Photosynthetica 2008, 46(3):466 | DOI: 10.1007/s11099-008-0080-2

Effect of Cd on growth, photosynthetic gas exchange, and chlorophyll fluorescence of wild and Cd-sensitive mutant rice

J. Y. He1,2,*, Y. F. Ren1, C. Zhu2, Y. P. Yan2, D. A. Jiang2
1 College of Agriculture, Guizhou University, Guiyang, P.R. China
2 State Key Lab of Plant Physiology and Biochemistry, College of Life Science, Zhejiang University, Hangzhou, P.R. China

Growth, photosynthetic gas exchange, and chlorophyll fluorescence characteristics were investigated in wild type (WT) and Cd-sensitive mutant rice (Oryza sativa L.) plants using 50 µM Cd treatment for 12 d followed by a 3-d recovery. Under Cd stress, net dry mass and pigment contents were significantly lower in the mutant plants than in the WT. The mutant had lower net photosynthetic rate (P N), transpiration rate (E), and stomatal conductance (g s) than WT rice, however, it had higher intercellular CO2 concentration (C i), indicating that non-stomatal factors accounted for the inhibition of P N. Maximal photochemical efficiency of photosystem 2 (Fv/Fm), effective quantum yield of PS2 (ΦPS2), and photochemical quenching (qP) decreased much in the mutant under Cd stress. Cd content in roots and leaves of the mutant was significantly higher than those in the WT. Hence Cd toxicity was associated with the marked increases in Cd contents of plant tissue. After the recovery for 3 d, the WT rice had higher capacity to recover from Cd injury than the mutant.

Additional key words: chlorophyll; growth; intercellular CO2 concentration; Oryza; recovery; stomatal conductance; transpiration rate

Received: January 24, 2008; Accepted: March 5, 2008; Published: September 1, 2008  Show citation

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He, J.Y., Ren, Y.F., Zhu, C., Yan, Y.P., & Jiang, D.A. (2008). Effect of Cd on growth, photosynthetic gas exchange, and chlorophyll fluorescence of wild and Cd-sensitive mutant rice. Photosynthetica, 46(3), 466. doi: 10.1007/s11099-008-0080-2
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    References

    1. Atal, N., Saradhi, P.P., Mohanty, P.: Inhibition of the chloroplast photochemical reactions by treatment of wheat seedlings with low concentrations of cadmium: Analysis of electron transport activities and changes in fluorescence yield.-Plant Cell Physiol. 32: 943-951, 1991. Go to original source...
    2. Burzyñski, M., K³obus, G.: Changes of photosynthetic parameters in cucumber leaves under Cu, Cd, and Pb stress.-Photosynthetica 42: 505-510, 2004. Go to original source...
    3. Burzyñski, M., Żurek, A.: Effects of copper and cadmium on photosynthesis in cucumber cotyledons.-Photosynthetica 45: 239-244, 2007. Go to original source...
    4. Chen, J., Zhu, C., Lin, D., Sun, Z.X.: The effects of Cd on lipid peroxidation, hydrogen peroxide content and antioxidant enzyme activities in Cd-sensitive mutant rice seedlings.-Can. J. Plant Sci. 87: 49-57, 2007. Go to original source...
    5. Clijsters, H., Van Assche, F.: Inhibition of photosynthesis by heavy metals.-Photosynth. Res. 7: 31-40, 1985. Go to original source...
    6. Dalla Vecchia, F., La Rocca, N., Moro, I., de Faveri, S., Andreoli, C., Rascio, N.: Morphogenetic, ultrastructural and physiological damages suffered by submerged leaves of Elodea canadensis exposed to cadmium.-Plant Sci. 168: 329-338, 2005. Go to original source...
    7. Das, P., Samantaray, S., Rout, G.R.: Studies on cadmium toxicity in plants: a review.-Environ. Pollut. 98: 29-36, 1997. Go to original source...
    8. Ernst, W.H.O.: Bioavailability of heavy metals and decontamination of soils by plants.-Appl. Geochem. 11: 163-167, 1996. Go to original source...
    9. Genty, B., Briantais, J.-M., Baker, N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence.-Biochim. biophys. Acta 990: 87-92, 1989. Go to original source...
    10. He, J.Y., Zhu, C., Ren, Y.F., Jiang, D.A., Sun, Z.X.: Root morphology and cadmium uptake kinetics of the cadmium-sensitive mutant rice.-Biol. Plant. 51: 791-794, 2007. Go to original source...
    11. Howden, R., Cobbett, C.S.: Cadmium sensitive mutant of Arabidopsis thaliana.-Plant Physiol. 99: 100-107, 1992. Go to original source...
    12. Howden, R., Goldsbrough, P.B., Anderson, C.R., Cobbett, C.S.: Cadmium sensitive, cad1 mutant of Arabidopsis thaliana are phytochelatin deficient.-Plant Physiol. 107: 1059-1066, 1995. Go to original source...
    13. Kao, W.-Y., Tsai, T.-T., Shin, C.-N.: Photosynthetic gas exchange and chlorophyll a fluorescence of three wild soybean species in response to NaCl treatments.-Photosynthetica 41: 415-419, 2003. Go to original source...
    14. Krupa, Z., Baszyñski, T.: Some aspects of heavy metals toxicity towards photosynthetic apparatus - direct and indirect effects on light and dark reactions.-Acta Physiol. Plant. 17: 177-190, 1995.
    15. Kûpper, H., Kûpper, F., Spiller, M.: Environmental relevance of heavy metal-substituted chlorophyll using the example of water plants.-J. exp. Bot. 47: 259-266, 1996.
    16. Lichtenthaler, H.K., Wellburn, A.R.: Determinations of total carotenoids and chlorophylls a and b of leaf extracts in different solvents.-Biochem. Soc. Trans. 603: 591-592, 1983. Go to original source...
    17. Linger, P., Ostwald, A., Haensler, J.: Cannabis sativa L. growing on heavy metal contaminated soil: growth, cadmium uptake and photosynthesis.-Biol. Plant. 49: 567-576, 2005. Go to original source...
    18. Maxwell, K., Johnson, G.N.: Chlorophyll fluorescence - a practical guide.-J. exp. Bot. 51: 659-668, 2000. Go to original source...
    19. Moya, J.L., Ros, R., Picazo, I.: Influence of cadmium and nickel on growth, net photosynthesis and carbohydrate distribution in rice plants.-Photosynth. Res. 36: 75-80, 1993. Go to original source...
    20. Otero, S., Núñez-Olivera, E., Martínez-Abaigar, J., Tomás, R., Arróniz-Crespo, M., Beaucourt, N.: Effects of cadmium and enhanced UV radiation on the physiology and the concentration of UV-absorbing compounds of the aquatic liverwort Jungermannia exsertifolia subsp. cordifolia.-Photochem. photobiol. Sci. 5: 760-769, 2006. Go to original source...
    21. Pál, M., Horváth, E., Janda, T., Páldi, E., Szalai, G.: Physiological changes and defence mechanisms induced by cadmium stress in maize.-J. Plant Nutr. Soil Sci. 169: 239-246, 2006. Go to original source...
    22. Prasad, M.N.V., Strzalka, K.: Impact of heavy metals on photosynthesis.-In: Prasad, M.N.V., Hagemeyer, J. (ed.): Heavy Metal Stress in Plants. Pp. 117-138. Springer, Heidelberg 1999. Go to original source...
    23. Prasad, S.M., Zeeshan, M.: UV-B radiation and cadmium induced changes in growth, photosynthesis, and antioxidant enzymes of cyanobacterium Plectonema boryanum.-Biol. Plant. 49: 229-236, 2005. Go to original source...
    24. Sanita di Toppi, L., Gabbrielli, R.: Response to cadmium in higher plants.-Environ. exp. Bot. 41: 105-130, 1999. Go to original source...
    25. Siedlecka, A., Krupa, Z.: Interaction between cadmium and iron and its effects on photosynthetic capacity of primary leaves of Phaseolus vulgaris.-Plant Physiol. Biochem. 34: 833-841, 1996.
    26. Vajpayee, R.D., Rai, U.N., Ali, M.B., Tripati, R.D., Singh, S.N.: Chromium-induced physiologic changes in Vallisneria spiralis L. and its role in hytoremediation of tannery effluents.-Bull. Environ. Contam. Toxicol. 67: 246-256, 2001. Go to original source...
    27. Vassilev, A., Manolov, P.: Chlorophyll fluorescence of barley (H. vulgare L.) seedlings grown in excess of Cd.-Bulg. J. Plant Physiol. 25: 67-76, 1999.
    28. Wickens, L.K., Cheeseman, J.M.: Application of growth analysis to physiological studies involving environmental discontinuities.-Physiol. Plant. 73: 271-277, 1988. Go to original source...
    29. Wu, F.B., Zhang, G.P., Yu, J.S.: Genotypic differences in effect of Cd on photosynthesis and chlorophyll fluorescence of barley (Hordeum vulgare L.).-Bull. Environ. Contam. Toxicol. 71: 1272-1281, 2003. Go to original source...
    30. Zhu, Z.G., Fu, Y.P., Xiao, H., Hu, G.C., Yu, Y.H., Si, H.M., Zhang, J.L., Sun, Z.X.: [Construction of rice mutant pool inserted the maize transposable element Ac/Ds and genetic analysis for several mutants.]-Chin. J. Biotechnol. 17: 288-292, 2001. [In Chin.]

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