Photosynthetica 2007, 45(1):105-109 | DOI: 10.1007/s11099-007-0016-2

Irradiation-induced in vivo re-localization of NADPH-protochlorophyllide oxidoreductase from prolamellar body to stroma of barley etioplast

D. Kovacevic1, D. Dewez1, R. Popovic1,*
1 Département de Chimie et de Biochimie, Centre TOXEN, Université du Québec à Montréal, Montréal, Canada

Distribution of NADPH-protochlorophyllide oxidoreductase (POR) in etioplast of etiolated barley leaf was studied by using Western blot analyses of etioplast fractions isolated on a sucrose gradient. When the leaf was exposed to light, POR content decreased in the etioplast inner membrane and prolamellar body sub-membrane fraction while it was simultaneously increased in the stroma. By using 77 K fluorescence spectroscopy analyzes, we found for irradiated etiolated leaf that the POR protein in the stroma was co-localized with chlorophyllide (Chlide) emitting at 678 nm. Relocalization of the POR-Chlide complex induced by irradiation suggests that POR participates in the pigment transport processes during early stages of the thylakoid membrane development.

Additional key words: chlorophyll(ide); chloroplast development; envelope; fluorescence spectra; Hordeum vulgare; prothylakoid

Received: February 17, 2006; Accepted: July 20, 2006; Published: March 1, 2007  Show citation

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Kovacevic, D., Dewez, D., & Popovic, R. (2007). Irradiation-induced in vivo re-localization of NADPH-protochlorophyllide oxidoreductase from prolamellar body to stroma of barley etioplast. Photosynthetica, 45(1), 105-109. doi: 10.1007/s11099-007-0016-2
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