Photosynthetica 2008, 46(3):441-451 | DOI: 10.1007/s11099-008-0076-y

Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants

M. Pineda1, J. Soukupová2,3, K. Matou¹2,3, L. Nedbal2,3, M. Barón1,*
1 Department of Biochemistry, Molecular and Cell Biology of Plants, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas (CSIC), Granada, Spain
2 Institute of Systems Biology and Ecology, Academy of Sciences of the Czech Republic, Nové Hrady, Czech Republic
3 Institute of Physical Biology, University of South Bohemia, Nové Hrady, Czech Republic

We compared by chlorophyll (Chl) fluorescence imaging the effects of two strains of the same virus (Italian and Spanish strains of the Pepper mild mottle virus - PMMoV-I and-S, respectively) in the host plant Nicotiana benthamiana. The infection was visualized either using conventional Chl fluorescence parameters or by an advanced statistical approach, yielding a combinatorial set of images that enhances the contrast between control and PMMoV-infected plants in the early infection steps. Among the conventional Chl fluorescence parameters, the non-photochemical quenching parameter NPQ was found to be an effective PMMoV infection reporter in asymptomatic leaves of N. benthamiana, detecting an intermediate infection phase. The combinatorial imaging revealed the infection earlier than any of the standard Chl fluorescence parameters, detecting the PMMoV-S infection as soon as 4 d post-inoculation (dpi), and PMMoV-I infection at 6 dpi; the delay correlates with the lower virulence of the last viral strain.

Additional key words: biotic stress; Nicotiana benthamiana; non-photochemical quenching; Pepper mild mottle virus

Received: October 16, 2007; Accepted: May 2, 2008; Published: September 1, 2008  Show citation

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Pineda, M., Soukupová, J., Matou¹, K., Nedbal, L., & Barón, M. (2008). Conventional and combinatorial chlorophyll fluorescence imaging of tobamovirus-infected plants. Photosynthetica, 46(3), 441-451. doi: 10.1007/s11099-008-0076-y
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