Photosynthetica 2020, 58(5):1167-1177 | DOI: 10.32615/ps.2020.059

Differences in leaf anatomy, photosynthesis, and photoprotective strategies in the yellow-green leaf mutant and wild type of Rosa beggeriana Schrenk

S.H. YANG1, J.J. WEI1,2, F. YAN1, R.D. JIA1, X. ZHAO1, Y. GAN1, H. GE1
1 National Center of China for Flowers Improvement, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, 100081 Beijing, China
2 Beijing Botanic Garden, 100093 Beijing, China

Although the underlying mechanisms of chlorophyll biosynthesis have been intensively deciphered in model plants, leaf color mutants have not yet been fully studied in woody species. In this study, leaf anatomy, diurnal changes in photosynthesis and photoprotection were investigated in the yellow-green leaf (ygl) mutant of Rosa beggeriana. Chloroplast ultrastructure, pigment biosynthesis, and photosynthesis were impaired and led to photoinhibition at noon in the ygl mutant. The negative changes in leaf anatomy increased the risk of excess light absorption in the mutant. Moreover, the ygl mutant preferred to consume the excited energy through photorespiration rather than via heat dissipation. Finally, antioxidant defenses failed to scavenge reactive oxygen species and led to severe lipid peroxidation in the mutant. The results suggested that inhibition of photosynthesis in the ygl mutant of R. beggeriana was associated with the altered photoprotective strategies that involved in leaf anatomy, photorespiration, thermal dissipation, and antioxidant system.

Additional key words: chlorophyll fluorescence; gas exchange; heat dissipation; leaf color mutant; photodamage; rose.

Received: May 6, 2020; Revised: July 25, 2020; Accepted: August 11, 2020; Prepublished online: October 14, 2020; Published: December 8, 2020  Show citation

ACS AIP APA ASA Harvard Chicago Chicago Notes IEEE ISO690 MLA NLM Turabian Vancouver
YANG, S.H., WEI, J.J., YAN, F., JIA, R.D., ZHAO, X., GAN, Y., & GE, H. (2020). Differences in leaf anatomy, photosynthesis, and photoprotective strategies in the yellow-green leaf mutant and wild type of Rosa beggeriana Schrenk. Photosynthetica, 58(5), 1167-1177. doi: 10.32615/ps.2020.059
Share...
Download citation
  • BibTeX (.bib)
  • Bookends (.ris)
  • EasyBib (.ris)
  • EndNote (.enw)
  • EndNote 8 (.xml)
  • ISI WoS (.isi)
  • Medlars (.medlars)
  • Mendeley (.ris)
  • MODS (.xml)
  • Papers (.ris)
  • RefWorks (.txt)
  • RefManager (.ris)
  • RIS (.ris)
  • MS Word (.xml)
  • Zotero (.ris)
    • Open full article

    References

    1. Alexieva V., Sergiev I., Mapelli S., Karanov E.: The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. - Plant Cell Environ. 24: 1337-1344, 2001. Go to original source...
    2. Anderson J.V., Chevone B.I., Hess J.L.: Seasonal variation in the antioxidant system of eastern white pine needles: evidence for thermal dependence. - Plant Physiol. 98: 501-508,1992. Go to original source...
    3. Bendahmane M., Dubois A., Raymond O., Le Bris M.: Genetics and genomics of flower initiation and development in roses. - J. Exp. Bot. 64: 847-857, 2013. Go to original source...
    4. Bollivar D.W.: Recent advances in chlorophyll biosynthesis. - Photosynth. Res. 90: 173-194, 2006. Go to original source...
    5. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    6. Carriquí M., Douthe C., Molins A., Flexas J.: Leaf anatomy does not explain apparent short-term responses of mesophyll conductance to light and CO2 in tobacco. - Physiol. Plantarum 165: 604-618, 2019. Go to original source...
    7. Demmig-Adams B.: Linking the xanthophyll cycle with thermal energy dissipation. - Photosynth. Res. 76: 73, 2003. Go to original source...
    8. Deng X.J, Zhang H.Q., Wang Y. et al.: Mapped clone and functional analysis of leaf-color gene Ygl7 in a rice hybrid (Oryza sativa L. ssp. indica). - PLoS ONE 9: e99564, 2014. Go to original source...
    9. Elsheery N.I., Cao K.F.: Gas exchange, chlorophyll fluorescence, and osmotic adjustment in two mango cultivars under drought stress. - Acta Physiol. Plant. 30: 769-777, 2008. Go to original source...
    10. Elsheery N.I., Wilske B., Zhang J.L., Cao K.F.: Seasonal variations in gas exchange and chlorophyll fluorescence in the leaves of five mango cultivars in southern Yunnan, China. - J. Hortic. Sci. Biotech. 82: 855-862, 2007. Go to original source...
    11. Falbel T.G., Meehl J.B., Staehelin L.A.: Severity of mutant phenotype in a series of chlorophyll-deficient wheat mutants depends on light intensity and the severity of the block in chlorophyll synthesis. - Plant Physiol. 112: 821-832, 1996. Go to original source...
    12. Falbel T.G., Staehelin L.A., Adams III. W.W.: Analysis of xanthophyll cycle carotenoids and chlorophyll fluorescence in light intensity-dependent chlorophyll-deficient mutants of wheat and barley. - Photosynth. Res. 42: 191-202, 1994. Go to original source...
    13. Farquhar G.D, Sharkey T.D.: Stomatal conductance and photosynthesis. - Ann. Rev. Plant Physio. 33: 317-345, 1982. Go to original source...
    14. Filella I., Peñuelas J.: Altitudinal differences in UV absorbance, UV reflectance and related morphological traits of Quercus ilex and Rhododendron ferrugineum in the Mediterranean region. - Plant Ecol. 145: 157-165, 1999. Go to original source...
    15. Flexas J., Cano F.J., Carriquí M. et al.: CO2 diffusion inside photosynthetic organs. - In: Adams III W., Terashima I. (ed.): The Leaf: A Platform for Performing Photosynthesis. Pp. 163-208. Springer, Cham 2018. Go to original source...
    16. Foyer C.H., Noctor G.: Oxygen processing in photosynthesis: regulation and signaling. - New Phytol. 146: 359-388, 2000. Go to original source...
    17. Gang H., Li R., Zhao Y. et al.: Loss of GLK1 transcription factor function reveals new insights in chlorophyll biosynthesis and chloroplast development. - J. Exp. Bot. 70: 3125-3138, 2019. Go to original source...
    18. García-Plazaola J.I., Fernández-Marín B., Ferrio J.P. et al.: Endogenous circadian rhythms in pigment composition induce changes in photochemical efficiency in plant canopies. - Plant Cell Environ. 40: 1153-1162, 2017. Go to original source...
    19. Genty B., Briantais J.M., Baker N.R.: The relationship between quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - BBA-Gen. Subjects 990: 87-92, 1989. Go to original source...
    20. Gu C.Z., Robertson K.R.: Rosa (Rosaceae). - In: Wu Z.Y., Raven P.H. (ed.): Flora of China. Vol. 9. Pp. 339-381. Science Press, Beijing and Missouri Botanical Garden, St. Louis 2003.
    21. Heath R.L., Packer L.: Photoperoxidation in isolated chloro-plasts. I. Kinetics and stoichiometry of fatty acid peroxida-tion. - Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
    22. Huang S.W., Ge H.: Study on the use of Rosa beggeriana Schrenk as a source of cold resistance in rose breeding. - Acta Hortic. Sin. 16: 237-240, 1989. [In Chinese]
    23. Huang S.W., Ge H., Liang L.: A new cultivar of Beggeriana aurea rose by mutant breeding. - North. Hortic. 31: 128-130, 2007. [In Chinese]
    24. Jiang C.D., Gao H.Y., Zou Q. et al.: Leaf orientation, photorespiration and xanthophyll cycle protect young soybean leaves against high irradiance in field. - Environ. Exp. Bot. 55: 87-96, 2006. Go to original source...
    25. Li W., Tang S., Zhang S. et al.: Gene mapping and functional analysis of the novel leaf color gene SiYGL1 in foxtail millet [Setaria italica (L.) P. Beauv]. - Physiol. Plantarum 157: 24-37, 2016. Go to original source...
    26. Liu J., Wang J., Yao X. et al.: Fine mapping and photosynthetic characteristics of the lower chlorophyll b 1 mutant in rice (Oryza sativa L.). - Plant Breeding 134: 661-667, 2015. Go to original source...
    27. Liu X., Yu W., Wang G. et al.: Comparative proteomic and physiological analysis reveals the variation mechanisms of leaf coloration and carbon fixation in a xantha mutant of Ginkgo biloba L. - Int. J. Mol. Sci. 17: 1794, 2016. Go to original source...
    28. Osmond C.B.: What is photoinhibition? Some insights from comparisons of shade and sun plants. - In: Baker N.R., Bowyer J.R. (ed.): Photoinhibition of Photosynthesis: From Molecular Mechanisms to the Field. Pp. 1-24. Bios Scientific, Oxford 1994.
    29. Rehem B.C., Almeida A.A.F., Santos I.C. et al.: Photosynthesis, chloroplast ultrastructure, chemical composition and oxida-tive stress in Theobroma cacao hybrids with the lethal gene Luteus-Pa mutant. - Photosynthetica 49: 127-139, 2011. Go to original source...
    30. Reinbothe C., El Bakkouri M., Buhr F. et al.: Chlorophyll biosynthesis: spotlight on protochlorophyllide reduction. - Trends Plant Sci. 15: 614-624, 2010. Go to original source...
    31. Riederer M., Schreiber L.: Protecting against water loss: analysis of the barrier properties of plant cuticles. - J. Exp. Bot. 52: 2023-2032, 2001. Go to original source...
    32. ©troch M., Èajánek M., Kalina J., ©punda V.: Regulation of the excitation energy utilization in the photosynthetic apparatus of chlorina f2 barley mutant grown under different irradiances. - J. Photoch. Photobio. B 75: 41-50, 2004. Go to original source...
    33. Thayer S.S., Björkman O.: Leaf xanthopyll content and composition in sun and shade determined by HPLC. - Photosynth. Res. 23: 331-343, 1990. Go to original source...
    34. Wang L., Yue C., Cao H. et al.: Biochemical and transcriptome analyses of a novel chlorophyll-deficient chlorina tea plant cultivar. - BMC Plant Biol. 14: 352, 2014. Go to original source...
    35. Wu W., Elsheery N., Wei Q. et al.: Defective etioplasts observed in variegation mutants may reveal the light-independent regulation of white/yellow sectors of Arabidopsis leaves. - J. Integr. Plant Biol. 53: 846-857, 2011. Go to original source...
    36. Yang S.H., Li Q.X., Jia R.D. et al.: New rose cultivars Rosa 'Tianxiang', 'Tianshanbaixue', 'Tianshantaoyuan', 'Tianshanzhiguang' and 'Tianshanzhixing'. - Acta Hortic. Sin. 43: 2526-2534, 2016. [In Chinese] doi: 10.16420/j.issn.0513-353x.2015-0637. Go to original source...
    37. Yang S.H., Li S.C., Jia R.D. et al.: Primary construction of a genetic linkage map of Rosa beggeriana 'Aurea' × R. davu-rica. - Acta Hortic. Sin. 42: 2526-2534, 2015. [In Chinese] doi: 10.16420/j.issn.0513-353x.2015-0382. Go to original source...
    38. Yang S.H., Wang L.J., Li S.H.: Ultraviolet-B irradiation-induced freezing tolerance in relation to antioxidant system in winter wheat (Triticum aestivum L.) leaves. - Environ. Exp. Bot. 60: 300-307, 2007. Go to original source...
    39. Young M.A., Schorr P.: Preface. - In: Young M.A., Schorr P. (ed.): Modern Roses 12: The Comprehensive List of Roses in Cultivation or of Historical or Botanical Importance. Pp. 3. American Rose Society, Shreveport 2007.
    40. Zhong X.M., Sun S.F., Li F.H. et al.: Photosynthesis of a yellow-green mutant line in maize. - Photosynthetica 53: 499-505, 2015. Go to original source...

    Return to the content