Photosynthetica 2021, 59(1):49-60 | DOI: 10.32615/ps.2020.084

Stomatal, mesophyll and biochemical limitations to soil drought and rewatering in relation to intrinsic water-use efficiency in Manchurian ash and Mongolian oak

K. ZHU1, 2, F.H. YUAN1, A.Z. WANG1, J.B. WU1, D.X. GUAN1, C.J. JIN1, J. FLEXAS3, C.J. GONG1, 2, H.X. ZHANG1, 2, Y.S ZHANG4
1 Key Laboratory of Forest Ecology and Management, Institute of Applied Ecology, Chinese Academy of Sciences, 110016 Shenyang, China
2 University of Chinese Academy of Sciences, 100049 Beijing, China
3 Research Group on Plant Biology under Mediterranean Conditions, Department of Biology of the University of the Balearic Islands, Institute of Agroecology and Water Economy (INAGEA), Carretera de Valldemossa Km 7.5, 07122, Palma de Mallorca, Illes Balears, Spain
4 The Institute of Atmospheric Environment, China Meteorological Administration, 110166 Shenyang, China

The balance between stomatal and mesophyll conductance has been reported to directly influence intrinsic water-use efficiency (WUEi), but it is unclear whether variations in stomatal and mesophyll limitations (lsc and lm, respectively) affect WUEi during soil drought and rewatering. Limitations (lsc, lm and biochemical limitation, lb) and WUEi were measured in Manchurian ash (Fraxinus mandshurica Rupr.) and Mongolian oak (Quercus mongolica Fish. ex Ledeb) saplings exposed to two levels of water stress (moderate and severe) and rewatering in this study. The primary limiting factors for photosynthesis among lsc, lm, and lb and their correlations with WUEi were assessed. Both lsc and lm increased with water stress, while lb decreased, and during recovery, lsc and lm decreased, while lb increased. Photosynthesis limitations directly influenced WUEi, with WUEi being larger after rewatering than during water stress, increasing as biochemical capacity weakened at the expense of diffusional limitations (lsc and lm). Stomatal closure was more limiting to net photosynthesis in Mongolian oak than in Manchurian ash during the drought and recovery treatments; consistently higher WUEi was measured in the former species, possibly due to a more developed root system and larger leaf hydraulic conductance and stem vessel diameter in the former species.

Additional key words: biochemical capacity; diffusional limitations; intrinsic water-use efficiency; rewatering; soil drought.

Received: July 27, 2020; Revised: December 12, 2020; Accepted: December 21, 2020; Prepublished online: January 18, 2021; Published: March 18, 2021  Show citation

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ZHU, K., YUAN, F.H., WANG, A.Z., WU, J.B., GUAN, D.X., JIN, C.J., ... ZHANG, Y.S. (2021). Stomatal, mesophyll and biochemical limitations to soil drought and rewatering in relation to intrinsic water-use efficiency in Manchurian ash and Mongolian oak. Photosynthetica, 59(1), 49-60. doi: 10.32615/ps.2020.084
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