Photosynthetica 2021, 59(1):148-159 | DOI: 10.32615/ps.2021.006

Inhibition of photosynthesis in Melia azedarach and Ligustrum lucidum induced by manganese toxicity using OJIP chlorophyll a fluorescence transient

M.S. LIU2, X.H. HUANG2, R.J. WANG3, H.Y. XU2, F. ZHU1
1 College of Landscape Architecture and Art Design, Hunan Agricultural University, 410128 Changsha, China
2 College of Life Science and Technology, Central-South University of Forestry and Technology, 410004 Changsha, China
3 Guangxi Key Laboratory of Superior Trees Resource Cultivation, Guangxi Zhuang Autonomous 11 Region Forestry Research Institute, Nanning, China

Manganese (Mn) excess is a major abiotic stress for plant growth. In the present study, we investigated the effects of Mn on photosynthesis in Ligustrum lucidum and Melia azedarach leaves using chlorophyll fluorescence transients. Both plant species were exposed to two Mn concentrations (0.5 and 1 mmol) for 10 and 30 d. Results showed that excess Mn significantly inhibited photosynthesis. With the increase of Mn concentration and stress time, the inhibition was more serious. Mn stress impaired PSII at the donor and the acceptor side by damaging the oxygen-evolving complex and limiting electron transport downstream of QA in both trees. A significant decline in 820 nm reflection curve absorption was observed in M. azedarach, suggesting that the oxidization-reduction reactions in PSI were inhibited but this phenomenon was not observed in L. lucidum. Therefore, excess Mn impaired the whole electron transport chain associated with inactive PSII reaction centers in Ligustrum lucidum and inhibited oxidization-reduction reactions in PSI in M. azedarach.

Additional key words: energy conversion efficiency; heavy metal stress; JIP-test; OJIP curve.

Received: July 17, 2020; Revised: January 16, 2021; Accepted: January 18, 2021; Prepublished online: February 18, 2021; Published: March 18, 2021  Show citation

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LIU, M.S., HUANG, X.H., WANG, R.J., XU, H.Y., & ZHU, F. (2021). Inhibition of photosynthesis in Melia azedarach and Ligustrum lucidum induced by manganese toxicity using OJIP chlorophyll a fluorescence transient. Photosynthetica, 59(1), 148-159. doi: 10.32615/ps.2021.006
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