylase (RuBPC, EC 4.1.1.39) were investigated. In the solid cultivation (SC) the contents of Chl a, Chl b, Chl a/b, and total pigments increased up to plantlet stage. In the liquid cultivation (LC), contents of Chl a, Chl b, and total pigments increased till the torpedo-shaped stage, but decreased with the further development up to plantlets stage. During SC, RuBPC activity increased up to the torpedo-shaped embryo stage, but in the LC RuPBC activity increased continuously with the progress in the developmental stages. The correlations between Chl a and RuBPC activity on the SC and LC were negative, r = -0.26 and -0.56, respectively.

Additional key words: carotenoids; chlorophyll; Eleutherococcus senticosus

Received: June 20, 2005; Accepted: December 8, 2005; Published: June 1, 2006  Show citation

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Pandey, D.M., Kim, J.W., Lee, W.S., & Yeo, U.D. (2006). Changes in contents of photosynthetic pigments and ribulose-1,5-bisphosphate carboxylase activity during the development of globular somatic embryo into the plantlet of Siberian ginseng. Photosynthetica, 44(2), 221-226. doi: 10.1007/s11099-006-0010-0
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