Photosynthetica 2007, 45(1):23 | DOI: 10.1007/s11099-007-0004-6

Short-term responses of photosynthetic membrane lipids and photochemical efficiency in plants of Phaseolus vulgaris and Vigna unguiculata submitted to high irradiance

L. C. S. Ferreira1, R. Bressan-Smith1, T. F. Elias1, F. F. Silva1, L. H. Viana1, J. G. Oliveira1
1 Setor de Fisiologia Vegetal, LMGV/CCTA, Universidade Estadual do Norte Fluminense, Campos dos Goytacazes, RJ, Brazil

Primary leaves of young plants of common bean (Phaseolus vulgaris cv. Carioca and Negro Huasteco) and cowpea (Vigna unguiculata Walp cv. Epace 10) were exposed to high irradiance (HI) of 2 000 µmol m-2 s-1 for 10, 20, and 30 min. The initial fluorescence (F0) was nearly constant in response to HI in each genotype except for Carioca. A distinct reduction of maximum fluorescence (Fm) was clearly observed in stressed genotypes of beans after 20 min followed by a slight recovery for the longer stress times. In common bean, the maximum quantum yield (Fv/Fm) was reduced slowly from 10 to 30 min of HI. In cowpea, only a slight reduction of Fv/Fm was observed at 20 min followed by recovery to normal values at 30 min. HI resulted in changes in the photochemical (qP) and non-photochemical (qN) quenching in both species, but to a different extent. In cowpea plants, more efficiency in the use of the absorbed energy under photoinhibitory conditions was related to increase in qP and decrease in qN. In addition, lipid peroxidation changed significantly in common bean genotypes with an evident increase after 20 min of HI. Hence the photosynthetic apparatus of cowpea was more tolerant to HI than that of common bean and the integrity of cowpea cell membranes was apparently maintained under HI.

Additional key words: chlorophyll a fluorescence; cultivar differences; French bean; lipid peroxidation; photoinhibition; photosynthesis; species differences

Received: May 10, 2006; Accepted: June 12, 2006; Published: March 1, 2007  Show citation

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Ferreira, L.C.S., Bressan-Smith, R., Elias, T.F., Silva, F.F., Viana, L.H., & Oliveira, J.G. (2007). Short-term responses of photosynthetic membrane lipids and photochemical efficiency in plants of Phaseolus vulgaris and Vigna unguiculata submitted to high irradiance. Photosynthetica, 45(1), 23. doi: 10.1007/s11099-007-0004-6
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    References

    1. Aro, E.-M., Virgin, I., Andersson, B.: Photoinhibition of Photosystem II. Inactivation, protein damage and turnover.-Biochim. biophys. Acta 1143: 113-134, 1993. Go to original source...
    2. Bolhàr-Nordenkampf, H.R., Long, S.P., Baker, N.R., Öquist, G., Schreiber, U., Lechner, E.G.: Chlorophyll fluorescence as a probe of the photosynthetic competence of leaves in the field: a review of current instrumentation.-Funct. Ecol. 3: 497-514, 1989. Go to original source...
    3. Buege, J.A., Aust, S.D.: Microsomal lipid peroxidation.-Methods Enzymol. 52: 302-310, 1978. Go to original source...
    4. Choudhury, N.K., Behera, R.K.: Photoinhibition of photosynthesis: Role of carotenoids in photoprotection of chloroplast constituents.-Photosynthetica 39: 481-488, 2001. Go to original source...
    5. Costa, E.S., Bressan-Smith, R., Oliveira, J.G., Campostrini, E., Pimentel, C.: Photochemical efficiency in bean plants during recovery from high temperature stress.-Braz. J. Plant Physiol. 14: 105-110, 2002. Go to original source...
    6. Dhindsa, R.S., Plumb-Dhindsa, P., Thorpe, T.A.: Leaf senescence: correlated with increased levels of membrane permeability and lipid peroxidation, and decreased levels of superoxide dismutase and catalase.-J. exp. Bot. 32: 93-101, 1981. Go to original source...
    7. Foyer, C.H., Lelandais, M., Kunert, K.J.: Photooxidative stress in plants.-Physiol. Plant. 92: 696-717, 1994. Go to original source...
    8. Gilmore, A.M., Govindjee: How higher plants respond to excess light: Energy dissipation in photosystem II.-In: Singhal, G.S., Renger, G., Sopory, S.K., Irrgang, K.-D., Govindjee (ed.): Concepts in Photobiology. Pp. 513-548. Kluwer Academic Publ., Boston-Dordrecht-London; Narosa Publishing House, Delhi-Madras-Bombay-Calcutta-London 1999. Go to original source...
    9. Greer, D.H., Berry, J.A., Björkman, O.: Photoinhibition of photosynthesis in intact bean leaves: role of light and temperature, and requirement for chloroplast-protein synthesis during recovery.-Planta 168: 253-260, 1986. Go to original source...
    10. Hideg, É., Murata, N.: The irreversible photoinhibition of the photosystem II complex in leaves of Vicia faba under strong light.-Plant Sci. 130: 151-158, 1997. Go to original source...
    11. Horton, P., Ruban, A.V., Walter, R.G.: Regulation of light harvesting in green plants.-Annu. Rev. Plant Physiol. Plant mol. Biol. 47: 655-684, 1996. Go to original source...
    12. Ivanov, A.G., Morgan, R.M., Gray, G.R., Velitchkova, M.Y., Huner, N.P.A.: Temperature/light dependent development of selective resistance to photoinhibition of photosystem I.-FEBS Lett. 430: 288-292, 1998. Go to original source...
    13. Karim, A., Fukamachi, H., Hidaka, T.: Photosynthetic performance of Vigna radiata L. leaves developed at different temperature and irradiance levels.-Plant Sci. 164: 451-458, 2003. Go to original source...
    14. Krause, G.H.: Photoinhibition of photosynthesis. An evaluation of damaging and protective mechanisms.-Physiol. Plant. 74: 566-574, 1988. Go to original source...
    15. Krause, G.H., Weis, E.: Chlorophyll fluorescence as a tool in plant physiology. II. Interpretation of fluorescence signals.-Photosynth. Res. 5: 139-157, 1984. Go to original source...
    16. Laing, W.A., Greer, D.H., Schnell, T.A.: Photoinhibition of photosynthesis causes a reduction in vegetative growth rates of dwarf bean (Phaseolus vulgaris) plants.-Aust. J. Plant Physiol. 22: 511-520, 1995. Go to original source...
    17. Lauriano, J.A., Lidon, F.C., Carvalho, C.A., Campos, P.S., do Céu Matos, M.: Drought effects on membrane lipids and photosynthetic activity in different peanut cultivars.-Photosynthetica 38: 7-12, 2000. Go to original source...
    18. Long, S.P., Humphries, S., Falkowski, P.G.: Photoinhibition of photosynthesis in nature.-Annu. Rev. Plant Physiol. Plant mol. Biol. 45: 633-662, 1994. Go to original source...
    19. Macpherson, A.N., Telfer, A., Barber, J., Truscott, T.G.: Direct detection of singlet oxygen from isolated photosystem II reaction centres.-Biochim. biophys. Acta 1143: 301-309, 1993. Go to original source...
    20. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide.-J. exp. Bot. 345: 659-668, 2000. Go to original source...
    21. Mishra, N.P., Ghanotakis, D.F.: Exposure of a photosystem II complex to chemically generated singlet oxygen results in D1 fragments similar to the ones observed during aerobic photoinhibition.-Biochim. biophys. Acta 1187: 296-300, 1994. Go to original source...
    22. Mittler, R.: Oxidative stress, antioxidants and stress tolerance.-Trends Plant Sci. 9: 405-410, 2002. Go to original source...
    23. Nedbal, L., Masojídek, J., Komenda, J., Prá¹il, O., ©etlík, I.: Three types of photosystem II photoinactivation. 2. Slow processes.-Photosynth. Res. 24: 89-97, 1990. Go to original source...
    24. Ohad, I., Kyle, D.J., Arntzen, C.J.: Membrane protein damage and repair. II. Removal and replacement of inactivated 32-kilodalton polypeptides in chloroplast membranes.-J. Cell Biol. 99: 481-485, 1984. Go to original source...
    25. Powles, S.B., Berry, J.A., Björkman, O.: Interaction between light and chilling temperature on the inhibition of photosynthesis in chilling-sensitive plants.-Plant Cell Environ. 6: 117-123, 1983. Go to original source...
    26. Scandalios, J.G.: Oxygen stress and superoxide dismutases.-Plant Physiol. 101: 7-12, 1993. Go to original source...
    27. Schansker, G., van Rensen, J.J.S.: Performance of active photosystem II centers in photoinhibited pea leaves.-Photosynth. Res. 62: 175-184, 1999. Go to original source...
    28. Schreiber, U., Bilger, W., Neubauer, C.: Chlorophyll fluorescence as a nonintrusive indicator for rapid assessment of in vivo photosynthesis.-In: Schulze, E.-D., Caldwell, M.M. (ed.): Ecophysiology of Photosynthesis. Pp. 49-70. Springer-Verlag, Berlin 1994. Go to original source...
    29. Schuster, G., Timberg, R., Ohad, I.: Turnover of thylakoid photosystem II proteins during photoinhibition of Chlamydomonas reinhardtii.-Eur. J. Biochem. 177: 403-410, 1988. Go to original source...
    30. Silva, F.F.: Photochemical Efficiency, Radiation Use Efficiency and Productivity in Bean (Phaseolus vulgaris and Vigna unguiculata) Cultivated in Summer and Winter, in Rio de Janeiro State, Brazil.-MS Thesis. Universidade Estadual do Norte Fluminense, Campos dos Goytacazes 2001.
    31. Tsonev, T., Velikova, V., Georgieva, K., Hyde, P.F., Jones, H.G.: Low temperature enhances photosynthetic down-regulation in French bean (Phaseolus vulgaris L.) plants.-Ann. Bot. 91: 343-352, 2003. Go to original source...
    32. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. The peroxidative destruction of lipids during chilling injury to photosynthesis and ultrastructure.-Plant Physiol. 83: 272-277, 1987a. Go to original source...
    33. Wise, R.R., Naylor, A.W.: Chilling-enhanced photooxidation. Evidence for the role of singlet oxygen and superoxide in the breakdown of pigments and endogenous antioxidants.-Plant Physiol. 83: 278-282, 1987b. Go to original source...
    34. Yordanov, I., Tsonev, T., Goltsev, V., Kruleva, L., Velikova, V.: Interactive effect of water deficit and high temperature on photosynthesis in sunflower and maize plants. 1. Changes in parameters of chlorophyll fluorescence induction kinetics and fluorescence quenching.-Photosynthetica 33: 391-402, 1997.

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