Photosynthetica 2007, 45(2):317-320 | DOI: 10.1007/s11099-007-0054-9

Photosynthetic characteristics of hybrid rice with phosphoenolpyruvate carboxylase gene

L. Ling1, B. J. Zhang2, D. M. Jiao3,*
1 College of Life Science, Sichuan University, Chengdu, China
2 Department of Life Science, Xiaozhuang College, Nanjing, China
3 84-401, Jiangsu Academy of Agricultural Sciences, Nanjing, China

High level of phosphoenolpyruvate carboxylase (PEPC) gene was stably inherited and transferred from the male parent, PEPC transgenic rice, into a female parent, japonica rice cv. 9516. Relative to the female parent, the produced JAAS45 pollen lines exhibited high PEPC activity (17-fold increase) and also higher photosynthetic rates (about 36 %-fold increase). The JAAS45 pollen lines were more tolerant to photoinhibition and to photo-oxidative stress. Furthermore, JAAS45 pollen lines, as well as their male parent, were tested to exhibit a limiting C4 cycle by feeding with exogenous C4 primary products such as oxaloacetate (OAA). Thus the PEPC gene and photosynthetic characteristics of PEPC transgenic rice could be stably transferred to the hybrid progenies, which might open a new breeding approach to the integration of conventional hybridization and biological technology.

Additional key words: anther culture; C4 cycle; chlorophyll fluorescence induction; net photosynthetic rate; Oryza; oxaloacetate; photosystem 2; transgenic plants; Zea

Received: July 11, 2006; Accepted: January 15, 2007; Published: June 1, 2007  Show citation

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Ling, L., Zhang, B.J., & Jiao, D.M. (2007). Photosynthetic characteristics of hybrid rice with phosphoenolpyruvate carboxylase gene. Photosynthetica, 45(2), 317-320. doi: 10.1007/s11099-007-0054-9
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    References

    1. Chi, W., Jiao, D.-M., Li, X., Kuang, T.-Y., Ku, M.S.B.: Photosynthetic characteristics of transgenic rice plants overexpressing maize phosphoenolpyruvate carboxylase.-Acta bot. sin. 43: 657-660, 2001.
    2. Fukayama, H., Agarie, S., Nomura, M., Tsuchida, H., Ku, M.S.B.: High-level expression of maize C4-specific pyruvate, Pi dikinase and its light activation in transgenic rice plants.-Plant Cell Physiol. 40: s116, 1999.
    3. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    4. Gonzalez, D.H., Iglesias, A.A., Andreo, C.S.: On the regulation of phosphoenolpyruvate carboxylase activity from maize leaves by L-malate. Effect of pH.-J. Plant Physiol. 116: 425-434, 1984. Go to original source...
    5. Huang, X.Q., Jiao, D.M., Chi, W., Ku, M.S.B.: Characteristics of CO2 exchange and chlorophyll fluorescence of transgenic rice with C4 genes.-Acta bot. sin. 44: 405-412, 2002.
    6. Ji, B.H., Zhu, S.Q., Jiao, D.M.: A limited photosynthetic C4-microcycle and its physiological function in transgenic rice plant expressing the maize PEPC gene.-Acta bot. sin. 46: 542-551, 2004.
    7. Jiao, D., Huang, X., Li, X., Chi, W., Kuang, T., Zhang, Q., Ku, M.S.B., Cho, D.: Photosynthetic characteristics and tolerance to photo-oxidation of transgenic rice expressing C4 photosynthesis enzymes.-Photosynth. Res. 72: 85-93, 2002. Go to original source...
    8. Jiao, D.M., Kuang, T.Y., Li, X., Ge, Q.Y., Huang, X.Q., Hao, N.B., Bai, K.Z.: Physiological characteristics of the primitive CO2 concentrating mechanism in PEPC transgenic rice.-Sci. China C 46: 438-446, 2003. Go to original source...
    9. Jiao, D.M., Li, X., Ji, B.H.: Photoprotective effects of high level expression of C4 phosphoenolpyruvate carboxylase in transgenic rice during photoinhibition.-Photosynthetica 43: 501-508, 2005. Go to original source...
    10. Ku, M.S.B., Agarie, S., Nomura, M., Fukayama, H., Tsuchida, H., Ono, K., Hirose, S., Toki, S., Miyao, M., Matsuoka, M.: High level expression of maize phosphoenolpyruvate carboxylase in transgenic rice plants.-Nat. Biotechnol. 17: 76-80, 1999. Go to original source...
    11. Lin, Z.F., Peng, C.L., Lin, G.Z.: [Diurnal changes of chlorophyll fluorescence quenching and the response to photooxidation in leaves of C3 and C4 plants.]-Acta agron. sin. 25: 284-290, 1999. [In Chin.]
    12. Takeuchi, Y., Akagi, H., Kamasawa, N., Osumi, M., Honda, H.: Aberrant chloroplasts in transgenic rice plants expressing a high level of maize NADP-dependent malic enzyme.-Planta 211: 265-274, 2000. Go to original source...
    13. Wang, D.Z., Jiao, D.M., Wu, S., Li, X., Li, L., Chi, W., Wang, S.H., Li, C.Q., Luo, Y.C., Wang, X.F.: [Breeding for parents of hybrid rice with maize pepc gene.]-Sci. Agr. sin. 35: 1165-1170, 2002. [In Chin.]
    14. Wang, D.Z., Wang, S.H., Wu, S., Li, C.Q., Jiao, D.M., Luo, Y.C., Wang, X.F., Du, S.Y.: [Inheritance and expression of the maize pepc gene in progenies of transgenic rice bred by crossing.]-Acta genet. sin. 31: 195-201, 2004. [In Chin.]
    15. Wang, Z., Fang, X.J.: [Plant DNA isolation.]-Mol. Plant Breed. 1: 281-288, 2003. [In Chin.]

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