Photosynthetica 2021, 59(2):256-265 | DOI: 10.32615/ps.2021.019

Biochemical and photosystem characteristics of wild-type and Chl b-deficient mutant in tree peony (Paeonia suffruticosa)

L.X. ZHANG1, †, Q.S. CHANG2, †, X.G. HOU1, S.D. CHEN2, Q.M. ZHANG2, J.Z. WANG3, S.D. LIU4, S. LI1
1 College of Agriculture, Henan University of Science and Technology, 471000 Luoyang, China
2 College of Horticulture and Plant Protection, Henan University of Science and Technology, 471000 Luoyang, China
3 Luoyang Greening Management Center, 471000 Luoyang, China
4 Luoyang International Peony Garden, 471000 Luoyang, China

In order to explore the adaptive strategies of tree peony yl1 mutant in response to photooxidative stress, the changes of biochemical parameters, chlorophyll fluorescence, and the modulated 820-nm reflection of yl1 mutant and wild-type plants were compared. We found the activities of superoxide dismutase and catalase, the contents of soluble protein, proline, superoxide radicals, hydrogen peroxide, malondialdehyde, and relative electrical conductivity were significantly higher than those of the wild type. The photochemical efficiency of PSII significantly decreased, while the proportion of heat dissipation of yl1 mutant increased greatly. The donor and acceptor side and electron transfer of PSII were greatly inhibited. Meanwhile, the performance of PSI and coordination of two photosystems decreased markedly in yl1 mutant. The yl1 mutant could reduce the damage caused by photooxidative stress by increasing the activity of antioxidant enzymes, the content of some osmoregulatory substances, and the proportion of heat dissipation.

Additional key words: antioxidants; chlorophyll fluorescence; modulated 820-nm reflection; photosynthetic capacity; yl1 mutant.

Received: December 2, 2020; Revised: February 27, 2021; Accepted: March 15, 2021; Prepublished online: April 12, 2021; Published: June 29, 2021  Show citation

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ZHANG, L.X., CHANG, Q.S., HOU, X.G., CHEN, S.D., ZHANG, Q.M., WANG, J.Z., LIU, S.D., & LI, S. (2021). Biochemical and photosystem characteristics of wild-type and Chl b-deficient mutant in tree peony (Paeonia suffruticosa). Photosynthetica, 59(2), 256-265. doi: 10.32615/ps.2021.019
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    References

    1. Bates L.S., Waldren R.P., Teare I.D.: Rapid determination of free proline for water-stress studies. - Plant Soil 39: 205-207, 1973. Go to original source...
    2. Bradford M.M.: A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. - Anal. Biochem. 72: 248-254, 1976. Go to original source...
    3. Brestic M., Zivcak M., Kunderlikova K. et al.: Low PSI content limits the photoprotection of PSI and PSII in early growth stages of chlorophyll b-deficient wheat mutant lines. - Photosynth. Res. 125: 151-166, 2015. Go to original source...
    4. Cai H., Xie P., Zeng W. et al.: Root-specific expression of rice OsHMA3 reduces shoot cadmium accumulation in transgenic tobacco. - Mol. Breeding 39: 49, 2019. Go to original source...
    5. Chai C., Fang J., Liu Y. et al.: ZEBRA2, encoding a carotenoid isomerase, is involved in photoprotection in rice. - Plant Mol. Biol. 75: 211-221, 2011. Go to original source...
    6. Chang Q.S., Zhang L.X., Hou X.G. et al.: The anatomical, physiological and molecular analysis of a chlorophyll-deficient mutant in tree peony (Paeonia suffruticosa). - Photosynthetica 57: 724-730, 2019. Go to original source...
    7. Chen K.Y., Li C.N., Cheng M.M. et al.: [Chloroplast ultrastructure and chlorophyll fluorescence characteristics of three cultivars of Pseudosasa japonica.] - Chin. Bull. Bot. 53: 509-518, 2018. [In Chinese] Go to original source...
    8. Chen L., Yuan Q.Z.: [Study on Luoyang tourism climate comfort based on entropy method.] - J. Luoyang Normal Univ. 37: 20-25, 2018. [In Chinese]
    9. Chen X.X.: [Formative mechanism and maintenance charac-teristics of leaf color chimera and mutants of Anthurium andreaeanum 'Sonate'.] Master Thesis. Nanjing Agricultural University, Nanjing 2009. [In Chinese]
    10. Chu J.T.: [Study on leaf color variation mechanism of a new cultivar 'Golden qilu' in Acer buergerianum Miq.] Master Thesis. Shandong Agricultural University, Taian 2020. [In Chinese]
    11. D±browski P., Kalaji M.H., Baczewska A.H. et al.: Delayed chlorophyll a fluorescence, MR 820, and gas exchange changes in perennial ryegrass under salt stress. - J. Lumin. 183: 322-333, 2017. Go to original source...
    12. D±browski P., Pawlu¶kiewicz B., Baczewska A.H. et al.: Chlorophyll a fluorescence of perennial ryegrass (Lolium perenne L.) varieties under long term exposure to shade. - Zemdirbyste 102: 305-312, 2015. Go to original source...
    13. Demmig-Adams B., Adams III W.W., Barker D.H. et al.: Using chlorophyll fluorescence to assess the fraction of absorbed light allocated to thermal dissipation of excess excitation. - Physiol. Plantarum 98: 253-264, 1996. Go to original source...
    14. Deng B.L.: [Albino plant and higher endogenous reactive oxygen species toxicity.] Master Thesis. Lanzhou University, Lanzhou 2009. [In Chinese]
    15. Dong F., Wang C., Sun X. et al.: Sugar metabolic changes in protein expression associated with different light quality combinations in tomato fruit. - Plant Growth Regul. 88: 267-282, 2019. Go to original source...
    16. Du Q., Wang N., Zhao X.H. et al.: [Effects of potassium deficiency on photosynthesis and performance of photosystem II in maize seedling stage.] - J. Nucl. Agric. Sci. 33: 592-599, 2019. [In Chinese]
    17. Genty B., Briantais J.M., Baker N.R.: The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence. - BBA-Gen. Subjects 990: 87-92, 1989. Go to original source...
    18. Guo N.H.: [Gene mapping and physiological characteristics analysis of a zebra leaf mutant l139 in rice.] Master Thesis. Shenyang Agricultural University, Shenyang 2020. [In Chinese]
    19. Guo Y.Y., Tian S.S., Liu S.S. et al.: Energy dissipation and antioxidant enzyme system protect photosystem II of sweet sorghum under drought stress. - Photosynthetica 56: 861-872, 2018. Go to original source...
    20. Hu W.H., Xiao Y.A., Yu J.Q. et al.: [Effects of different light intensity after low night temperature stress on PSII functions and absorbed light allocation in leaves of Ficus macrocarpa.] -Bull. Bot. Res. 25: 159-162, 2005. [In Chinese]
    21. Hu Z.: [Gene cloning and functional analysis of yll conferring the leaf color through characterizing the rice yl1 mutant.] Master Thesis. Yangzhou university, Yangzhou 2017. [In Chinese]
    22. Johal N., Kaur J., Grewal S.K. et al.: Physiological and bio-chemical responses of chickpea accessions at reproductive stage under receding moisture conditions. - Agr. Res. 9: 554-567, 2020. Go to original source...
    23. Kalaji H.M., Schansker G., Brestic M. et al.: Frequently asked questions about chlorophyll fluorescence, the sequel. - Photosynth. Res. 132: 13-66, 2017. Go to original source...
    24. Kalaji H.M., Schansker G., Ladle R.J. et al.: Frequently asked questions about in vivo chlorophyll fluorescence: Practical issues. - Photosynth. Res. 122: 121-158, 2014. Go to original source...
    25. Krieger-Liszkay A., Kós P.B., Hideg E.: Superoxide anion radicals generated by methylviologen in photosystem I damage photosystem II. - Physiol. Plantarum 142: 17-25, 2011. Go to original source...
    26. Labudda M., Tokarz K., Tokarz B. et al.: Reactive oxygen species metabolism and photosynthetic performance in leaves of Hordeum vulgare plants co-infested with Heterodera filipjevi and Aceria tosichella. - Plant Cell Rep. 39: 1719-1741, 2020. Go to original source...
    27. Lai Y., Fu Q.S., Lv J.C. et al.: [Analysis of physiological characteristics and chloroplast ultrastructure of a new leaf color mutant in melon.] - J. Sichuan Agric. Univ. 36: 372-379, 2018. [In Chinese]
    28. Li G., Gao H.Y., Liu P. et al.: [Effects of nitrogen fertilization on photosynthetic performance in maize leaf at grain filling stage.] - Plant Nutr. Fert. Sci. 16: 536-542, 2010b. [In Chinese]
    29. Li G., Gao H.Y., Zhao B. et al.: [Effects of drought stress on activity of photosystems in leaves of maize at grain filling stage.] - Acta Agron. Sin. 35: 1916-1922, 2009. [In Chinese] Go to original source...
    30. Li P.M., Gao H.Y., Strasser R.J.: [Application of the fast chlorophyll fluorescence induction dynamics analysis in photosynthesis study.] - J. Plant Physiol. Mol. Biol. 31: 559-566, 2005. [In Chinese]
    31. Li Q., Chen L.S., Jiang H.X. et al.: Effects of manganese-excess on CO2 assimilation, ribulose-1,5-bisphosphate carboxylase/oxygenase, carbohydrates and photosynthetic electron transport of leaves, and antioxidant systems of leaves and roots in Citrus grandis seedlings. - BMC Plant Biol. 10: 42, 2010a. Go to original source...
    32. Li W.Q., Gao B., Yang J. et al.: [Physiological characteristic analysis of a new leaf color yellow mutant in cucumber.] - Acta Agric. Bor.-Occident. Sin. 24: 98-103, 2015. [In Chinese]
    33. Li X., Brestic M., Tan D.X. et al.: Melatonin alleviates low PS I-limited carbon assimilation under elevated CO2 and enhances the cold tolerance of offspring in chlorophyll b-deficient mutant wheat. - J. Pineal Res. 64: e12453, 2018. Go to original source...
    34. Li Y.: [Physiological characteristics and genetic analysis of leaf color mutant in cucumber.] Master Thesis. Sichuan Agricultural University, Chendu 2016. [In Chinese]
    35. Liu A.R., Wang M.M., Liu D.L. et al.: Arbuscular mycorrhizal fungus alleviates chilling stress by boosting redox poise and antioxidant potential of tomato seedlings. - J. Plant Growth Regul. 35: 109-120, 2016b. Go to original source...
    36. Liu C., Duan Y., An X. et al.: [Comparison of main physiological characteristics, chloroplast morphology and ultra-structure of the yellow-green wheat (Triticum aestivum) near-isogenic lines.] - J. Agric. Biotech. 24: 806-814, 2016a. [In Chinese]
    37. Liu J., Yang Y.H., Hao Y.C. et al.: [Effects of photosynthetic electron transport chain of maize leaves under drought stress and re-watering.] - Plant Physiol. J. 53: 1877-1884, 2017. [In Chinese]
    38. Liu M.J., Sui X.Q., An S.Z.: [The effect of dehydration on Cynodon dactylon L. leaf photosynthetic apparatus.] - Acta Agr. Sin. 26: 441-446, 2018. [In Chinese]
    39. Liu M.Y., Lu Y., Zhao J.J. et al.: [HRM identification and chlorophyll fluorescence characteristics on leaf color mutants in chinese cabbage.] - Acta Hortic. Sin. 41: 2215-2224, 2014. [In Chinese]
    40. Lv D.H.: [Study on photosynthetic, physiological and biochemical characteristics in leaf color mutants of rice (Oryza sativa L.).] Master Thesis. Southwest University, Chongqing 2010. [In Chinese]
    41. Parmoon G., Ebadi A., Jahanbakhsh S. et al.: Assessing photo-synthetic performance of fennel (Foeniculum vulgare Mill) influenced by plant growth regulators and drought stress imposed at vegetative and reproductive stages. - Ital. J. Agron. 14: 93-100, 2019. Go to original source...
    42. Pfündel E.E., Klughammer C., Meister A., Cerovic Z.G.: Deriving fluorometer-specific values of relative PSI fluorescence intensity from quenching of FO fluorescence in leaves of Arabidopsis thaliana and Zea mays. - Photosynth. Res. 114: 189-206, 2013. Go to original source...
    43. Qiu N.W., Zhou F., Wang Y. et al.: [Comparison on characteristics of the fast chlorophyll fluorescence induction kinetics between Pinus species and Populus species.] - Sci. Silva. Sin. 49: 136-143, 2013. [In Chinese]
    44. Rastogi A., Kovar M., He X. et al.: JIP-test as a tool to identify salinity tolerance in sweet sorghum genotypes. - Photosynthetica 58: 518-528, 2020. Go to original source...
    45. Ru G.X., Liu X.N., Zhu X.H. et al.: [Physiological characteristic analysis of etiolation mutant in Paulownia fortnnei.] - J. Nanjing Forest. Univ. 60: 181-185, 2017. [In Chinese]
    46. Souza A.F.C, Martins J.P.R., Gontijo A.B.P.L., Falqueto A.R.: Selenium improves the transport dynamics and energy conservation of the photosynthetic apparatus of in vitro grown Billbergia zebrina (Bromeliaceae). - Photosynthetica 57: 931-941, 2019. Go to original source...
    47. Strasser R.J., Tsimilli-Michael M., Qiang S., Goltsev V.: Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. - BBA-Bioenergetics 1797: 1313-1326, 2010. Go to original source...
    48. Strasser R.J., Tsimilli-Michael M., Srivastava A.: Analysis of the chlorophyll a fluorescence transient. - In: Papageorgiou G.C., Govindjee (ed.): Chlorophyll a Fluorescence: A Signature of Photosynthesis. Advances in Photosynthesis and Respiration. Pp. 321-362. Springer, Dordrecht 2004. Go to original source...
    49. Sun Y.P., Liu J., Cao R.X. et al.: Effects of 5-aminolevulinic acid treatment on photosynthesis of strawberry. - Photosynthetica 55: 276-284, 2017. Go to original source...
    50. Takahashi S., Murata N.: How do environmental stresses accelerate photoinhibition? - Trends Plant Sci. 13: 178-182, 2008. Go to original source...
    51. Velikova V., Yordanov I., Edreva A.: Oxidative stress and some antioxidant systems in acid rain-treated bean plants: protective role of exogenous polyamines. - Plant Sci. 151: 59-66, 2000. Go to original source...
    52. Wang N., Tian Y.W., Chen H.: Seed dormancy and germination response of Aegilops tauschii to exogenous application of GA3 and warm water. - Int. J. Agric. Biol. 24: 885-890, 2020.
    53. Wang Y., Zheng W., Zheng W.J. et al.: Physiological and transcriptomic analyses of a yellow-green mutant with high photosynthetic efficiency in wheat (Triticum aestivum L.). - Funct. Integr. Genomic. 8: 175-194, 2018. Go to original source...
    54. Wu M.J., Yan Y.R., Wang Y.Q. et al.: Arbuscular mycorrhizal fungi for vegetable (VT) enhance resistance to Rhizoctonia solani in watermelon by alleviating oxidative stress. - Biol. Control 152: 104433, 2021. Go to original source...
    55. Xiao H.G., Yang H.W., Rao Y. et al.: [Analysis of chloroplast ultrastructure, stomatal characteristic parameters and photo-synthetic characteristics of chlorophyll-reduced mutant in Brassica napus L.] - Sci. Agr. Sin. 46: 715-727, 2013. [In Chinese]
    56. Yang C., Zhang Y.Y., Fang Z.Y. et al.: [Photosynthetic physiological characteristics and chloroplast ultrastructure of yellow leaf mutant YL-1 in cabbage.] - Acta Hortic. Sin. 41: 1133-1144, 2014. [In Chinese]
    57. Yang J., Kong Q., Xiang C.: Effects of low night temperature on pigments, Chl a fluorescence and energy allocation in two bitter gourd (Momordica charantia L.) genotypes. - Acta Physiol. Plant. 31: 285-293, 2009. Go to original source...
    58. Zhang D., Zhang Q.S., Yang X.Q.: Adaptive strategies of Zostera japonica photosynthetic electron transport in response to thermal stress. - Mar. Biol. 164: 35, 2017. Go to original source...
    59. Zhang H., Chen Y., Niu Y. et al.: Characterization and fine mapping of a leaf yellowing mutant in common wheat. - Plant Growth Regul. 92: 233-247, 2020. Go to original source...
    60. Zhang L.M., Lv W.Y., Zhang L.X.: [Agronomic traits and physiological-biochemical characteristics of light-green leaf mutant sll1 of Sorghum bicolor.] - Plant Physiol. J. 50: 1401-1405, 2014. [In Chinese]
    61. Zhang Z.S., Jia Y.J., Gao H.Y. et al.: Characterization of PSI recovery after chilling-induced photoinhibition in cucumber (Cucumis sativus L.). - Planta 234: 883-889, 2011. Go to original source...

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