Photosynthetica 2021, 59(SI):399-408 | DOI: 10.32615/ps.2021.026

A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers

B. PADHI1, G. CHAUHAN1, D. KANDOI1, A. STIRBET2, B.C. TRIPATHY1, 3, G. GOVINDJEE4
1 School of Life Sciences, Jawaharlal Nehru University, New Mehrauli Road, 110067 New Delhi, India
2 Anne Burras Lane, Newport News, VA 23606, USA
3 Department of Biotechnology, Sharada University, 201306 Greater Noida, UP, India
4 Department of Plant Biology, Department of Biochemistry, and Center of Biophysics & Quantitative Biology, University of Illinois at Urbana-Champaign, Urbana, IL 61801, USA

We provide here a general introduction on chlorophyll (Chl) a fluorescence, then we present our measurements on fast (< 1 s) induction curves (the so-called OJIP transients) on dark-adapted intact leaves of Arabidopsis thaliana, under five different light intensities [in the range of ~ 500 to ~ 3,000 µmol(photons) m‒2 s‒1] using two different instruments: Handy PEA (Hansatech Instruments, UK; excitation light, 650 nm) and FluorPen (model FP-110; Photon Systems Instruments, The Czech Republic; excitation light, 470 nm). We then discuss the observed differences in the OJIP curves, as well as in Fo (F20μs, F50μs, or the extrapolated Ft→0), FP (the peak), and the ratios FP/Fo, and Fv (= FP ‒ Fo)/FP in terms of differences in excitation light intensity and absorptance (or absorbance) of the excitation light by the leaves, and other factors, as well as the data available in the literature. We suggest that such measurements be accompanied, in the future, by parallel measurements on Chl a fluorescence imaging, an area pioneered by Hartmut K. Lichtenthaler.

Additional key words: Arabidopsis thaliana; blue and red excitation light; fluorescence imaging; OJIP transient.

Received: November 22, 2020; Revised: April 8, 2021; Accepted: April 15, 2021; Prepublished online: May 10, 2021; Published: July 23, 2021  Show citation

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PADHI, B., CHAUHAN, G., KANDOI, D., STIRBET, A., TRIPATHY, B.C., & GOVINDJEE, G. (2021). A comparison of chlorophyll fluorescence transient measurements, using Handy PEA and FluorPen fluorometers. Photosynthetica, 59(SPECIAL ISSUE), 399-408. doi: 10.32615/ps.2021.026
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