Photosynthetica 2006, 44(3):464-466 | DOI: 10.1007/s11099-006-0051-4

Localisation of CP43 in single molecules of photosystem 2 using protein deletion and scanning tunnelling microscopy

C. S. Barton1, P. B. Lukins1
1 School of Physics A28, University of Sydney, Australia

Scanning tunnelling microscopy of intact D1/D2/CP47/CP43 photosystem 2 (PS2) core complexes and CP43-deleted D1/D2/CP47 core complexes shows definitively that the CP43 subunits reside at the ends of the dimeric core complex. The CP43-removal procedure produces CP43-deleted cores with minimal conformational distortion to the D1/D2/CP47 residual core complex. There was excellent agreement between the X-ray and STM structures for the intact core complex, and between the STM image for the CP43-deleted core complex and the X-ray model with the components assigned to CP43 omitted.

Additional key words: CP43 deletion; model; STM; X-ray structure

Received: May 10, 2005; Accepted: October 24, 2005; Published: September 1, 2006  Show citation

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Barton, C.S., & Lukins, P.B. (2006). Localisation of CP43 in single molecules of photosystem 2 using protein deletion and scanning tunnelling microscopy. Photosynthetica, 44(3), 464-466. doi: 10.1007/s11099-006-0051-4
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