Photosynthetica 2021, 59(4):600-605 | DOI: 10.32615/ps.2021.051

Photoprotective roles of ascorbate and PSII cyclic electron flow in the response of the seagrass Zostera marina to oxygen-evolving complex photoinactivation

W. ZHAO, Q.S. ZHANG, Y. TAN, Z. LIU, M.Y. MA, M.X. WANG, C.Y. LUO
Ocean School, Yantai University, 264005 Yantai, China

The oxygen-evolving complex (OEC) of Zostera marina is prone to deactivation under visible light, which results in a formation of the long-lived radical P680+. The mechanism to prevent damage caused by P680+ remains unclear. In this study, following light exposure, the upregulation in ascorbate (AsA) content and the presence of PSII cyclic electron flow (PSII-CEF) provide evidence that AsA and PSII-CEF donate electrons to PSII. Furthermore, a factorial design experiment with different combinations of inhibition of AsA and PSII-CEF demonstrates that both inhibition treatments lead to decreases in maximal photochemical yield of PSII, increases in relative variable fluorescence at the K-step, as well as the net loss of PSII reaction center proteins and further degradation of OEC peripheral proteins. These results suggest that AsA and PSII-CEF play photoprotective roles by providing electrons to efficiently prevent damage to PSII from the highly oxidizing radical P680+ in Z. marina.

Additional key words: alternative electron donor; ascorbate; cstylePSII cyclic electron flow; cstyle

Received: July 4, 2021; Revised: October 4, 2021; Accepted: October 27, 2021; Prepublished online: November 18, 2021; Published: December 17, 2021  Show citation

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ZHAO, W., ZHANG, Q.S., TAN, Y., LIU, Z., MA, M.Y., WANG, M.X., & LUO, C.Y. (2021). Photoprotective roles of ascorbate and PSII cyclic electron flow in the response of the seagrass Zostera marina to oxygen-evolving complex photoinactivation. Photosynthetica, 59(4), 600-605. doi: 10.32615/ps.2021.051
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    References

    1. Ananyev G., Gates C., Dismukes G.C.: The oxygen quantum yield in diverse algae and cyanobacteria is controlled by partitioning of flux between linear and cyclic electron flow within photosystem II. - BBA-Bioenergetics 1857: 1380-1391, 2016. Go to original source...
    2. Ananyev G., Gates C., Kaplan A., Dismukes G.C.: Photosystem II-cyclic electron flow powers exceptional photoprotection and record growth in the microalga Chlorella ohadii. - BBA-Bioenergetics 1858: 873-883, 2017. Go to original source...
    3. Barber J., De Las Rivas J.: A functional model for the role of cytochrome b559 in the protection against donor and acceptor side photoinhibition. - P. Natl. Acad. Sci. USA 90: 10942-10946, 1993. Go to original source...
    4. Beer S., Björk M., Beardall J.: Photosynthesis in the Marine Environment. Pp. 224. Wiley-Blackwell, Oxford 2014.
    5. Brestic M., Zivcak M., Kalaji H.M. et al.: Photosystem II thermostability in situ: Environmentally induced acclimation and genotype-specific reactions in Triticum aestivum L. - Plant Physiol. Bioch. 57: 93-105, 2012. Go to original source...
    6. Fristedt R., Willig A., Granath P. et al.: Phosphorylation of photosystem II controls functional macroscopic folding of photosynthetic membranes in Arabidopsis. - Plant Cell 21: 3950-3964, 2009. Go to original source...
    7. Garmier M., Carroll A.J., Delannoy E. et al.: Complex I dysfunction redirects cellular and mitochondrial metabolism in Arabidopsis. - Plant Physiol. 148: 1324-1341, 2008. Go to original source...
    8. Gururani M.A., Venkatesh J., Tran L.S.P.: Regulation of photosynthesis during abiotic stress-induced photoinhibition. - Mol. Plant 8: 1304-1320, 2015. Go to original source...
    9. Jiang M., Ren L., Lian H. et al.: Novel insight into the mechanism underlying light-controlled anthocyanin accumulation in eggplant (Solanum melongena L.). - Plant Sci. 249: 46-58, 2016. Go to original source...
    10. Jorrin-Novo J.V.: Plant proteomics methods and protocols. - In: Jorrin-Novo J., Komatsu S., Weckwerth W., Wienkoop S. (ed.): Plant Proteomics. Methods in Molecular Biology. Vol. 1072. Humana Press, Totowa 2014. Go to original source...
    11. Lavaud J.: Fast Regulation of Photosynthesis in Diatoms: Mechanisms, Evolution and Ecophysiology. - Funct. Plant Sci. Biotechonol. 1: 267-287, 2007.
    12. Li Y.Y., Mao K., Zhao C. et al.: Molecular cloning and functional analysis of a blue light receptor gene MdCRY2 from apple (Malus domestica). - Plant Cell Rep. 32: 555-566, 2013. Go to original source...
    13. Magnuson A., Rova M., Mamedov F. et al.: The role of cytochrome b559 and tyrosineD in protection against photoinhibition during in vivo photoactivation of Photosystem II. - BBA-Bioenergetics 1411: 180-191, 1999. Go to original source...
    14. Mano J.I., Eva H., Asada K.: Ascorbate in thylakoid lumen functions as an alternative electron donor to photosystem II and photosystem I. - Arch. Biochem. Biophys. 429: 71-80, 2004. Go to original source...
    15. Millar A.H., Mittova V., Kiddle G. et al.: Control of ascorbate synthesis by respiration and its implications for stress responses. - Plant Physiol. 133: 443-447, 2003. Go to original source...
    16. Olsen J.L., Rouzé P., Verhelst B. et al.: The genome of the seagrass Zostera marina reveals angiosperm adaptation to the sea. - Nature 530: 331-335, 2016. Go to original source...
    17. Porra R.J., Thompson W.A., Kriedemann P.E.: Determination of accurate extinction coefficients and simultaneous equations for assaying chlorophylls a and b extracted with four different solvents: verification of the concentration of chlorophyll standards by atomic absorption spectroscopy. - BBA-Bioenergetics 975: 384-394, 1989. Go to original source...
    18. Shinopoulos K.E., Brudvig G.W.: Cytochrome b559 and cyclic electron transfer within photosystem II. - BBA-Bioenergetics 1817: 66-75, 2012. Go to original source...
    19. Strasser B.J.: Donor side capacity of Photosystem II probed by chlorophyll a fluorescence transients. - Photosynth. Res. 52: 147-155, 1997. Go to original source...
    20. Strasser R.J., Tsimilli-Michael M., Qiang S., Goltsev V.: Simultaneous in vivo recording of prompt and delayed fluorescence and 820-nm reflection changes during drying and after rehydration of the resurrection plant Haberlea rhodopensis. - BBA-Bioenergetics 1797: 1313-1326, 2010. Go to original source...
    21. Tan Y., Zhang Q.S., Zhao W. et al.: The highly efficient NDH-dependent photosystem I cyclic electron flow pathway in the marine angiosperm Zostera marina. - Photosynth. Res. 144: 49-62, 2020a. Go to original source...
    22. Tan Y., Zhang Q.S., Zhao W. et al.: Chlororespiration serves as photoprotection for the photo-inactivated oxygen-evolving complex in Zostera marina, a marine angiosperm. - Plant Cell Physiol. 61: 1517-1529, 2020b. Go to original source...
    23. Tóth S.Z., Nagy V., Puthur J.T. et al.: The physiological role of ascorbate as photosystem II electron donor: protection against photoinactivation in heat-stressed leaves. - Plant Physiol. 156: 382-392, 2011. Go to original source...
    24. Tóth S.Z., Puthur J.T., Nagy V., Garab G.: Experimental evidence for ascorbate-dependent electron transport in leaves with inactive oxygen-evolving complexes. - Plant Physiol. 149: 1568-1578, 2009. Go to original source...
    25. Tyystjärvi E.: Photoinhibition of Photosystem II and photodamage of the oxygen evolving manganese cluster. - Coordin. Chem. Rev. 252: 361-376, 2008. Go to original source...
    26. Wagner H., Jakob T., Lavaud J., Wilhelm C.: Photosystem II cycle activity and alternative electron transport in the diatom Phaeodactylum tricornutum under dynamic light conditions and nitrogen limitation. - Photosynth. Res. 128: 151-161, 2016. Go to original source...
    27. Walker D.A., Cerovic Z.G., Robinson S.P.: Isolation of intact chloroplasts: General principles and criteria of integrity. - Method. Enzymol. 148: 145-157, 1987. Go to original source...
    28. Wissler L., Codoñer F.M., Gu J. et al.: Back to the sea twice: identifying candidate plant genes for molecular evolution to marine life. - BMC Evol. Biol. 11: 8, 2011. Go to original source...
    29. Yang X.Q., Zhang Q.S., Zhang D., Sheng Z.T.: Light intensity dependent photosynthetic electron transport in eelgrass (Zostera marina L.). - Plant Physiol. Bioch. 113: 168-176, 2017. Go to original source...
    30. Zhang Z., Jia Y., Gao H. et al.: Characterization of PSI recovery after chilling-induced photoinhibition in cucumber (Cucumis sativus L.) leaves. - Planta 234: 883-889, 2011. Go to original source...
    31. Zhao W., Yang X.-Q., Zhang Q.-S. et al.: Photoinactivation of the oxygen-evolving complex regulates the photosynthetic strategy of the seagrass Zostera marina. - J. Photoch. Photobio. B 222: 112259, 2021a. Go to original source...
    32. Zhao W., Zhang Q.S., Tan Y. et al.: An underlying mechanism of qE deficiency in marine angiosperm Zostera marina. - Photosynth. Res. 148: 87-99, 2021b. Go to original source...

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