Photosynthetica 2021, 59(2):294-302 | DOI: 10.32615/ps.2021.027

The changes of PSII supercomplex stoichiometry in egy1 mutants are related to chlorophyll b deficiency

M. ADAMIEC, L. MISZTAL, M. CIESIELSKA, R. LUCIŃSKI
Department of Plant Physiology, Institute of Experimental Biology, Faculty of Biology, Adam Mickiewicz University, Uniwersytetu Poznańskiego 6, 61-616 Poznań, Poland

EGY1 is a chloroplast metalloprotease, the physiological role of which remains elusive. The changes observed in physiology and gene expression in egy1 mutants indicate that lack of the protease leads to yellow-green phenotype, changes in stoichiometry in PSII complexes, and early senescence. However, the knowledge concerning the role of the EGY1 to maintain the PSII function remains elusive. The aim of our study was to gain a deeper insight into the role of EGY1 protease in maintaining proper stoichiometry of PSII complexes. We applied the blue native electrophoresis technique as well as the immunoblotting method to investigate the abundance of PSII supercomplexes and selected individual PSII apoproteins in two Arabidopsis thaliana egy1 mutant lines. We also performed analyses of photosynthetic pigment content using DMSO assay. All analyses were performed in three biological replicates. Our results revealed reductions in contents of LHCII trimers and monomers in both egy1 mutant lines, as well as lower accumulation levels of Lhcb1 and Lhcb2 (but not Lhcb3) apoproteins. These changes were accompanied by an increased chlorophyll a/b ratio. We conclude that the observed pattern of changes in PSII stoichiometry is related to chlorophyll b deficiency. This reduction of chlorophyll b content is not, however, related to chlorophyllide a oxygenase abundance.

Additional key words: Arabidopsis thaliana; chloroplasts; EGY1; intramembrane proteases; photosystem II.

Received: February 8, 2021; Revised: March 30, 2021; Accepted: April 19, 2021; Prepublished online: May 13, 2021; Published: June 29, 2021  Show citation

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ADAMIEC, M., MISZTAL, L., CIESIELSKA, M., & LUCIŃSKI, R. (2021). The changes of PSII supercomplex stoichiometry in egy1 mutants are related to chlorophyll b deficiency. Photosynthetica, 59(2), 294-302. doi: 10.32615/ps.2021.027
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