Photosynthetica 2009, 47(3):409 | DOI: 10.1007/s11099-009-0062-z

Content of carotenoids during ageing and senescence of tobacco leaves with genetically modulated life-span

D. Procházková1,*, D. Haisel1, N. Wilhelmová1
1 Institute of Experimental Botany, Academy of Sciences of the Czech Republic, Prague 6, Czech Republic

We exploited leaves of tobacco (Nicotiana tabacum L., cv. Wisconsin 38) with introduced chimeric construct consisting of SAG12 promoter fused with ipt gene for cytokinin synthesis and therefore prolonged life-span. As a control we used its wild type. In 12-week-old plants, the first leaves of control plants showed senescence symptoms at the time of sampling. Carotenoid content decreased with increasing leaf age both in control and in transgenic plants. On the other hand, the first leaves of transgenic plants demonstrated better antioxidant capacity represented by carotenoids compared to the leaves of control plants of the same age. They stayed still green at this age.

Additional key words: cytokinin; reactive oxygen species; xanthophylls

Received: March 16, 2009; Accepted: August 18, 2009; Published: September 1, 2009  Show citation

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Procházková, D., Haisel, D., & Wilhelmová, N. (2009). Content of carotenoids during ageing and senescence of tobacco leaves with genetically modulated life-span. Photosynthetica, 47(3), 409. doi: 10.1007/s11099-009-0062-z
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