Photosynthetica 2006, 44(3):419-424 | DOI: 10.1007/s11099-006-0045-2

Effects of different irradiances on the photosynthetic process during ex-vitro acclimation of Anoectochilus plantlets

D. M. Pandey1,2, K. W. Yu1, R. Z. Wu1, E. J. Hahn1, K. Y. Paek1,*
1 Research Center for the Development of Advanced Horticultural Technology, Chungbuk National University, Cheong-ju, South Korea
2 Plant Molecular Biology Laboratory (Functional Genomics Lab), Department of Life Science, Sogang University, Seoul, South Korea

Six months old in vitro-grown Anoectochilus formosanus plantlets were transferred to ex-vitro acclimation under low irradiance, LI [60 µmol(photon) m-2 s-1], intermediate irradiance, II [180 µmol(photon) m-2 s-1], and high irradiance, HI [300 µmol(photon) m-2 s-1] for 30 d. Imposition of II led to a significant increase of chlorophyll (Chl) b content, rates of net photosynthesis (P N) and transpiration (E), stomatal conductance (g s), electron transfer rate (ETR), quantum yield of electron transport from water through photosystem 2 (ΦPS2), and activity of ribulose-1,5-bisphosphate carboxylase/ oxygenase (RuBPCO, EC 4.1.1.39). This indicates that Anoectochilus was better acclimated at II compared to LI treatment. On the other hand, HI acclimation led to a significant reduction of Chl a and b, P N, E, g s, photochemical quenching, dark-adapted quantum efficiency of open PS2 centres (Fv/Fm), probability of an absorbed photon reaching an open PS2 reaction centre (Fv'/Fm'), ETR, ΦPS2, and energy efficiency of CO2 fixation (ΦCO2/ΦPS2). This indicates that HI treatment considerably exceeded the photo-protective capacity and Anoectochilus suffered HI induced damage to the photosynthetic apparatus. Imposition of HI significantly increased the contents of antheraxanthin and zeaxanthin (ZEA), non-photochemical quenching, and conversion of violaxanthin to ZEA. Thus Anoectochilus modifies its system to dissipate excess excitation energy and to protect the photosynthetic machinery.

Additional key words: chlorophyll a fluorescence; electron transfer rate; photoinhibition; photosystem 2; ribulose-1,5-bisphosphate carboxylase, oxygenase; stomatal conductance; transpiration; xanthophylls

Received: September 5, 2005; Accepted: January 5, 2006; Published: September 1, 2006  Show citation

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Pandey, D.M., Yu, K.W., Wu, R.Z., Hahn, E.J., & Paek, K.Y. (2006). Effects of different irradiances on the photosynthetic process during ex-vitro acclimation of Anoectochilus plantlets. Photosynthetica, 44(3), 419-424. doi: 10.1007/s11099-006-0045-2
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