Photosynthetica 2014, 52(3):404-412 | DOI: 10.1007/s11099-014-0047-4

Water stress and abscisic acid treatments induce the CAM pathway in the epiphytic fern Vittaria lineata (L.) Smith

B. D. Minardi1,*, A. P. L. Voytena1, M. Santos1, Á. M. Randi1
1 Plant Physiology Laboratory, Department of Botany, Federal University of Santa Catarina, Florianópolis, Brazil

Among various epiphytic ferns found in the Brazilian Atlantic Forest, we studied Vittaria lineata (L.) Smith (Polypodiopsida, Pteridaceae). Anatomical characterization of the leaf was carried out by light microscopy, fluorescence microscopy, and scanning electron microscopy. V. lineata possesses succulent leaves with two longitudinal furrows on the abaxial surface. We observed abundant stomata inside the furrows, glandular trichomes, paraphises, and sporangia. We examined malate concentrations in leaves, relative water content (RWC), photosynthetic pigments, and chlorophyll (Chl) a fluorescence in control, water-deficient, and abscisic acid (ABA)-treated plants. Plants subjected to drought stress (DS) and treated by exogenous ABA showed significant increase in the malate concentration, demonstrating nocturnal acidification. These findings suggest that V. lineata could change its mode of carbon fixation from C3 to the CAM pathway in response to drought. No significant changes in RWC were observed among treatments. Moreover, although plants subjected to stress treatments showed a significant decline in the contents of Chl a and b, the concentrations of carotenoids were stable. Photosynthetic parameters obtained from rapid light curves showed a significant decrease after DS and ABA treatments.

Additional key words: chlorophyll fluorescence; malate; morphoanatomy; photosynthetic pathway; pigments

Received: June 3, 2013; Accepted: December 17, 2013; Published: September 1, 2014  Show citation

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Minardi, B.D., Voytena, A.P.L., Santos, M., & Randi, Á.M. (2014). Water stress and abscisic acid treatments induce the CAM pathway in the epiphytic fern Vittaria lineata (L.) Smith. Photosynthetica, 52(3), 404-412. doi: 10.1007/s11099-014-0047-4
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    References

    1. Ashraf, M.Y., Azmi, A.R., Khan, A.H., Ala, S.A.: Effect of water stress on total phenols, peroxidase activity and chlorophyll content in wheat (Triticum aestivum L.). - Acta Physiol. Plant. 16: 185-191, 1994.
    2. Barrs, H.D., Weatherley, P.E.: A re-examination of the relative turgidity technique for estimating water deficits in leaves. - Aust. J. Biol. Sci. 15: 413-428, 1962. Go to original source...
    3. Bennet, B.C.: Patchiness, diversity and abundance relationships of vascular epiphytes. - Selbyana 9: 70-75, 1986.
    4. Benzing, D.H.: The vegetative basis of vascular epiphytism. - Selbyana 9: 23-43, 1986.
    5. Benzing, D.H.: Vascular Epiphytes. Pp. 376. Cambridge Univ. Press, Cambridge 1990. Go to original source...
    6. Bjorkman, O., Demmig, B.: Photon yield of O2 evolution and chlorophyll fluorescence characteristics at 77-K among vascular plants of diverse origins. - Planta 170: 489-504, 1987. Go to original source...
    7. Bukatsch, F.: [Observations of double staining Astra bluesafranin.] - Mikrokosmos 61: 255, 1972. [In German]
    8. Brodribb, T.J., McAdam, S.A.M.: Passive origins of stomata control in vascular plants. - Science 331: 582-585, 2011. Go to original source...
    9. Carter, J.P., Martin, C.E.: The occurrence of Crassulacean acid metabolism among epiphytes in a high-rainfall region of Costa Rica. - Selbyana 15: 104-106, 1994.
    10. Chu, C., Dai, Z.Y., Ku, M. S. B., Edwards, G. E.: Induction of Crassulacean acid metabolism in the facultative halophyte Mesembryanthemum crystallinum by abscisic acid. - Plant. Physiol. 93: 1253-1260, 1990. Go to original source...
    11. Costa, A. F.: [Pharmacognosy. Vol. 2.] Pp. 755. Fundação Calouste Gulbenkian, Lisboa 1982. [In Portuguese]
    12. Constable, J. V. H., Grace, J. B., Longstreth, D. J.: High carbon dioxide concentrations in aerenchyma of Typha latifolia. - Am. J. Bot. 79: 415-418. 1992. Go to original source...
    13. Cushman, J. C., Borland, A. M.: Induction of Crassulacean acid metabolism by water limitation. - Plant Cell Environ. 25: 295-310, 2002. Go to original source...
    14. Drew, M. C., He, C.J., Morgan, P. W.: Programmed cell death and aerenchyma formation in roots. - Trends Plant Sci. 5: 123-127, 2000. Go to original source...
    15. Freschi, L., Rodrigues, M. A., Tiné, M. A. S., Mercier, H.: Correlation between citric acid and nitrate metabolisms during CAM cycle in the atmospheric bromeliad Tillandsia pohliana. - J. Plant Physiol. 167: 1577-1583, 2010. Go to original source...
    16. Genty, B., Briantais, J. M., Baker, N. R. The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - Biochim. Biophys. Acta. 900: 87-92, 1989. Go to original source...
    17. Gerlach, D.: [Botanical Microtechnology: An Introduction.] Pp. 311. Georg Thieme Verlag, Stuttgart 1984. [In German]
    18. Gloag, R. S., Ritchie, R. J., Chen, M. et al.: Chromatic photoacclimation, photosynthetic electron transport and oxygen evolution in the chlorophyll d-containing oxyphotobacterium Acaryochloris marina. - Biochim. Biophys. Acta 1767: 127-135, 2007. Go to original source...
    19. Griffiths, H., Ong, B. L., Avadhani, P. N., Goh, C. J.: Recycling of respiratory CO2 during Crassulacean acid metabolism: alleviation of photoinhibition in Pyrrosia piloselloides. - Planta 179: 115-122, 1989. Go to original source...
    20. Hew, C. S., Wong, Y. S., Photosynthesis and respiration of ferns in relation to their habitat. - Am. Fern J. 64: 40-48, 1974. Go to original source...
    21. Hietz, P., Briones, O.: Correlation between water relations and within-canopy distribution of epiphytic ferns in a Mexican cloud forest. - Oecologia 114: 305-316, 1998. Go to original source...
    22. Hoagland, D. R., Arnon D.I.: The water culture method for growing plants without soil. - Calif. Exp. Stn. Circ. 347: 1-39, 1938.
    23. Horton, R. F.: Stomatal opening: the role of abscisic acid. - Can. J. Bot. 49: 583-585, 1971. Go to original source...
    24. Jaing, Y., Yang, W. Y., Jiang, X., Qiaoyon, C.: Active oxygen demand and effect on chlorophyll degradation in rice seedling under osmotic stress. - Acta Bot. Sin. 36: 289-295, 1994.
    25. Jensen, W. A.: Botanical Histochemistry: Principles and Practice. Pp. 408. W. H. Freeman & Co, San Francisco 1962.
    26. Johansen, D. A.: Plant Microtechnique. Pp. 523. McGraw Hill Book, New York 1940.
    27. Kraus, J.E, Arduin, M.: [Basic Manual of Methods in Plant Morphology.] Pp. 198. Editora Universidade Rural. Seropédica, 1997. [In Portuguese]
    28. Kluge, M., Avadhani, P. N., Goh, C. J.: Gas exchange and water relations in epiphytic tropical ferns. - In: Lüttge U. (ed.): Vascular Plants as Epiphytes. Ecological Studies 76. Pp. 87-109. Spriger-Verlag, Berlin 1989. Go to original source...
    29. Kress, W. J.: A symposium: the biology of tropical epiphytes. - Selbyana 9: 1-22, 1986.
    30. Lichtenthaler, H. K.: Chlorophylls and carotenoids: pigments of photosynthetic biomembranes. - Method. Enzymol. 148: 350-382, 1987. Go to original source...
    31. Lüttge, U.: The role of crassulacean acid metabolism (CAM) in the adaptation of plants to salinity. - New Phytol. 125: 59-71, 1993. Go to original source...
    32. Madison, M.: Vascular epiphytes: their systematic occurrence and salient features. - Selbyana 2: 1-13, 1977.
    33. Martin, S. L., Davis, R., Protti, P. et al.: The occurrence of crassulacean acid metabolism in epiphytic ferns, with an emphasis on the Vittariaceae. - Int. J. Plant Sci. 166: 623-630, 2005. Go to original source...
    34. Maxwell, K., Johnson, G. N.: Chlorophyll fluorescence: A practical guide. - J. Exp. Bot. 51: 659-668, 2000. Go to original source...
    35. McAdam, S. A. M., Brodribb, T. J.: Stomatal innovation and the rise of seed plants. - Ecol. Lett. 15: 1-8, 2012. Go to original source...
    36. Möllering, H.: L(-) malate. - In: Bergmeyer, H.U. (ed.): Methods of Enzymatic Analysis. Pp. 39-47, Vol 7. VHC Verlagsgesellschaft, Weinheim 1985.
    37. Neales, T. F, Hew, C. S.: Two types of carbon fixation in tropical orchids. - Planta 23: 303-306, 1975. Go to original source...
    38. Nieder, J. Prosperí, J., Michaloud, G.: Epiphytes and their contribution to canopy diversity. - Plant Ecol. 153: 51-63, 2001. Go to original source...
    39. Nimmo, H. G.: The regulation of phosphoenolpyruvate carboxylase in CAM plants. - Trends Plant Sci. 5: 75-80, 2000. Go to original source...
    40. N'soukpoé-Kossi, C.N., Ivanov, A.G., Veeranjaneyulu, K., Leblanc, R. M. Protective action of abscisic acid against the inhibition of photosynthesis of barley leaves by bisulphite. - Photosynthetica 36: 51-60, 1999. Go to original source...
    41. O'Brien, T. P., Feder, N., McCully, M.: Polychromatic staining of plant cell walls by toluidine blue. - Protoplasma 59: 368-373, 1965. Go to original source...
    42. Pedersen, O., Sand-Jensen, K.: Adaptations of submerged Lobelia dortmanna to aerial life form: morphology, carbon sources, and oxygen dynamics. - Oikos 65: 89-96, 1992. Go to original source...
    43. Peltzer, D., Dreyer, E., Polle, A.: Differential temperature dependencies of antioxidative enzymes in two contrasting species: Fagus sylvatica and Coleus blumei. - Plant Physiol. Bioch. 40: 141-150, 2002. Go to original source...
    44. Pinhero, R. G., Rao, M. V., Paliyath, G. et al.: Changes in activities of antioxidant enzymes and their relationship to genetic and paclobutrazol-induced chilling tolerance of maize seedlings. - Plant Physiol. 114: 695-704, 1997. Go to original source...
    45. Prado, J.: [Reviews and monographs as a basis for analysis of diversity, as we know about our flora.] - In: Jardim, M. A. G., Bastos, M. N. C., Santos, J. U. M. (ed.): [Challenges of Brazilian Botany in the new Millennium: Inventory, Systematic and Conservation of the Plant Diversity.] Pp. 78-79. MPEG/UFRA/EMBRAPA, Belém 2003. [In Portuguese]
    46. Pryer, K. M., Schuettpelz, E., Wolf, P. G. et al.: Phylogeny and evolution of ferns (Monilophytes) with a focus on the early leptosporangiate divergences. - Am. J. Bot. 91: 1582-1598, 2004. Go to original source...
    47. Ralph, P. J., Gademann, R.: Rapid light curves: a powerful tool to assess photosynthetic activity. - Aquat. Bot. 82: 222-237, 2005. Go to original source...
    48. Rathinasabapathi, B.: Ferns represent untapped biodiversity for improving crops for enviromental stress tolerance. - New Phytol. 172: 385-390, 2006. Go to original source...
    49. Ravensberg, W. J., Hennipman, E.: The Pyrrosia species formerly referred to as Drymoglossum and Saxiglossum. - Leiden Bot. Ser. 9: 281-310, 1986.
    50. Reddy, A. R., Chaitanya, K. V., Vivekanandan, M.: Droughtinduced responses of photosynthesis and antioxidant metabolism in higher plants. - J. Plant Physiol. 161: 1189-1202, 2004. Go to original source...
    51. Ritchie, R. J.: Fitting light saturation curves measured using modulated fluorometry. - Photosynth. Res. 96: 201-215, 2008. Go to original source...
    52. Runcie, J. W., Durako, M. J.: Among-shoot variability and leafspecific absorptance characteristics affect diel estimates of in situ electron transport of Posidonia australis. - Aquat. Bot. 80: 209-220, 2004. Go to original source...
    53. Rut, G., Krupa, J., Miszalski, Z. et al.: Crassulacean acid metabolism in the epiphytic fern Platycerium bifurcatum. - Photosynthetica 46: 156-160, 2008. Go to original source...
    54. Ruzin, S. E.: Plant Microtechnique and Microscopy. Pp. 322-322. Oxford University Press, New York 1999.
    55. Schreiber, U.: Pulse-amplitude (PAM) fluorometry and saturation pulse method. - In: Papageorgiou, G. and Govindjee (ed.): Chlorophyll a Fluorescence: a Signature of Photosynthesis. Advances in Photosynthesis and Respiration Series. Pp. 279-319. Kluwer Academic Publishers, Dordrecht 1994. Go to original source...
    56. Smith, A. R., Pryer, K. M., Schuettpelz, E., Korall, P., Schneider, H., Wolf, P. G. A classification for extant ferns. - Taxon 55: 705-731, 2006. Go to original source...
    57. Sinclair, R.: Water relations of tropical epiphytes. III. Evidence for crassulacean acid metabolism. - J. Exp. Bot. 35: 1-7, 1984. Go to original source...
    58. Tausz, M., Hietz, P., Briones, O.: The significance of carotenoids and tocopherols in photoprotection of seven epiphytic fern species of a Mexican cloud forest. - Aust. J. Plant Physiol. 28: 775-783, 2001. Go to original source...
    59. Tryon, R. M., Tryon, A.F.: Ferns and Allied Plants with Special Reference to Tropical America. Pp. 857. Springer-Verlag, New York 1982. Go to original source...
    60. Uphof, J. C. T.: Physiological anatomy of xerophytic Selaginellas. - New Phytol. 19: 101-131, 1920. Go to original source...
    61. White, A. J., Critchley, C.: Rapid light curves: a new fluorescence method to assess the state of the photosynthetic apparatus. - Photosynth. Res. 59: 63-72, 1999. Go to original source...
    62. Winter, K., Wallace, B. J., Stocker, G. C., Roksandic, Z.: Crassulacean acid metabolism in Australian vascular epiphytes and some related species. - Oecologia 57: 129-141, 1983. Go to original source...
    63. Wong, S. C., Hew, C. S.: Diffusive resistance, titratable acidity, and CO2 fixation in two tropical epiphytic ferns. - Am. Fern J. 66: 121-124, 1976. Go to original source...
    64. Zar, J. H. Biostatistical Analysis. Pp. 718. Prentice Hall, New Jersey 1996.
    65. Zotz, G. How prevalent is crassulacean acid metabolism among vascular epiphytes? - Oecologia 138: 184-192, 2004. Go to original source...
    66. Zotz, G., Ziegler, H. The occurrence of crassulacean acid metabolism among vascular epiphytes from Central Panama. - New Phytol. 137: 223-229, 1997. Go to original source...

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